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The map label for this gene is plcB

Identifier: 148662178

GI number: 148662178

Start: 2638685

End: 2640223

Strand: Reverse

Name: plcB

Synonym: MRA_2370

Alternate gene names: 148662178

Gene position: 2640223-2638685 (Counterclockwise)

Preceding gene: 148662179

Following gene: 148662177

Centisome position: 59.73

GC content: 62.7

Gene sequence:

>1539_bases
ATGACCCGCCGACAATTTTTTGCCAAAGCCGCCGCCGCTACCACCGCCGGGGCCTTCATGTCCTTGGCTGGTCCGATTAT
CGAAAAAGCCTACGGAGCGGGGCCTTGCCCCGGACATTTGACCGACATCGAGCACATCGTGTTGTTGATGCAGGAGAATC
GGTCATTCGATCACTACTTCGGCACTCTTTCTGACACCCGCGGGTTCGATGACACCACCCCGCCGGTGGTATTCGCGCAG
TCGGGCTGGAACCCGATGACACAGGCGGTTGACCCCGCCGGCGTCACCCTTCCATATCGCTTCGACACCACCCGGGGCCC
GCTGGTCGCCGGCGAATGCGTCAACGACCCGGACCACAGCTGGATCGGCATGCACAACTCGTGGAACGGCGGCGCCAACG
ACAACTGGCTGCCGGCGCAGGTCCCGTTCAGTCCGTTGCAGGGCAACGTGCCGGTCACGATGGGTTTCTACACGCGTCGT
GACCTGCCCATTCACTACCTGCTAGCCGACACGTTCACGGTCTGCGACGGCTATTTTTGCTCGCTGCTGGGCGGGACCAC
GCCCAACCGGCTCTACTGGATGAGCGCCTGGATCGACCCCGACGGCACTGACGGCGGGCCGGTGCTGATCGAGCCCAATA
TCCAACCTCTGCAGCACTACAGCTGGCGCATCATGCCGGAGAACCTCGAAGATGCCGGGGTCAGCTGGAAGGTGTACCAA
AACAAATTGCTGGGGGCTCTCAACAACACCGTCGTCGGCTACAACGGGCTGGTCAATGACTTCAAGCAGGCCGCGGATCC
GAGGTCGAATCTGGCCCGTTTCGGCATCTCACCGACCTACCCGCTGGACTTCGCCGCCGACGTCAGAAACAACCGATTAC
CCAAGGTCTCCTGGGTGCTGCCCGGGTTTCTGCTGTCCGAACATCCCGCATTCCCGGTGAACGTCGGCGCCGTTGCAATT
GTCGATGCACTGCGGATCTTGCTGTCCAACCCTGCGGTGTGGGAAAAGACCGCGCTGATCGTCAACTACGACGAGAACGG
CGGGTTCTTCGACCACGTCGTGCCGCCGACACCGCCGCCGGGGACGCCCGGCGAATTCGTCACGGTCCCCGACATCGACT
CGGTGCCCGGCTCGGGTGGCATTCGTGGGCCGATCGGTCTGGGGTTTCGCGTCCCTTGCCTCGTCATCTCTCCCTATAGC
CGCGGCCCACTGATGGTCCACGACACCTTCGATCACACGTCAACGCTGAAGCTCATCCGCGCGCGGTTCGGCGTCCCGGT
TCCCAATCTCACCGCTTGGCGGGACGCGACGGTCGGCGATATGACTTCGACGTTCAACTTCGCCGCTCCGCCGAACCCGT
CGAAACCCAATCTGGACCACCCGCGGCTCAATGCGCTTCCGAAGTTGCCGCAGTGCGTGCCCAACGCAGTACTGGGAACG
GTGACCAAGACGGCAATTCCCTATCGGGTGCCATTCCCACAGTCGATGCCCACCCAAGAAACCGCACCCACTCGGGGGAT
TCCCAGCGGTCTCTGTTGA

Upstream 100 bases:

>100_bases
GCAAGGCATAGCCGATCGCGGCCGCAACTATACCGTGATCAGCAATGGCCGTGTGGGCCGTCAAGGAGGGCGCGTGGGGA
GCGAACACCCCGTCGACGGA

Downstream 100 bases:

>100_bases
CCCACAGTCGGCGATGGCCGCGACGGTAAGCCGAAATACACGAGGGAGAGCCGATGTGGTGAGCCAAGGTGCATTCGCCG
GAATGTCACGCCGAGCATTC

Product: membrane-associated phospholipase C 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSDTRGFDDTTPPVVFAQ
SGWNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHSWIGMHNSWNGGANDNWLPAQVPFSPLQGNVPVTMGFYTRR
DLPIHYLLADTFTVCDGYFCSLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLEDAGVSWKVYQ
NKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNNRLPKVSWVLPGFLLSEHPAFPVNVGAVAI
VDALRILLSNPAVWEKTALIVNYDENGGFFDHVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIGLGFRVPCLVISPYS
RGPLMVHDTFDHTSTLKLIRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQCVPNAVLGT
VTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC

Sequences:

>Translated_512_residues
MTRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSDTRGFDDTTPPVVFAQ
SGWNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHSWIGMHNSWNGGANDNWLPAQVPFSPLQGNVPVTMGFYTRR
DLPIHYLLADTFTVCDGYFCSLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLEDAGVSWKVYQ
NKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNNRLPKVSWVLPGFLLSEHPAFPVNVGAVAI
VDALRILLSNPAVWEKTALIVNYDENGGFFDHVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIGLGFRVPCLVISPYS
RGPLMVHDTFDHTSTLKLIRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQCVPNAVLGT
VTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC
>Mature_511_residues
TRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSDTRGFDDTTPPVVFAQS
GWNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHSWIGMHNSWNGGANDNWLPAQVPFSPLQGNVPVTMGFYTRRD
LPIHYLLADTFTVCDGYFCSLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLEDAGVSWKVYQN
KLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNNRLPKVSWVLPGFLLSEHPAFPVNVGAVAIV
DALRILLSNPAVWEKTALIVNYDENGGFFDHVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIGLGFRVPCLVISPYSR
GPLMVHDTFDHTSTLKLIRARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQCVPNAVLGTV
TKTAIPYRVPFPQSMPTQETAPTRGIPSGLC

Specific function: Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHLB_MYCTU (P95246)

Other databases:

- EMBL:   U49511
- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   G70662
- RefSeq:   NP_216866.1
- RefSeq:   NP_336894.1
- ProteinModelPortal:   P95246
- EnsemblBacteria:   EBMYCT00000000881
- EnsemblBacteria:   EBMYCT00000069287
- GeneID:   885999
- GeneID:   925938
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2415
- KEGG:   mtu:Rv2350c
- TIGR:   MT2415
- TubercuList:   Rv2350c
- GeneTree:   EBGT00050000017446
- HOGENOM:   HBG565180
- OMA:   KAGISWQ
- ProtClustDB:   CLSK791798
- BRENDA:   3.1.4.3
- GO:   GO:0009405
- InterPro:   IPR007312
- InterPro:   IPR006311
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04185 Phosphoesterase

EC number: =3.1.4.3

Molecular weight: Translated: 55624; Mature: 55493

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCEEEEEEEECCCCHHHHC
GTLSDTRGFDDTTPPVVFAQSGWNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHS
CCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCC
WIGMHNSWNGGANDNWLPAQVPFSPLQGNVPVTMGFYTRRDLPIHYLLADTFTVCDGYFC
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHH
SLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLEDAGVSWKVYQ
HHHCCCCCCEEEEEEEEECCCCCCCCCEEECCCCCHHCCCEEEECCCCCCCCCCEEEEHH
NKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNNRLPKVSWVL
HHHHHHHCCCEEECHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEEC
PGFLLSEHPAFPVNVGAVAIVDALRILLSNPAVWEKTALIVNYDENGGFFDHVVPPTPPP
CCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEECCCCCCCCCC
GTPGEFVTVPDIDSVPGSGGIRGPIGLGFRVPCLVISPYSRGPLMVHDTFDHTSTLKLIR
CCCCCEEECCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEEECCCCHHHHHHHH
ARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQCVPNAVLGT
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC
HHHHCCCEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRRQFFAKAAAATTAGAFMSLAGPIIEKAYGAGPCPGHLTDIEHIVLLMQENRSFDHYF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCEEEEEEEECCCCHHHHC
GTLSDTRGFDDTTPPVVFAQSGWNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHS
CCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCC
WIGMHNSWNGGANDNWLPAQVPFSPLQGNVPVTMGFYTRRDLPIHYLLADTFTVCDGYFC
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHH
SLLGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLEDAGVSWKVYQ
HHHCCCCCCEEEEEEEEECCCCCCCCCEEECCCCCHHCCCEEEECCCCCCCCCCEEEEHH
NKLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADVRNNRLPKVSWVL
HHHHHHHCCCEEECHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEEC
PGFLLSEHPAFPVNVGAVAIVDALRILLSNPAVWEKTALIVNYDENGGFFDHVVPPTPPP
CCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEECCCCCCCCCC
GTPGEFVTVPDIDSVPGSGGIRGPIGLGFRVPCLVISPYSRGPLMVHDTFDHTSTLKLIR
CCCCCEEECCCCCCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEEECCCCHHHHHHHH
ARFGVPVPNLTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQCVPNAVLGT
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VTKTAIPYRVPFPQSMPTQETAPTRGIPSGLC
HHHHCCCEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8757862; 9634230; 12218036