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The map label for this gene is dop [H]

Identifier: 148661927

GI number: 148661927

Start: 2380807

End: 2382471

Strand: Reverse

Name: dop [H]

Synonym: MRA_2127

Alternate gene names: 148661927

Gene position: 2382471-2380807 (Counterclockwise)

Preceding gene: 148661930

Following gene: 148661926

Centisome position: 53.9

GC content: 67.33

Gene sequence:

>1665_bases
ATGTTCTGGGTCGGCGGGCCTTGCTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTCGGCGGGCGTTG
CTTGATGCCCGCCTCCTCAGCGGCCCGCTGCGCGGCCCGCATCGTCGGCGGGCCTAGGCTCTACGGCATGCAGCGGATTA
TCGGAACGGAGGTCGAGTACGGCATTTCCTCGCCGTCGGACCCGACCGCCAACCCGATCCTCACCTCGACGCAGGCGGTG
CTGGCATACGCCGCCGCCGCCGGCATTCAGCGTGCCAAACGCACCCGTTGGGACTACGAGGTGGAATCGCCGCTGCGCGA
CGCCCGGGGCTTCGATTTGAGTCGCTCGGCCGGGCCGCCGCCGGTGGTCGACGCCGACGAGGTCGGCGCGGCCAACATGA
TCCTGACCAACGGGGCGCGGCTGTATGTCGACCACGCGCACCCGGAATACTCCGCGCCCGAATGCACCGACCCGCTGGAC
GCAGTGATCTGGGACAAGGCGGGCGAACGCGTGATGGAGGCCGCTGCCCGCCATGTCGCCAGCGTGCCCGGGGCCGCGAA
ACTGCAGCTGTACAAGAACAACGTCGACGGCAAGGGAGCCTCCTACGGGTCGCACGAGAACTACCTGATGTCGCGGCAGA
CACCGTTCTCGGCGATCATCACCGGGCTGACCCCCTTTCTGGTATCCCGGCAGGTGGTGACCGGCTCGGGCCGGGTCGGC
ATCGGGCCCTCGGGTGATGAGCCCGGCTTCCAGCTATCCCAGCGTTCGGACTACATCGAGGTCGAGGTAGGGCTGGAAAC
AACGCTCAAGCGCGGCATCATCAACACCCGCGACGAACCGCACGCCGACGCCGACAGGTACCGCCGGCTGCACGTCATCA
TCGGCGACGCCAACCTTGCCGAGACGTCGACCTATCTGAAGTTGGGTACCACGGCGCTGGTGCTCGACCTGATCGAAGAA
GGACCAGCCCACGCAATAGATCTGACCGACCTGGCGCTGGCCCGCCCGGTACATGCGGTGCACGCAATCTCCCGCGATCC
GTCGCTGCGAGCGACCGTTGCGCTGGCCGACGGCCGGGAACTGACCGGTCTTGCGCTGCAACGGATCTACCTGGACCGAG
TGGCTAAGTTGGTGGATAGCCGCGACCCGGACCCGCGGGCGGCCGACATCGTGGAAACCTGGGCACACGTGCTGGATCAG
CTCGAGCGTGACCCGATGGATTGCGCGGAGCTGCTGGACTGGCCGGCCAAACTGCGGCTGCTCGACGGTTTCCGGCAGCG
GGAGAACCTGAGCTGGTCGGCGCCCCGGCTGCACCTCGTCGACCTGCAGTACTCCGATGTCCGGCTGGACAAGGGCCTGT
ACAACCGGCTGGTCGCGCGCGGCTCGATGAAGCGTTTAGTCACCGAACACCAGGTGCTGAGTGCGGTGGAGAACCCGCCG
ACCGACACCCGCGCGTATTTCCGCGGCGAATGCCTGCGCCGGTTCGGGGCTGATATCGCCGCGGCTAGCTGGGACTCGGT
GATCTTCGACCTGGGCGGCGACTCGCTGGTTCGCATCCCGACGCTGGAGCCGTTGCGGGGTAGTAAGGCGCATGTTGGTG
CGTTGCTGGATTCGGTGGACAGTGCCGTGGAGCTGGTAGAGCAACTGACCGCTGAGCCTCGCTAA

Upstream 100 bases:

>100_bases
CTGCTCGGGCCGCCGGGGGGCGACGGACGGAAAGGCTCATGCCGGCCTACCCTAGCGCCGGGCCCTGCTTGATTGTCCAC
CTCCTGCGCGGCCCGCTCCG

Downstream 100 bases:

>100_bases
GCCTGGTTAGGCCGGGAAACGTTGGTGTTGACCGGTAGGGTAGAGAAAGACAGCGGGCGTGTGACGCGCACCCGCGGCCT
TTTGAAAGGCCATGAGGAAG

Product: hypothetical protein

Products: NA

Alternate protein names: Deamidase of protein Pup [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAV
LAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLD
AVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVG
IGPSGDEPGFQLSQRSDYIEVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEE
GPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQ
LERDPMDCAELLDWPAKLRLLDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPP
TDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR

Sequences:

>Translated_554_residues
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAV
LAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLD
AVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVG
IGPSGDEPGFQLSQRSDYIEVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEE
GPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQ
LERDPMDCAELLDWPAKLRLLDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPP
TDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR
>Mature_554_residues
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEYGISSPSDPTANPILTSTQAV
LAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLD
AVIWDKAGERVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVG
IGPSGDEPGFQLSQRSDYIEVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLAETSTYLKLGTTALVLDLIEE
GPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQ
LERDPMDCAELLDWPAKLRLLDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPP
TDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVDSAVELVEQLTAEPR

Specific function: Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022366
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 60130; Mature: 60130

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEY
CCEECCCCCCCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHC
GISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHCCCCCCCCCCCC
PVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVA
CCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVG
HCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCC
IGPSGDEPGFQLSQRSDYIEVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLA
CCCCCCCCCCEECCCCCEEEEEECHHHHHHHCCCCCCCCCCCCHHHCEEEEEEEECCCCH
ETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRE
HHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
LTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHH
LDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPP
HHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVD
CCHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHH
SAVELVEQLTAEPR
HHHHHHHHHHCCCC
>Mature Secondary Structure
MFWVGGPCLMPASSAARCAARIVGGRCLMPASSAARCAARIVGGPRLYGMQRIIGTEVEY
CCEECCCCCCCCCHHHHHHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHC
GISSPSDPTANPILTSTQAVLAYAAAAGIQRAKRTRWDYEVESPLRDARGFDLSRSAGPP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHCCCCCCCCCCCC
PVVDADEVGAANMILTNGARLYVDHAHPEYSAPECTDPLDAVIWDKAGERVMEAAARHVA
CCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAIITGLTPFLVSRQVVTGSGRVG
HCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCC
IGPSGDEPGFQLSQRSDYIEVEVGLETTLKRGIINTRDEPHADADRYRRLHVIIGDANLA
CCCCCCCCCCEECCCCCEEEEEECHHHHHHHCCCCCCCCCCCCHHHCEEEEEEEECCCCH
ETSTYLKLGTTALVLDLIEEGPAHAIDLTDLALARPVHAVHAISRDPSLRATVALADGRE
HHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
LTGLALQRIYLDRVAKLVDSRDPDPRAADIVETWAHVLDQLERDPMDCAELLDWPAKLRL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHH
LDGFRQRENLSWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSMKRLVTEHQVLSAVENPP
HHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPTLEPLRGSKAHVGALLDSVD
CCHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHH
SAVELVEQLTAEPR
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]