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The map label for this gene is treZ

Identifier: 148661358

GI number: 148661358

Start: 1766917

End: 1768659

Strand: Reverse

Name: treZ

Synonym: MRA_1574

Alternate gene names: 148661358

Gene position: 1768659-1766917 (Counterclockwise)

Preceding gene: 148661359

Following gene: 148661344

Centisome position: 40.02

GC content: 65.17

Gene sequence:

>1743_bases
ATGCCTGAATTCCGAGTATGGGCACCCAAACCCGCGCTGGTCCGGCTCGACGTGAACGGTGCGGTACATGCGATGACCCG
CTCAGCCGACGGCTGGTGGCACACGACGGTGGCGGCGCCGGCGGACGCCCGCTACGGATACCTACTCGACGACGACCCCA
CCGTGCTACCCGACCCGCGATCGGCCCGCCAACCCGACGGGGTGCACGCACGCTCCCAGCGGTGGGAGCCGCCCGGCCAG
TTTGGTGCTGCCCGGACCGACACTGGCTGGCCGGGGCGGTCGGTCGAGGGCGCGGTGATCTACGAGTTGCACATCGGCAC
GTTCACCACGGCTGGCACGTTCGACGCCGCCATCGAAAAGCTGGATTACCTGGTCGATCTCGGAATCGACTTCGTCGAGC
TGATGCCGGTCAATTCCTTTGCCGGAACCCGCGGTTGGGGATATGACGGTGTGCTGTGGTACAGCGTGCACGAACCCTAC
GGCGGCCCCGACGGTCTGGTCCGGTTCATCGACGCATGCCATGCCCGTCGTTTGGGCGTGTTGATCGACGCGGTGTTCAA
CCATCTCGGCCCATCGGGCAACTACCTGCCGCGATTCGGCCCGTACCTGTCGTCGGCCAGCAACCCGTGGGGGGACGGCA
TCAATATCGCCGGCGCCGACTCCGACGAGGTTCGCCACTATATCATCGACTGCGCGCTGCGATGGATGCGCGACTTCCAC
GCCGACGGCTTGCGGTTGGACGCCGTGCATGCACTGGTGGACACCACCGCCGTGCATGTCCTCGAGGAGCTTGCCAACGC
GACCCGCTGGCTGTCAGGCCAGTTGGGCCGTCCGTTGTCGCTGATCGCCGAAACCGACCGAAACGACCCGCGGCTGATCA
CCCGGCCCAGCCATGGCGGCTACGGGATCACCGCCCAATGGAATGACGACATCCATCACGCCATCCATACCGCGGTATCC
GGTGAGCGGCAAGGCTACTATGCGGATTTCGGCTCGCTGGCCACTCTCGCGTACACGCTGCGCAACGGCTACTTCCACGC
CGGCACGTATTCGTCGTTCCGGCGTCGTCGGCACGGGCGTGCATTGGACACTTCTGCGATCCCGGCCACCAGGCTGCTCG
CCTACACCTGCACCCACGACCAAGTCGGCAACCGCGCTCTCGGCGACCGCCCATCGCAATACCTGACCGGTGGCCAGCTC
GCGATCAAGGCGGCGTTGACCCTTGGATCTCCCTATACCGCAATGCTTTTCATGGGCGAAGAGTGGGGGGCCTCCAGCCC
GTTTCAGTTCTTTTGCTCACATCCCGAACCGGAACTGGCCCATAGCACCGTGGCAGGACGCAAGGAGGAGTTCGCCGAAC
ATGGCTGGGCCGCGGACGACATTCCGGACCCGCAGGACCCGCAGACATTCCAGCGCTGCAAGCTGAACTGGGCCGAAGCC
GGCTCCGGCGAACACGCCAGGTTGCACCGGTTTTATCGCGATCTGATCGCTTTGCGGCACAACGAGGCCGACCTTGCGGA
CCCCTGGCTGGACCACCTTATGGTCGACTACGACGAGCAGCAACGCTGGGTTGTGATGCGCCGCGGCCAGCTGATGATCG
CCTGCAACCTTGGTGCCGAGCCAACCTGCGTGCCGGTCAGCGGTGAGCTGGTGCTGGCCTGGGAGTCGCCGATTATCGGC
GATAATTCCACCGAGCTAGCAGCGTATTCGCTTGCGATACTGCGGGCTGCGGAGCCGGCCTGA

Upstream 100 bases:

>100_bases
GGGTCGTGGACAGATGCCCTCACCGGATTCACGGCCAGTGGGCACACGCCGGCCGTCGAGCTGTTCGCGGATCTGCCTGT
GGTGCTGCTGGTGCGCGACA

Downstream 100 bases:

>100_bases
TGCTGGGGCGGCATGCTCACCGAAACGATGCGGCCTGCAGGTTGGTAATCCGGGCAATCACATCAAAGTCGGCGTCACGG
TGGACGATACGCGCACCGTG

Product: maltooligosyl trehalose trehalohydrolase

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MPEFRVWAPKPALVRLDVNGAVHAMTRSADGWWHTTVAAPADARYGYLLDDDPTVLPDPRSARQPDGVHARSQRWEPPGQ
FGAARTDTGWPGRSVEGAVIYELHIGTFTTAGTFDAAIEKLDYLVDLGIDFVELMPVNSFAGTRGWGYDGVLWYSVHEPY
GGPDGLVRFIDACHARRLGVLIDAVFNHLGPSGNYLPRFGPYLSSASNPWGDGINIAGADSDEVRHYIIDCALRWMRDFH
ADGLRLDAVHALVDTTAVHVLEELANATRWLSGQLGRPLSLIAETDRNDPRLITRPSHGGYGITAQWNDDIHHAIHTAVS
GERQGYYADFGSLATLAYTLRNGYFHAGTYSSFRRRRHGRALDTSAIPATRLLAYTCTHDQVGNRALGDRPSQYLTGGQL
AIKAALTLGSPYTAMLFMGEEWGASSPFQFFCSHPEPELAHSTVAGRKEEFAEHGWAADDIPDPQDPQTFQRCKLNWAEA
GSGEHARLHRFYRDLIALRHNEADLADPWLDHLMVDYDEQQRWVVMRRGQLMIACNLGAEPTCVPVSGELVLAWESPIIG
DNSTELAAYSLAILRAAEPA

Sequences:

>Translated_580_residues
MPEFRVWAPKPALVRLDVNGAVHAMTRSADGWWHTTVAAPADARYGYLLDDDPTVLPDPRSARQPDGVHARSQRWEPPGQ
FGAARTDTGWPGRSVEGAVIYELHIGTFTTAGTFDAAIEKLDYLVDLGIDFVELMPVNSFAGTRGWGYDGVLWYSVHEPY
GGPDGLVRFIDACHARRLGVLIDAVFNHLGPSGNYLPRFGPYLSSASNPWGDGINIAGADSDEVRHYIIDCALRWMRDFH
ADGLRLDAVHALVDTTAVHVLEELANATRWLSGQLGRPLSLIAETDRNDPRLITRPSHGGYGITAQWNDDIHHAIHTAVS
GERQGYYADFGSLATLAYTLRNGYFHAGTYSSFRRRRHGRALDTSAIPATRLLAYTCTHDQVGNRALGDRPSQYLTGGQL
AIKAALTLGSPYTAMLFMGEEWGASSPFQFFCSHPEPELAHSTVAGRKEEFAEHGWAADDIPDPQDPQTFQRCKLNWAEA
GSGEHARLHRFYRDLIALRHNEADLADPWLDHLMVDYDEQQRWVVMRRGQLMIACNLGAEPTCVPVSGELVLAWESPIIG
DNSTELAAYSLAILRAAEPA
>Mature_579_residues
PEFRVWAPKPALVRLDVNGAVHAMTRSADGWWHTTVAAPADARYGYLLDDDPTVLPDPRSARQPDGVHARSQRWEPPGQF
GAARTDTGWPGRSVEGAVIYELHIGTFTTAGTFDAAIEKLDYLVDLGIDFVELMPVNSFAGTRGWGYDGVLWYSVHEPYG
GPDGLVRFIDACHARRLGVLIDAVFNHLGPSGNYLPRFGPYLSSASNPWGDGINIAGADSDEVRHYIIDCALRWMRDFHA
DGLRLDAVHALVDTTAVHVLEELANATRWLSGQLGRPLSLIAETDRNDPRLITRPSHGGYGITAQWNDDIHHAIHTAVSG
ERQGYYADFGSLATLAYTLRNGYFHAGTYSSFRRRRHGRALDTSAIPATRLLAYTCTHDQVGNRALGDRPSQYLTGGQLA
IKAALTLGSPYTAMLFMGEEWGASSPFQFFCSHPEPELAHSTVAGRKEEFAEHGWAADDIPDPQDPQTFQRCKLNWAEAG
SGEHARLHRFYRDLIALRHNEADLADPWLDHLMVDYDEQQRWVVMRRGQLMIACNLGAEPTCVPVSGELVLAWESPIIGD
NSTELAAYSLAILRAAEPA

Specific function: Catalyzes The Formation Of The Alpha-1,6-Glucosidic Linkages In Glycogen By Scission Of A 1,4-Alpha-Linked Oligosaccharide From Growing Alpha-1,4-Glucan Chains And The Subsequent Attachment Of The Oligosaccharide To The Alpha-1,6 Position. Has A Preferenc

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI1789839, Length=242, Percent_Identity=28.5123966942149, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TREZ_MYCTU (Q10769)

Other databases:

- EMBL:   BX842577
- EMBL:   AE000516
- PIR:   G70763
- RefSeq:   NP_336066.1
- RefSeq:   YP_177819.1
- ProteinModelPortal:   Q10769
- SMR:   Q10769
- EnsemblBacteria:   EBMYCT00000002030
- EnsemblBacteria:   EBMYCT00000072604
- GeneID:   886355
- GeneID:   924321
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1613
- KEGG:   mtu:Rv1562c
- TIGR:   MT1613
- TubercuList:   Rv1562c
- GeneTree:   EBGT00050000016657
- HOGENOM:   HBG367595
- OMA:   TPMLFMG
- ProtClustDB:   CLSK799816
- BRENDA:   3.2.1.141
- GO:   GO:0005737
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR022567
- InterPro:   IPR012768
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- PIRSF:   PIRSF006337
- TIGRFAMs:   TIGR02402

Pfam domain/function: PF00128 Alpha-amylase; PF11941 DUF3459; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =3.2.1.141

Molecular weight: Translated: 64077; Mature: 63946

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: ACT_SITE 247-247 ACT_SITE 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEFRVWAPKPALVRLDVNGAVHAMTRSADGWWHTTVAAPADARYGYLLDDDPTVLPDPR
CCCCEEECCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCEEECCCCCCCCCCC
SARQPDGVHARSQRWEPPGQFGAARTDTGWPGRSVEGAVIYELHIGTFTTAGTFDAAIEK
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCHHHHHHHH
LDYLVDLGIDFVELMPVNSFAGTRGWGYDGVLWYSVHEPYGGPDGLVRFIDACHARRLGV
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LIDAVFNHLGPSGNYLPRFGPYLSSASNPWGDGINIAGADSDEVRHYIIDCALRWMRDFH
HHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHC
ADGLRLDAVHALVDTTAVHVLEELANATRWLSGQLGRPLSLIAETDRNDPRLITRPSHGG
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCC
YGITAQWNDDIHHAIHTAVSGERQGYYADFGSLATLAYTLRNGYFHAGTYSSFRRRRHGR
EEEEEECCCHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHCCC
ALDTSAIPATRLLAYTCTHDQVGNRALGDRPSQYLTGGQLAIKAALTLGSPYTAMLFMGE
CCCCCCCCHHHEEEEEECCHHCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCEEEEEECC
EWGASSPFQFFCSHPEPELAHSTVAGRKEEFAEHGWAADDIPDPQDPQTFQRCKLNWAEA
CCCCCCCHHHEECCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHCC
GSGEHARLHRFYRDLIALRHNEADLADPWLDHLMVDYDEQQRWVVMRRGQLMIACNLGAE
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCEEEEEECCCEEEEEECCCC
PTCVPVSGELVLAWESPIIGDNSTELAAYSLAILRAAEPA
CEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PEFRVWAPKPALVRLDVNGAVHAMTRSADGWWHTTVAAPADARYGYLLDDDPTVLPDPR
CCCEEECCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCEEECCCCCCCCCCC
SARQPDGVHARSQRWEPPGQFGAARTDTGWPGRSVEGAVIYELHIGTFTTAGTFDAAIEK
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCHHHHHHHH
LDYLVDLGIDFVELMPVNSFAGTRGWGYDGVLWYSVHEPYGGPDGLVRFIDACHARRLGV
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LIDAVFNHLGPSGNYLPRFGPYLSSASNPWGDGINIAGADSDEVRHYIIDCALRWMRDFH
HHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHC
ADGLRLDAVHALVDTTAVHVLEELANATRWLSGQLGRPLSLIAETDRNDPRLITRPSHGG
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCC
YGITAQWNDDIHHAIHTAVSGERQGYYADFGSLATLAYTLRNGYFHAGTYSSFRRRRHGR
EEEEEECCCHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHCCC
ALDTSAIPATRLLAYTCTHDQVGNRALGDRPSQYLTGGQLAIKAALTLGSPYTAMLFMGE
CCCCCCCCHHHEEEEEECCHHCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCEEEEEECC
EWGASSPFQFFCSHPEPELAHSTVAGRKEEFAEHGWAADDIPDPQDPQTFQRCKLNWAEA
CCCCCCCHHHEECCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHCC
GSGEHARLHRFYRDLIALRHNEADLADPWLDHLMVDYDEQQRWVVMRRGQLMIACNLGAE
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCEEEEEECCCEEEEEECCCC
PTCVPVSGELVLAWESPIIGDNSTELAAYSLAILRAAEPA
CEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036