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The map label for this gene is rffA [H]

Identifier: 148661298

GI number: 148661298

Start: 1695513

End: 1695953

Strand: Reverse

Name: rffA [H]

Synonym: MRA_1514

Alternate gene names: 148661298

Gene position: 1695953-1695513 (Counterclockwise)

Preceding gene: 148661299

Following gene: 148661293

Centisome position: 38.37

GC content: 60.54

Gene sequence:

>441_bases
TTGCCCAGCGAATTAGTCGCTGCCTTTCTATGGGCTCAGTTCGAGGAAGCCGAGCGGATCACGCGTATCCGATTGGACCT
ATGGAACCGGTATCATGAAAGCTTCGAATCATTGGAACAGCGGGGGCTCCTGCGCCGTCCGATCATCCCACAGGGCTGCT
CTCACAACGCCCACATGTACTACGTGTTACTAGCGCCCAGCGCCGATCGGGAGGAGGTGCTGGCGCGTCTGACGAGCGAA
GGTATAGGCGCGGTCTTTCATTACGTGCCGCTTCACGATTCGCCGGCCGGGCGTCGCTACGGCCGCACCAATGGCAACCT
GACCGTCACCAACGACGTGGCTTCCCGCCTAATCCGTCTGCCGATGTGGGTTGGACTTCAGGAGGTAGATCAGAGCCGCG
TTGTCGAGGCGCTCACGCGCATCCTGACGTTGCGCGCATGA

Upstream 100 bases:

>100_bases
GCTCCGGGCAGAGATTCTCAGGGAAAAGGGCACCAATCGCAGCCGCTTCCTTCGCAACGAGGTCGACAAATATACGTGGC
AGGACAAAGGGTCTTCCTAT

Downstream 100 bases:

>100_bases
AGATAAGCAGTGCGAAGGATACAATGAAGCCCTTAAAGGGCCTCGACGAGCAGTGAGTGATCGCGGCGACGACGATAATC
CGGGAAATCATCGCTACCGC

Product: hypothetical protein

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MPSELVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMYYVLLAPSADREEVLARLTSE
GIGAVFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRLPMWVGLQEVDQSRVVEALTRILTLRA

Sequences:

>Translated_146_residues
MPSELVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMYYVLLAPSADREEVLARLTSE
GIGAVFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRLPMWVGLQEVDQSRVVEALTRILTLRA
>Mature_145_residues
PSELVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMYYVLLAPSADREEVLARLTSEG
IGAVFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRLPMWVGLQEVDQSRVVEALTRILTLRA

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=136, Percent_Identity=42.6470588235294, Blast_Score=120, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 16739; Mature: 16608

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSELVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
YVLLAPSADREEVLARLTSEGIGAVFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRL
EEEECCCCCHHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
PMWVGLQEVDQSRVVEALTRILTLRA
HHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSELVAAFLWAQFEEAERITRIRLDLWNRYHESFESLEQRGLLRRPIIPQGCSHNAHMY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
YVLLAPSADREEVLARLTSEGIGAVFHYVPLHDSPAGRRYGRTNGNLTVTNDVASRLIRL
EEEECCCCCHHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
PMWVGLQEVDQSRVVEALTRILTLRA
HHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]