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The map label for this gene is rffA [H]

Identifier: 148661299

GI number: 148661299

Start: 1696062

End: 1696661

Strand: Reverse

Name: rffA [H]

Synonym: MRA_1515

Alternate gene names: 148661299

Gene position: 1696661-1696062 (Counterclockwise)

Preceding gene: 148661300

Following gene: 148661298

Centisome position: 38.39

GC content: 60.83

Gene sequence:

>600_bases
GTGTCAGATCACAAAGTTCCATTCAATCGGCCGTACATGACCGGCCGGGAGCTCGCGTACATCGCCGAAGCGCATTCTTG
CGGCCACCTGGCCGGCGATGGTCCCTTCACCCGGCGTAGCCATGCATGGCTCGAACAGCAGACCGGTTGTCGCAAGGCAC
TACTCACCCCCTCATGCACCGCCGCGCTGGAAATGATGGCGCTGCTTCTGGACATAGAGGAGGGGGACGAAGTCATACTG
CCGTCATACACCTTTGTCTCTACCGCCAACGCCTTCGTGTTGCGCGGTGGTGTGCCAGTCTTTGTCGATATTCGGCCCGA
CACGCTCAACATTGATGAAACTCGCATCGTAGACGCCATCACCCCGCGAACCAAGGCCATCGTCCCCGTTCACTATGCCG
GCGTGGCCTGCGAGATGGACGCGATCATGAAGATCGCCACGCACCACAACCTGGCGGTGGTCGAAGACGCGGCCCAAGGC
GCGATGGCGTCGTATCGTGGGCGGGCGCTCGGCAGCATCGGCGACCTGGGAGCGCTCTCATTTCACGAGACCAAGAATGT
GATTTCCGGCGAAGGCGGCGCCCTGCTTGTCAACTCATAA

Upstream 100 bases:

>100_bases
CTAGGGATCCGCCGGCCACAAGGAGAATTCTGGTGGATGTTCGACCCCGATCACCGAACACTTCAGCGCCCTGTTCGATG
ATTTGAGAAGTTGGCGTCGA

Downstream 100 bases:

>100_bases
GACTTCCTGCTCCGGGCAGAGATTCTCAGGGAAAAGGGCACCAATCGCAGCCGCTTCCTTCGCAACGAGGTCGACAAATA
TACGTGGCAGGACAAAGGGT

Product: hypothetical protein

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCTAALEMMALLLDIEEGDEVIL
PSYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAITPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQG
AMASYRGRALGSIGDLGALSFHETKNVISGEGGALLVNS

Sequences:

>Translated_199_residues
MSDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCTAALEMMALLLDIEEGDEVIL
PSYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAITPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQG
AMASYRGRALGSIGDLGALSFHETKNVISGEGGALLVNS
>Mature_198_residues
SDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCTAALEMMALLLDIEEGDEVILP
SYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAITPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQGA
MASYRGRALGSIGDLGALSFHETKNVISGEGGALLVNS

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=194, Percent_Identity=68.0412371134021, Blast_Score=270, Evalue=6e-74,
Organism=Escherichia coli, GI145693159, Length=194, Percent_Identity=41.7525773195876, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 21350; Mature: 21218

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCHH
AALEMMALLLDIEEGDEVILPSYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAI
HHHHHHHHHHCCCCCCCEEECCCCEEECCCEEEEECCCEEEEEECCCCCCCCHHEEEECC
TPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQGAMASYRGRALGSIGDLGALS
CCCCCEEEEEEECCEEECHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCEE
FHETKNVISGEGGALLVNS
HHHHCHHCCCCCCEEEEEC
>Mature Secondary Structure 
SDHKVPFNRPYMTGRELAYIAEAHSCGHLAGDGPFTRRSHAWLEQQTGCRKALLTPSCT
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCHH
AALEMMALLLDIEEGDEVILPSYTFVSTANAFVLRGGVPVFVDIRPDTLNIDETRIVDAI
HHHHHHHHHHCCCCCCCEEECCCCEEECCCEEEEECCCEEEEEECCCCCCCCHHEEEECC
TPRTKAIVPVHYAGVACEMDAIMKIATHHNLAVVEDAAQGAMASYRGRALGSIGDLGALS
CCCCCEEEEEEECCEEECHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCEE
FHETKNVISGEGGALLVNS
HHHHCHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]