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The map label for this gene is mscL

Identifier: 148660766

GI number: 148660766

Start: 1102333

End: 1102788

Strand: Reverse

Name: mscL

Synonym: MRA_0992

Alternate gene names: 148660766

Gene position: 1102788-1102333 (Counterclockwise)

Preceding gene: 148660770

Following gene: 148660761

Centisome position: 24.95

GC content: 60.09

Gene sequence:

>456_bases
ATGCTCAAAGGATTCAAGGAGTTTCTCGCGCGGGGTAATATCGTCGACCTGGCTGTCGCGGTGGTAATCGGCACAGCGTT
CACGGCGTTGGTCACCAAGTTCACCGACAGCATCATTACGCCGCTGATCAACCGGATCGGCGTCAACGCACAGTCCGACG
TCGGCATCTTGCGGATCGGTATCGGCGGTGGTCAGACCATTGACTTGAACGTCTTGTTGTCGGCAGCGATCAACTTTTTC
CTGATCGCGTTCGCGGTGTACTTCCTAGTCGTGCTGCCCTACAACACACTACGCAAGAAGGGGGAGGTCGAGCAGCCGGG
CGACACCCAAGTCGTGCTGCTCACCGAAATCCGCGATCTGCTCGCGCAAACGAACGGGGACTCGCCGGGGAGGCACGGCG
GCCGTGGGACACCATCGCCAACCGACGGGCCTCGCGCGAGCACAGAATCGCAATAG

Upstream 100 bases:

>100_bases
ACGGCGATCCCAGCGTCGTGGCCATTGACACCACCTGGTCTAGTTTTTCTAGGTGTTCCACTCGCGGACTGCCGGCCGCC
GACTGCAGAAAGGACATCGC

Downstream 100 bases:

>100_bases
CCCGACACTCGATCCGGATTCAGATCTCCAAGCTCGACAACTGCCCGATGATCTGTGCCGCTAGCGGATTCAGCGTCGCC
ATTCCATCGCGCACCGCATA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MLKGFKEFLARGNIVDLAVAVVIGTAFTALVTKFTDSIITPLINRIGVNAQSDVGILRIGIGGGQTIDLNVLLSAAINFF
LIAFAVYFLVVLPYNTLRKKGEVEQPGDTQVVLLTEIRDLLAQTNGDSPGRHGGRGTPSPTDGPRASTESQ

Sequences:

>Translated_151_residues
MLKGFKEFLARGNIVDLAVAVVIGTAFTALVTKFTDSIITPLINRIGVNAQSDVGILRIGIGGGQTIDLNVLLSAAINFF
LIAFAVYFLVVLPYNTLRKKGEVEQPGDTQVVLLTEIRDLLAQTNGDSPGRHGGRGTPSPTDGPRASTESQ
>Mature_151_residues
MLKGFKEFLARGNIVDLAVAVVIGTAFTALVTKFTDSIITPLINRIGVNAQSDVGILRIGIGGGQTIDLNVLLSAAINFF
LIAFAVYFLVVLPYNTLRKKGEVEQPGDTQVVLLTEIRDLLAQTNGDSPGRHGGRGTPSPTDGPRASTESQ

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=134, Percent_Identity=36.5671641791045, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_MYCBO (P0A5K9)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854668.1
- ProteinModelPortal:   P0A5K9
- SMR:   P0A5K9
- EnsemblBacteria:   EBMYCT00000017957
- GeneID:   1093319
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1011c
- GeneTree:   EBGT00050000017393
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK00567
- BioCyc:   MBOV233413:MB1011C-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089
- Gene3D:   G3DSA:1.20.5.220
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16024; Mature: 16024

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1d82ca40)-; HASH(0x1d4e67c4)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGFKEFLARGNIVDLAVAVVIGTAFTALVTKFTDSIITPLINRIGVNAQSDVGILRIG
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IGGGQTIDLNVLLSAAINFFLIAFAVYFLVVLPYNTLRKKGEVEQPGDTQVVLLTEIRDL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCEEEEHHHHHHH
LAQTNGDSPGRHGGRGTPSPTDGPRASTESQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MLKGFKEFLARGNIVDLAVAVVIGTAFTALVTKFTDSIITPLINRIGVNAQSDVGILRIG
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IGGGQTIDLNVLLSAAINFFLIAFAVYFLVVLPYNTLRKKGEVEQPGDTQVVLLTEIRDL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCEEEEHHHHHHH
LAQTNGDSPGRHGGRGTPSPTDGPRASTESQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972