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The map label for this gene is pucA [H]

Identifier: 148660142

GI number: 148660142

Start: 454593

End: 455735

Strand: Reverse

Name: pucA [H]

Synonym: MRA_0385

Alternate gene names: 148660142

Gene position: 455735-454593 (Counterclockwise)

Preceding gene: 148660146

Following gene: 148660141

Centisome position: 10.31

GC content: 69.55

Gene sequence:

>1143_bases
ATGGCGATCTGGGCCGCCGGTGACACCGCGGGGGTCGCGACGGTGGTGCGAACCTTGCGGTCAGCGCCGCGGCCCCCAGG
GGCCGCGATGGTGGTAGCCCCGGACGGTTCGGTAAGTGGGTCGGTGTCGGGCGGCTGCGTGGAGGGTGCGGTATATGAGC
TGGCCGCCGAGGTGGCCCAAACCGGGATACCGCGGCTGGAACACTACGGAGTCAGCGACGATACCGCATTCGCGGTAGGC
CTGACCTGTGGCGGCATCATCGACGTCTTCGTCGAGCCCGTATCGCGGGCCACGTTTCCCGAGCTGGGCGAGCTGGCCGA
CGACATCGGCGCGCAACGCCCGGTCGCCATCGCGACCGTCATCGCCCATCCGGACGAGCGGCGGGTGGGTCGCCGACTGG
TGATCCGGCCCGACACAAAGAGCCCCGTGACGGGTTCGCTGGGTTCGGCGCGTGCCGATGCGGCGGTTATCGACGATGCG
CGGGGTCTGCTCGCGGTGGGCCGCAGCGAGATCCTCGAATATGGGCCCGATGGGCAGCGGCGTGGCGAGGGCATGGAGGT
CTTCGTGTCCAGCCACGCGCCGCGCCCGCGGATGCTGGTGTTCGGTGCCATCGACTTCGCCGCCGCGCTGGCGCGGCAGG
GTTCGTTCCTCGGCTACCGGGTCACCGTCTGCGACGCCCGCGCGGTATTCGCCACGCCAGCGCGCTTTCCGACGGCCGAT
GACGTCGTCGTCGCATGGCCCCACCGCTATCTGGCTGCCCAGGCGGAGGCGGGTGGTATCGACGAGCGCACGGTGATCTG
CGTGCTCACCCACGATCCGAAGTTCGACGTCCCGGTGCTCGAGGTGGCGCTGCGCCTGGGTGTCGGATACGTGGGGGCGA
TGGGATCACGCAAGACGCACGACGACCGGATGGATCGGCTACGCGCGGCGGGCCTGACCGACGCCGAGCTGAGCCGGTTG
TCCAGCCCGATCGGGCTGGATCTCGGTGCCCGAACCCCCGAGGAGACCGCGGTCTCGATCGCGGCCGACATCATCGCCCG
ACGTTGGGGCGGAGGGGGACGCCCGCTGGCCGATATTGCCGGACGGATCCACCATGATGCGCAGGTAGCGGGCGAGTTCA
AGGATTACTTAACTCGACATTGA

Upstream 100 bases:

>100_bases
CCGAGCCCAGGCGAACCACCGCCGAATAGGCCCGAAGTTGAGCCGGGGTCATGCACTGAAGCCTACTTTGGGAAGCGTGC
GTGAAGTGCTTGCCGAGCTG

Downstream 100 bases:

>100_bases
CGCCCTTTTCGCGGGCGGCCGACACTGGGGCGCATGCAAGTACCTGGGCCTTTCGAATACGAACGTGCCACCAGCGTGGA
CCACGCCATCGGATTGTTGG

Product: hypothetical protein

Products: NA

Alternate protein names: XDHase subunit A [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MAIWAAGDTAGVATVVRTLRSAPRPPGAAMVVAPDGSVSGSVSGGCVEGAVYELAAEVAQTGIPRLEHYGVSDDTAFAVG
LTCGGIIDVFVEPVSRATFPELGELADDIGAQRPVAIATVIAHPDERRVGRRLVIRPDTKSPVTGSLGSARADAAVIDDA
RGLLAVGRSEILEYGPDGQRRGEGMEVFVSSHAPRPRMLVFGAIDFAAALARQGSFLGYRVTVCDARAVFATPARFPTAD
DVVVAWPHRYLAAQAEAGGIDERTVICVLTHDPKFDVPVLEVALRLGVGYVGAMGSRKTHDDRMDRLRAAGLTDAELSRL
SSPIGLDLGARTPEETAVSIAADIIARRWGGGGRPLADIAGRIHHDAQVAGEFKDYLTRH

Sequences:

>Translated_380_residues
MAIWAAGDTAGVATVVRTLRSAPRPPGAAMVVAPDGSVSGSVSGGCVEGAVYELAAEVAQTGIPRLEHYGVSDDTAFAVG
LTCGGIIDVFVEPVSRATFPELGELADDIGAQRPVAIATVIAHPDERRVGRRLVIRPDTKSPVTGSLGSARADAAVIDDA
RGLLAVGRSEILEYGPDGQRRGEGMEVFVSSHAPRPRMLVFGAIDFAAALARQGSFLGYRVTVCDARAVFATPARFPTAD
DVVVAWPHRYLAAQAEAGGIDERTVICVLTHDPKFDVPVLEVALRLGVGYVGAMGSRKTHDDRMDRLRAAGLTDAELSRL
SSPIGLDLGARTPEETAVSIAADIIARRWGGGGRPLADIAGRIHHDAQVAGEFKDYLTRH
>Mature_379_residues
AIWAAGDTAGVATVVRTLRSAPRPPGAAMVVAPDGSVSGSVSGGCVEGAVYELAAEVAQTGIPRLEHYGVSDDTAFAVGL
TCGGIIDVFVEPVSRATFPELGELADDIGAQRPVAIATVIAHPDERRVGRRLVIRPDTKSPVTGSLGSARADAAVIDDAR
GLLAVGRSEILEYGPDGQRRGEGMEVFVSSHAPRPRMLVFGAIDFAAALARQGSFLGYRVTVCDARAVFATPARFPTADD
VVVAWPHRYLAAQAEAGGIDERTVICVLTHDPKFDVPVLEVALRLGVGYVGAMGSRKTHDDRMDRLRAAGLTDAELSRLS
SPIGLDLGARTPEETAVSIAADIIARRWGGGGRPLADIAGRIHHDAQVAGEFKDYLTRH

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function [H]

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789240, Length=243, Percent_Identity=25.9259259259259, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003777 [H]

Pfam domain/function: PF02625 XdhC_CoxI [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 39899; Mature: 39767

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIWAAGDTAGVATVVRTLRSAPRPPGAAMVVAPDGSVSGSVSGGCVEGAVYELAAEVAQ
CEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
TGIPRLEHYGVSDDTAFAVGLTCGGIIDVFVEPVSRATFPELGELADDIGAQRPVAIATV
CCCCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEE
IAHPDERRVGRRLVIRPDTKSPVTGSLGSARADAAVIDDARGLLAVGRSEILEYGPDGQR
EECCCHHHHCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHCHHHHHHCCCCCCC
RGEGMEVFVSSHAPRPRMLVFGAIDFAAALARQGSFLGYRVTVCDARAVFATPARFPTAD
CCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCC
DVVVAWPHRYLAAQAEAGGIDERTVICVLTHDPKFDVPVLEVALRLGVGYVGAMGSRKTH
CEEEECCHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHCCCCCCCCC
DDRMDRLRAAGLTDAELSRLSSPIGLDLGARTPEETAVSIAADIIARRWGGGGRPLADIA
HHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
GRIHHDAQVAGEFKDYLTRH
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIWAAGDTAGVATVVRTLRSAPRPPGAAMVVAPDGSVSGSVSGGCVEGAVYELAAEVAQ
EEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
TGIPRLEHYGVSDDTAFAVGLTCGGIIDVFVEPVSRATFPELGELADDIGAQRPVAIATV
CCCCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEE
IAHPDERRVGRRLVIRPDTKSPVTGSLGSARADAAVIDDARGLLAVGRSEILEYGPDGQR
EECCCHHHHCCEEEECCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHCHHHHHHCCCCCCC
RGEGMEVFVSSHAPRPRMLVFGAIDFAAALARQGSFLGYRVTVCDARAVFATPARFPTAD
CCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCC
DVVVAWPHRYLAAQAEAGGIDERTVICVLTHDPKFDVPVLEVALRLGVGYVGAMGSRKTH
CEEEECCHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHCCCCCCCCC
DDRMDRLRAAGLTDAELSRLSSPIGLDLGARTPEETAVSIAADIIARRWGGGGRPLADIA
HHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
GRIHHDAQVAGEFKDYLTRH
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]