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The map label for this gene is trmH [H]

Identifier: 148660146

GI number: 148660146

Start: 457631

End: 458293

Strand: Reverse

Name: trmH [H]

Synonym: MRA_0388

Alternate gene names: 148660146

Gene position: 458293-457631 (Counterclockwise)

Preceding gene: 148660147

Following gene: 148660142

Centisome position: 10.37

GC content: 69.83

Gene sequence:

>663_bases
GTGGACCTCGCGATGAGTGCGCTTGGGCCCGGGCCTACGGAATGGGGCGCGCCGACCGGCGGCGTGGGGCCCTGGGCAGG
CGACCTCCCGGACGACCCCCGATACGACCCGGTGTTGTTGCGCGACGGCGATGCTCGCAACGTCGTCGACGCCTACCGGT
ACTGGACCCGAGAGGCGATCATCGCCGACATCGATACGCGCCGTCACCCCTTGCACGTGGCGATCGAGAACTTCGGACAC
GATGCCAATATCGGCTCGGTGGTGCGCACCGCCAATGCATTCGCCGTGCACACCGTGCACATCGTCGGGCGTCGGCGGTG
GAATCGGCGCGGCGCCATGGTGACCGACCGCTATCAGCGGTTATGCCACCACGACAGCACCACCGGGCTGCTGGAGTTCG
CGGCGGGCGCCGGCTTGACCGTGGTGGCGGTGGACAACGTCCCGGGTGCGGCGCGCCTGGAGCAGACCGCGTTGCCGCGG
GAATGCCTGCTGTTATTCGGCCAGGAAGGGCCCGGCATTACCGACGACGCCCGTGCCGGCGCGGCGGTCACGGTGTCGAT
CGCCCAGTTCGGGTCGACGCGAAGCATTAACGCCGGCGTGGCCGCCGGAATCGCGATGCACGCCTGGATACGGCAGCACG
CGGACCTGGGCCGCGCCTGGTAG

Upstream 100 bases:

>100_bases
TGGACGCGGTCCACATTAGCGCGTCGCTGTGTACGCAGGGCGAGATCAACGAGGCGTTGCTGTACGCGACGGTCGCGACA
GTGGGAACCCACGCCGCGTT

Downstream 100 bases:

>100_bases
GCCTCGGCGAGCAGACACAAAATCGCACGAATCAACCGCAAACCGTGCGATTTTGCGTCTGCTCGCCGGCCCGTGCTAGC
GCGGTTGCGCCGGCCTCATC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MDLAMSALGPGPTEWGAPTGGVGPWAGDLPDDPRYDPVLLRDGDARNVVDAYRYWTREAIIADIDTRRHPLHVAIENFGH
DANIGSVVRTANAFAVHTVHIVGRRRWNRRGAMVTDRYQRLCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTALPR
ECLLLFGQEGPGITDDARAGAAVTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLGRAW

Sequences:

>Translated_220_residues
MDLAMSALGPGPTEWGAPTGGVGPWAGDLPDDPRYDPVLLRDGDARNVVDAYRYWTREAIIADIDTRRHPLHVAIENFGH
DANIGSVVRTANAFAVHTVHIVGRRRWNRRGAMVTDRYQRLCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTALPR
ECLLLFGQEGPGITDDARAGAAVTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLGRAW
>Mature_220_residues
MDLAMSALGPGPTEWGAPTGGVGPWAGDLPDDPRYDPVLLRDGDARNVVDAYRYWTREAIIADIDTRRHPLHVAIENFGH
DANIGSVVRTANAFAVHTVHIVGRRRWNRRGAMVTDRYQRLCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTALPR
ECLLLFGQEGPGITDDARAGAAVTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLGRAW

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=166, Percent_Identity=28.9156626506024, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 23630; Mature: 23630

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAMSALGPGPTEWGAPTGGVGPWAGDLPDDPRYDPVLLRDGDARNVVDAYRYWTREAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
IADIDTRRHPLHVAIENFGHDANIGSVVRTANAFAVHTVHIVGRRRWNRRGAMVTDRYQR
EEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEEEEHHHHHCCCCCCHHHHHHH
LCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTALPRECLLLFGQEGPGITDDARAG
HHHCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHEEECCCCCCCCCCCCCC
AAVTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLGRAW
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDLAMSALGPGPTEWGAPTGGVGPWAGDLPDDPRYDPVLLRDGDARNVVDAYRYWTREAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
IADIDTRRHPLHVAIENFGHDANIGSVVRTANAFAVHTVHIVGRRRWNRRGAMVTDRYQR
EEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEEEEHHHHHCCCCCCHHHHHHH
LCHHDSTTGLLEFAAGAGLTVVAVDNVPGAARLEQTALPRECLLLFGQEGPGITDDARAG
HHHCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHEEECCCCCCCCCCCCCC
AAVTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLGRAW
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]