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The map label for this gene is yfkO [H]

Identifier: 148653797

GI number: 148653797

Start: 2491081

End: 2491713

Strand: Direct

Name: yfkO [H]

Synonym: PsycPRwf_2000

Alternate gene names: 148653797

Gene position: 2491081-2491713 (Clockwise)

Preceding gene: 148653796

Following gene: 148653798

Centisome position: 83.62

GC content: 45.34

Gene sequence:

>633_bases
ATGTCATTACTAGACCATTTAAACTGGCGTCATGCGGTTAAAGCATTTGACCCAACCCAAAAAGTTAGCCAAGAAAACAT
CGATAAAATCATTGAAGCGGCCAGATTGGCACCGACGTCTTCAGGCCTACAACCCTTTAGACTGTTGGTTGTCGAAAGCC
AAGAGATCAAAGACAAACTGGTTAAAGGTGCACTAAACCCTGAGTGTATGCAAGAGTGCTCACATGTGCTAATTTTCGCT
GGCTGGAATGATTACAGCGATCAAAAGGTCGATGATGTTTATGACATGACCACCGAACAGCGCGGTTTACCACAAGGTCG
TTTTAGCAGTTATACCGATAAGCTAAAAGAGATTTTCAACAACCAAAGCACCCAAGAAAACTTTGAGCACATCGCTCGCC
AAGCTTACATTGCGATGGGTTTTGCTTTGGCACAAGCGGCTGAGCTAAGAGTCGATACCTGCCCTGCTGAAGGCTTTAGC
ACAGATTTAGTAGACGAAGTACTTGAGCTCGATAAGCAAGGTCTCAAAAGTATTGTGCTGATGTATGTCGGCGTGGCTGA
CCCTGAGCGTGACTGGATGGCACCGATGGCCAAAGTCCGTAACGCTGCTGAAGAGTTTGTGATTAAATACTAA

Upstream 100 bases:

>100_bases
ATTGCAAAACAAAGCGTTATCCTCATCTATGAAGTGTGTGAAAGATACACGATTTATTTTATGAATAATTTATAACACCA
TATAAAAAATAAGGGCAACC

Downstream 100 bases:

>100_bases
GCATTTGACAGTCTGTGTGCACCAATAAAGGCCATTCTTAAAGAATGGCCTTTTTATTGAGCGTGTTAACTGATTTTGGG
GCAGAGGTTACCGTCATAAT

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSLLDHLNWRHAVKAFDPTQKVSQENIDKIIEAARLAPTSSGLQPFRLLVVESQEIKDKLVKGALNPECMQECSHVLIFA
GWNDYSDQKVDDVYDMTTEQRGLPQGRFSSYTDKLKEIFNNQSTQENFEHIARQAYIAMGFALAQAAELRVDTCPAEGFS
TDLVDEVLELDKQGLKSIVLMYVGVADPERDWMAPMAKVRNAAEEFVIKY

Sequences:

>Translated_210_residues
MSLLDHLNWRHAVKAFDPTQKVSQENIDKIIEAARLAPTSSGLQPFRLLVVESQEIKDKLVKGALNPECMQECSHVLIFA
GWNDYSDQKVDDVYDMTTEQRGLPQGRFSSYTDKLKEIFNNQSTQENFEHIARQAYIAMGFALAQAAELRVDTCPAEGFS
TDLVDEVLELDKQGLKSIVLMYVGVADPERDWMAPMAKVRNAAEEFVIKY
>Mature_209_residues
SLLDHLNWRHAVKAFDPTQKVSQENIDKIIEAARLAPTSSGLQPFRLLVVESQEIKDKLVKGALNPECMQECSHVLIFAG
WNDYSDQKVDDVYDMTTEQRGLPQGRFSSYTDKLKEIFNNQSTQENFEHIARQAYIAMGFALAQAAELRVDTCPAEGFST
DLVDEVLELDKQGLKSIVLMYVGVADPERDWMAPMAKVRNAAEEFVIKY

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=204, Percent_Identity=28.4313725490196, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23777; Mature: 23646

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLDHLNWRHAVKAFDPTQKVSQENIDKIIEAARLAPTSSGLQPFRLLVVESQEIKDKL
CCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECHHHHHHHH
VKGALNPECMQECSHVLIFAGWNDYSDQKVDDVYDMTTEQRGLPQGRFSSYTDKLKEIFN
HHCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NQSTQENFEHIARQAYIAMGFALAQAAELRVDTCPAEGFSTDLVDEVLELDKQGLKSIVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
MYVGVADPERDWMAPMAKVRNAAEEFVIKY
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLLDHLNWRHAVKAFDPTQKVSQENIDKIIEAARLAPTSSGLQPFRLLVVESQEIKDKL
CHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEECHHHHHHHH
VKGALNPECMQECSHVLIFAGWNDYSDQKVDDVYDMTTEQRGLPQGRFSSYTDKLKEIFN
HHCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NQSTQENFEHIARQAYIAMGFALAQAAELRVDTCPAEGFSTDLVDEVLELDKQGLKSIVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
MYVGVADPERDWMAPMAKVRNAAEEFVIKY
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]