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The map label for this gene is wax-dgaT [H]

Identifier: 148653638

GI number: 148653638

Start: 2289930

End: 2291390

Strand: Direct

Name: wax-dgaT [H]

Synonym: PsycPRwf_1841

Alternate gene names: 148653638

Gene position: 2289930-2291390 (Clockwise)

Preceding gene: 148653637

Following gene: 148653642

Centisome position: 76.87

GC content: 46.34

Gene sequence:

>1461_bases
ATGCGAATTTTATCTTTGGTAGAGCAGCTATTTTTTTTATTAGAAAAGCACAAACAGCCCATGCACGTCGGTGGGCTGTT
TTTGTTTGATATACCAGACAATAGTGACAATAATTTTGTCGCAGATTTGGTTCGTCAAATGCGCAGCTCTACCACACCGC
CGACTTTTCCATTTGATCAAGTGGTGCACAATCAAACCTTTTGGAAAAGTGCAGACAAGGTAGATATTCACTATCACTTT
CACCACATGGTACTGCCAAAGCCTTATTCAAAACAGGCCCTATATAGTTATGTGTCGGATGTGCATGCCAATATGCTAGA
TAAGGGCTACCCGCTGTGGGAATGTCATATTATTGAGGGTATTGAAGCTGATCATGTCGATGTCAGCGCCCCCTCTAAGC
CCTTACACCCAAACCAGTTACCTATAAATCAGGCTGAGCCAAAGCCCAAGTCCTCTCAAAAGCCTGCGCCAAAAAAGCAA
TTTGCTTTGTATTTTAAGATTCACCATGCCTTGGTGGATGGGGTGGCAGCCATGCGTTTGGTTGAGAGGTCTTTATCTGC
CTCACAGCATGAGGTGATGAGCTTGCCACCTTGGGCCTTAATGCGCCGAGATAAAAAGCATCTGGCCGCCCTTACCCCAC
AAAAACGCTCGGTTAGCGGTATTGTTAAAGAGCAGGTGGGCAGCATCAAACCGGTATTTACTGAACTCAAAAATGGCCTC
AGGCAGCGCACAGATCCTGGCCATGTCTCAACCTTACAAGCACCCAGCAGCTTGCTTAATCAGCGCATATCCAATTCACG
CATATTCATGGCGCATACCTTCCATTTGAGCCGGTTTAAAAGCCTAGCCCAGCAGCTCGATGTGAGTATTAATGATGTGG
CCTTGGCGGTATGCTCTGGGGCACTGCGCAGCTATTTAATCAGTGAAAACGCGCTGCCCAAAGAGCCACTCGTTGCCTTT
GTGCCCGTATCGCTTCGAAAAGATCAAAGCGCCTCGGGCAATCAAACATCACTGGTATTGTGTGACTTGGGGACCGATCA
GGCCAGCCCCAAGCAGCGATTACAAGCCATTCATGACAGTATGACCATCGGTAAAGATAAGTTTGGGCGCTTAGATCAGG
CGCAAGTCATCAACTACAGTGCCATAACTTATGCCTGGGAGGGGGTTAATTTATTAACCGGCGCTTACCCCAAAAAGCAG
GCTTTTAATATTCTCATATCCAATGTTCCTGGTCCCAAACAGCAACTGTATTGGGATGGTGCTGCGCTGAGTGCACTGTA
TCCGGCTTCTGTTGTTTTTGATGGTCAAGCGCTAAATATAACCTTTACCAGTTATCTCAATCAGGTGCATTTTGGAATTA
TTGGCTGTGGTAAAAATCTGCCCAATATTCAAAATCTGGTGCCTTTATTGGAGCAAGCGCTATTGCTACTTGAGGCAGAC
AGTAAAGAGCGATCTACCTAA

Upstream 100 bases:

>100_bases
GTAGCTTACTGTTAGATGATGACTAAGAACATCAAGACAAATGGCGATAAGGCAGGGCTGGCTTATCGGGTTTTACTAAA
GACTGTTAATAAGGTTTTTT

Downstream 100 bases:

>100_bases
GATTAAAAACCCAGCTTTAAGCCAAGTTTTTGTAGCAGCCATAAGCCATAAGTGACTGCGATATAATTAATAAATAAGCC
AATGGCGCCACTAATCACCA

Product: acyltransferase ws/dgat/mgat family protein

Products: NA

Alternate protein names: Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRILSLVEQLFFLLEKHKQPMHVGGLFLFDIPDNSDNNFVADLVRQMRSSTTPPTFPFDQVVHNQTFWKSADKVDIHYHF
HHMVLPKPYSKQALYSYVSDVHANMLDKGYPLWECHIIEGIEADHVDVSAPSKPLHPNQLPINQAEPKPKSSQKPAPKKQ
FALYFKIHHALVDGVAAMRLVERSLSASQHEVMSLPPWALMRRDKKHLAALTPQKRSVSGIVKEQVGSIKPVFTELKNGL
RQRTDPGHVSTLQAPSSLLNQRISNSRIFMAHTFHLSRFKSLAQQLDVSINDVALAVCSGALRSYLISENALPKEPLVAF
VPVSLRKDQSASGNQTSLVLCDLGTDQASPKQRLQAIHDSMTIGKDKFGRLDQAQVINYSAITYAWEGVNLLTGAYPKKQ
AFNILISNVPGPKQQLYWDGAALSALYPASVVFDGQALNITFTSYLNQVHFGIIGCGKNLPNIQNLVPLLEQALLLLEAD
SKERST

Sequences:

>Translated_486_residues
MRILSLVEQLFFLLEKHKQPMHVGGLFLFDIPDNSDNNFVADLVRQMRSSTTPPTFPFDQVVHNQTFWKSADKVDIHYHF
HHMVLPKPYSKQALYSYVSDVHANMLDKGYPLWECHIIEGIEADHVDVSAPSKPLHPNQLPINQAEPKPKSSQKPAPKKQ
FALYFKIHHALVDGVAAMRLVERSLSASQHEVMSLPPWALMRRDKKHLAALTPQKRSVSGIVKEQVGSIKPVFTELKNGL
RQRTDPGHVSTLQAPSSLLNQRISNSRIFMAHTFHLSRFKSLAQQLDVSINDVALAVCSGALRSYLISENALPKEPLVAF
VPVSLRKDQSASGNQTSLVLCDLGTDQASPKQRLQAIHDSMTIGKDKFGRLDQAQVINYSAITYAWEGVNLLTGAYPKKQ
AFNILISNVPGPKQQLYWDGAALSALYPASVVFDGQALNITFTSYLNQVHFGIIGCGKNLPNIQNLVPLLEQALLLLEAD
SKERST
>Mature_486_residues
MRILSLVEQLFFLLEKHKQPMHVGGLFLFDIPDNSDNNFVADLVRQMRSSTTPPTFPFDQVVHNQTFWKSADKVDIHYHF
HHMVLPKPYSKQALYSYVSDVHANMLDKGYPLWECHIIEGIEADHVDVSAPSKPLHPNQLPINQAEPKPKSSQKPAPKKQ
FALYFKIHHALVDGVAAMRLVERSLSASQHEVMSLPPWALMRRDKKHLAALTPQKRSVSGIVKEQVGSIKPVFTELKNGL
RQRTDPGHVSTLQAPSSLLNQRISNSRIFMAHTFHLSRFKSLAQQLDVSINDVALAVCSGALRSYLISENALPKEPLVAF
VPVSLRKDQSASGNQTSLVLCDLGTDQASPKQRLQAIHDSMTIGKDKFGRLDQAQVINYSAITYAWEGVNLLTGAYPKKQ
AFNILISNVPGPKQQLYWDGAALSALYPASVVFDGQALNITFTSYLNQVHFGIIGCGKNLPNIQNLVPLLEQALLLLEAD
SKERST

Specific function: Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS us

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20; =2.3.1.75 [H]

Molecular weight: Translated: 54201; Mature: 54201

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILSLVEQLFFLLEKHKQPMHVGGLFLFDIPDNSDNNFVADLVRQMRSSTTPPTFPFDQ
CHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
VVHNQTFWKSADKVDIHYHFHHMVLPKPYSKQALYSYVSDVHANMLDKGYPLWECHIIEG
HHCCCHHHCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
IEADHVDVSAPSKPLHPNQLPINQAEPKPKSSQKPAPKKQFALYFKIHHALVDGVAAMRL
CCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHH
VERSLSASQHEVMSLPPWALMRRDKKHLAALTPQKRSVSGIVKEQVGSIKPVFTELKNGL
HHHHHCCCHHHHHCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
RQRTDPGHVSTLQAPSSLLNQRISNSRIFMAHTFHLSRFKSLAQQLDVSINDVALAVCSG
HHCCCCCCCEEHHCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ALRSYLISENALPKEPLVAFVPVSLRKDQSASGNQTSLVLCDLGTDQASPKQRLQAIHDS
HHHHHHHCCCCCCCCCCEEEEECEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
MTIGKDKFGRLDQAQVINYSAITYAWEGVNLLTGAYPKKQAFNILISNVPGPKQQLYWDG
HHCCCHHCCCCCHHHEEEEEHEEEEECCCHHHCCCCCCHHHHHHEEECCCCCHHHEEECC
AALSALYPASVVFDGQALNITFTSYLNQVHFGIIGCGKNLPNIQNLVPLLEQALLLLEAD
HHHHHHCCCEEEECCCEEEEEHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCC
SKERST
CCCCCC
>Mature Secondary Structure
MRILSLVEQLFFLLEKHKQPMHVGGLFLFDIPDNSDNNFVADLVRQMRSSTTPPTFPFDQ
CHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
VVHNQTFWKSADKVDIHYHFHHMVLPKPYSKQALYSYVSDVHANMLDKGYPLWECHIIEG
HHCCCHHHCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
IEADHVDVSAPSKPLHPNQLPINQAEPKPKSSQKPAPKKQFALYFKIHHALVDGVAAMRL
CCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHH
VERSLSASQHEVMSLPPWALMRRDKKHLAALTPQKRSVSGIVKEQVGSIKPVFTELKNGL
HHHHHCCCHHHHHCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
RQRTDPGHVSTLQAPSSLLNQRISNSRIFMAHTFHLSRFKSLAQQLDVSINDVALAVCSG
HHCCCCCCCEEHHCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ALRSYLISENALPKEPLVAFVPVSLRKDQSASGNQTSLVLCDLGTDQASPKQRLQAIHDS
HHHHHHHCCCCCCCCCCEEEEECEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
MTIGKDKFGRLDQAQVINYSAITYAWEGVNLLTGAYPKKQAFNILISNVPGPKQQLYWDG
HHCCCHHCCCCCHHHEEEEEHEEEEECCCHHHCCCCCCHHHHHHEEECCCCCHHHEEECC
AALSALYPASVVFDGQALNITFTSYLNQVHFGIIGCGKNLPNIQNLVPLLEQALLLLEAD
HHHHHHCCCEEEECCCEEEEEHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCC
SKERST
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12502715 [H]