| Definition | Psychrobacter sp. PRwf-1 chromosome, complete genome. |
|---|---|
| Accession | NC_009524 |
| Length | 2,978,976 |
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The map label for this gene is dnaG [H]
Identifier: 148653372
GI number: 148653372
Start: 1932555
End: 1934672
Strand: Direct
Name: dnaG [H]
Synonym: PsycPRwf_1574
Alternate gene names: 148653372
Gene position: 1932555-1934672 (Clockwise)
Preceding gene: 148653371
Following gene: 148653373
Centisome position: 64.87
GC content: 48.11
Gene sequence:
>2118_bases ATGAGCATCCCTAACCACATTCTTGATCAGCTCAATAGCCAAGCCGACTTGGTAGGGATTATTGGCAAACACACCACGCT TAAGCGCGCGGGAAAAGAGTTTAAGGGCAATTGTCCTTTTCATGGCGAAAAAACGCCCTCTTTTTATGTTAATCCCCAAA AAAATGTCTATAACTGCTTTGGCTGCGGTGTGAGTGGTAATGCCATTAAGTTTTTGCGGGAATACGAAAACCAAACCTTT ATGGAGGCAGTACAAGAGCTGTCACGCCAGACGGGTATAGAGATTCCCAAAGAAGACAACAAAGACTTGCGTTATAAACG CAGCGCCAAACCCACGCCCACGGCCCCTGCGCCCTCGTGGACTGGCAATGGCAAACGCTTAAATGACACGCACACTGCGG CGACGACACCGCATTCGGGCCAAACTAATGGCTTAGCCACTAATACGACATCAGTGGCTGATGCGCCCCCTGCTTATTTC GATGAAGACAGCTATTTTAATTTAGATTCAGCGCCGCCCAGTGATTGGGATATGGGCGACACACCTGGTGATAACCTTGG TTATGACAGCGGCTTTACAGATATGTCTTCCTCTAACTTTGGTGGTCAAGCCACCATAGAGCAAGACGGCAACTTATATG AGCTACTGATCCAAATTGGCCAATTCTATCAAAACAATCTGCGTAATAACCTGCACGCCATGGCGTATTTTACAGAGCGG GGATTAACCGATGCCACCATTAATGAATTTGGTCTGGGTTATGCACCCACCGGCTGGCAGCATTTAGAAGAGGCCTTTCC GCAGGATATTGCAGGTCTAAAAGCTTTGGGTCTGGTGCGTCAATCTGAAAAGGGTCGCGACTATGATCTGCTGCGTGATC GGGTTATTTTTCCTATTCGCGACAATCAAGGCCGGATTATTGGCTTTGCCGGTCGGGCATTAGACAACGAAGTTAAGCCC AAATACATCAACTCCAGTGACTCCCCTGTCTTTCATAAGCAGCATGTACTCTACGGCTATTACGAGTCACGTCAACAGCG AGCGAACGATTGGCTGGTGGTTGAAGGCTATATGGATGTCATTGCCTTATATCAAGCGGGTATCTATGGCGCAGTGGCCT CAATGGGTACCTCGATTAACGAAACCCAAATCGCCCGCTTATTGCAAATGAACCCCACCTTAACCTTATGTTTTGATGGT GACAGTGCCGGTCAAAAGGCCGCTTGGCGTACGCTTGAGGTGGCCTTACCTGTGCTAAGCGATGACAAAGAGCTGCGCTT TTTAACCCTACCAGGTGGGCATGACCCAGACACTTACATTGCCATGCAGGGTGTGGAGGCGATGCGAGAGCATATTAAGC GCGCCATGCCGTTATCGCAATACATTTTTGCTTATCTCAGCGAGCGCTATGACTTAAGCTTAGCCGAAGGTAAAGCCAAG CTGATGTCACAAGTACGTCAGTTGACCAATAATCTGCCCAAGGGTTCAAGCTTTAAGTATCTGCTTAATAACGATATTTA TCAAAAGCTTGGCGGGCGCAAAAATCAAAAAAACTCCGCCCATGATGTCTTATTAGACTTCGACAGCGACATGACGGTGA GCCGACAGCTCGAGTTGTGTTTACTGTTTGCCCCACACTTATTGGCAGAAGACCCCATCAAGCGACTGTGGGAGGTATCT GGTGTGGCAGATATTAAGATACCTGAAAAATCGATAGGCACACAGCAAGCACCCGTAGCGACCCCACCTTTGCCAAGCTG GGAGGATTTTAATAGCCCAAGTCTTACCCAGCTGACTCAGACTATCATTGGTCTTACCCCTTATTTGCCTGAGGACACCA ATGCGGCCGCTCACTTTATTTTGGCCAATTTGCCAAATGGCCTACAACAAAATCTTGCCACCCGCTGGCATCCATTTTGG TCCAATCTCAACCAGCGCGGGATTATTGACGTAGATGATTTGGTAGATGACTTGTTAACTCAGCTGATGCTACAAGCCTT ATCAAAACAGATGAGTCAGTCACCAGGGCTGGTGATTACCAGCCATCTGAACCGGCAGCGCCAGACCTTAATGAACTGGC TTAAAAAGCAGCAAGCTGAGCAGTCCTCCAAGATATAA
Upstream 100 bases:
>100_bases TTATTGCGCTTTAATGCTCTATCATGTCACTTGTCTCTTGTGAATTTAAGTGATTGACCTCATCTTCCTCACAACCCACT TTGTCAGATAAAGCGCCGTT
Downstream 100 bases:
>100_bases TCCTGAGGGTCTAACAATCTTGAGGGTCTAACAGCGCCCCTACACTGATAACTGGTTTTAAATTGTGCTTTAATCTGGCA TTTATCAACCTTTTTAGACC
Product: DNA primase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 705; Mature: 704
Protein sequence:
>705_residues MSIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCFGCGVSGNAIKFLREYENQTF MEAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSWTGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYF DEDSYFNLDSAPPSDWDMGDTPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTER GLTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIRDNQGRIIGFAGRALDNEVKP KYINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDVIALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDG DSAGQKAAWRTLEVALPVLSDDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAK LMSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELCLLFAPHLLAEDPIKRLWEVS GVADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQTIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFW SNLNQRGIIDVDDLVDDLLTQLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI
Sequences:
>Translated_705_residues MSIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCFGCGVSGNAIKFLREYENQTF MEAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSWTGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYF DEDSYFNLDSAPPSDWDMGDTPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTER GLTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIRDNQGRIIGFAGRALDNEVKP KYINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDVIALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDG DSAGQKAAWRTLEVALPVLSDDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAK LMSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELCLLFAPHLLAEDPIKRLWEVS GVADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQTIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFW SNLNQRGIIDVDDLVDDLLTQLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI >Mature_704_residues SIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCFGCGVSGNAIKFLREYENQTFM EAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSWTGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYFD EDSYFNLDSAPPSDWDMGDTPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTERG LTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIRDNQGRIIGFAGRALDNEVKPK YINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDVIALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDGD SAGQKAAWRTLEVALPVLSDDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAKL MSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELCLLFAPHLLAEDPIKRLWEVSG VADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQTIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFWS NLNQRGIIDVDDLVDDLLTQLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI
Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]
COG id: COG0358
COG function: function code L; DNA primase (bacterial type)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Escherichia coli, GI1789447, Length=319, Percent_Identity=42.6332288401254, Blast_Score=254, Evalue=2e-68,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016136 - InterPro: IPR013264 - InterPro: IPR019475 - InterPro: IPR006295 - InterPro: IPR013173 - InterPro: IPR006171 - InterPro: IPR002694 [H]
Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]
EC number: 2.7.7.-
Molecular weight: Translated: 78710; Mature: 78579
Theoretical pI: Translated: 5.74; Mature: 5.74
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCF CCCCHHHHHHCCCCCCEEEECCCCHHHHHHCHHCCCCCCCCCCCCCCEEECCCCCCEEEE GCGVSGNAIKFLREYENQTFMEAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSW ECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCC TGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYFDEDSYFNLDSAPPSDWDMGD CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC TPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTER CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHEEHHHC GLTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIR CCCCCCHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEE DNQGRIIGFAGRALDNEVKPKYINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDV CCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHEEHHHHHHHHCCCCCEEEHHHHHH IALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDGDSAGQKAAWRTLEVALPVLS HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHEEEECCCC DDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAK CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH LMSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELC HHHHHHHHHHCCCCCCCEEEHHCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHH LLFAPHLLAEDPIKRLWEVSGVADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQ HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH TIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFWSNLNQRGIIDVDDLVDDLLT HHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCCEEHHHHHHHHHH QLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure SIPNHILDQLNSQADLVGIIGKHTTLKRAGKEFKGNCPFHGEKTPSFYVNPQKNVYNCF CCCHHHHHHCCCCCCEEEECCCCHHHHHHCHHCCCCCCCCCCCCCCEEECCCCCCEEEE GCGVSGNAIKFLREYENQTFMEAVQELSRQTGIEIPKEDNKDLRYKRSAKPTPTAPAPSW ECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCC TGNGKRLNDTHTAATTPHSGQTNGLATNTTSVADAPPAYFDEDSYFNLDSAPPSDWDMGD CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC TPGDNLGYDSGFTDMSSSNFGGQATIEQDGNLYELLIQIGQFYQNNLRNNLHAMAYFTER CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHEEHHHC GLTDATINEFGLGYAPTGWQHLEEAFPQDIAGLKALGLVRQSEKGRDYDLLRDRVIFPIR CCCCCCHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEE DNQGRIIGFAGRALDNEVKPKYINSSDSPVFHKQHVLYGYYESRQQRANDWLVVEGYMDV CCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHEEHHHHHHHHCCCCCEEEHHHHHH IALYQAGIYGAVASMGTSINETQIARLLQMNPTLTLCFDGDSAGQKAAWRTLEVALPVLS HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHEEEECCCC DDKELRFLTLPGGHDPDTYIAMQGVEAMREHIKRAMPLSQYIFAYLSERYDLSLAEGKAK CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH LMSQVRQLTNNLPKGSSFKYLLNNDIYQKLGGRKNQKNSAHDVLLDFDSDMTVSRQLELC HHHHHHHHHHCCCCCCCEEEHHCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHH LLFAPHLLAEDPIKRLWEVSGVADIKIPEKSIGTQQAPVATPPLPSWEDFNSPSLTQLTQ HHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH TIIGLTPYLPEDTNAAAHFILANLPNGLQQNLATRWHPFWSNLNQRGIIDVDDLVDDLLT HHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCCEEHHHHHHHHHH QLMLQALSKQMSQSPGLVITSHLNRQRQTLMNWLKKQQAEQSSKI HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]