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The map label for this gene is rpoD [H]

Identifier: 148653373

GI number: 148653373

Start: 1935099

End: 1936970

Strand: Direct

Name: rpoD [H]

Synonym: PsycPRwf_1575

Alternate gene names: 148653373

Gene position: 1935099-1936970 (Clockwise)

Preceding gene: 148653372

Following gene: 148653374

Centisome position: 64.96

GC content: 46.74

Gene sequence:

>1872_bases
ATGACCGATAAGGCAGCCTCTCAATCTACCTCTCAACTAGCCACACTTATCCAGATGGGTAAAGAGCAAGGCTATTTGAC
TTATGCTGAGGTTAACGACCAACTTCCCGCCTCAATCACCGAAAGCGACCAAATTGAAGACATCGTTCAAATGCTTACGG
ATGTTGGTATTAAAATCTTTGAATCAGCGCCAGATGCTGATGATATCTTGATGAACGATGGTGCTAACGATGACGACATG
GCCACAGATGAAGCTGCTGCTGTATTGGCTGCGGTAGAAACTGAGCCTGGTCGCACCACCGACCCTGTGCGTATGTATAT
GCGTGAAATGGGTACGGTTGACTTATTGACGCGTGAAGGTGAAATCGTTATCGCCAAGCGTATCGAAGAGGGTATCCGTG
ATGTCCAGCATGCCATGGCTTACTGGCCAGGTACTGTGCAGTTTATCCTAGATGAATATGACTTAGTAGAAGCGGGCGAG
AAAAAACTCTCTGACGTCATCACTGGTTTTTTAGATCCTGAAGAGATTGAACCGGCAGTTGTGGAAGAAGACGAAGAAAA
AATCCCCGTTATCACCACCAAATCTAAAGCTAAAGATGTCGATGACGAAGATGATGAAGAAGAAGCTGAGGAAGAGGAAG
AAGCTGAAGAAAGCGGTGGCCTAGATCCTGAGCTGGTTCGTCAACGTGTCGAGGAGATCCGTGAATTATTTGCGGTGGCA
AAACAAGCCCTAGAGCAGTATGGTCGTGGCAGTGCGCAAGCTGAAGAAGCCTATGCTCAGCTGGCCGAACGCTTTATGCT
ATTAAAGCTTAACAACCGCATGGCGGATAATGTTATGGCTATTATGCATGATGTTTATGACGATGTGCGTAAGCAAGAGC
GTCAAATCATGCGCTTGGTCATTCGCCGCGGTAAAATGCCTCGTAGCGAGTTCCGTAAGACTTTCCCAAGTAACGAGACC
AACGTAGATTGGCTAACTGATCGCCTAAAAGGCAACCCTAAATTTGCAGATAGCTTAGCCAAAGTGGTCGACGATGTGGT
GGTTTTACAGCGCCGTATCCGCGACCATGAAGACAAGCTGGACATGCAAATTACCGAAATGAAAGAAGTGGCGCGCCGTA
TGGCCATCGGTGAGGCAAAAGCCCGCCGTGCCAAAAAAGAGATGGTCGAAGCCAACTTACGTTTGGTAATCTCTATTGCT
AAGAAATATACCAACCGTGGCCTACAGTTCTTAGACTTGATTCAAGAAGGTAATATTGGCTTGATGAAAGCGGTAGATAA
ATTTGAATATCGCCGTGGTTATAAGTTCTCAACTTATGCCACTTGGTGGATTCGTCAGGCCATTACCCGCTCTATTGCTG
ACCAAGCCCGCACCATTCGTATTCCAGTGCATATGATTGAGACCATCAACAAAATCAACCGTGTGTCTCGTCAATTGTTG
CAAGAGATGGGTCGTGAGCCCACACCTGAGGAATTAGGCGAACGTTTAGAAATGGATGAGGTTAAGGTTCGTAAAGTATT
AAAAATTGCCAAAGAGCCTATCTCTATGGAGACCCCCATCGGTGATGATGAAGATTCACACCTAGGTGATTTCATTGAGG
ACCAGACCATCTCAAGCCCTGTAGAAGATGCCACCTCAGCAGGTCTACAAGAAGCGACTCGAGATGTGCTGAGCAACTTA
ACAGAACGTGAAGCCAAGGTGCTACGCATGCGTTTTGGTATTGATATGCCAACCGATCACACCCTAGAAGAGGTTGGTAA
GCAGTTCGATGTGACGCGTGAGCGTATTCGTCAGATTGAAGCCAAAGCGTTACGTAAACTGCGTCACCCTTCTCGCTCTG
AGCATTTACGTTCGTTCTTAGAAAACGACTAA

Upstream 100 bases:

>100_bases
GCAAAAACTAAAACAATAAGACAACCATCTGGCCATCTAAAAACCATGTGCACCCCTCACTCTACCTTTAACCCACATCT
TCTATTGCAAGCGAGATATT

Downstream 100 bases:

>100_bases
GCCAAGGTTGAGTCAACGCCCATAACCTGTTGTAGCAAAAAAGCTGAGAATCTCTCAGCTTTTTTGTGTTTAAAGCCTAT
GAATACCTCTGTCTAAAGCA

Product: RNA polymerase, sigma 70 subunit, RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MTDKAASQSTSQLATLIQMGKEQGYLTYAEVNDQLPASITESDQIEDIVQMLTDVGIKIFESAPDADDILMNDGANDDDM
ATDEAAAVLAAVETEPGRTTDPVRMYMREMGTVDLLTREGEIVIAKRIEEGIRDVQHAMAYWPGTVQFILDEYDLVEAGE
KKLSDVITGFLDPEEIEPAVVEEDEEKIPVITTKSKAKDVDDEDDEEEAEEEEEAEESGGLDPELVRQRVEEIRELFAVA
KQALEQYGRGSAQAEEAYAQLAERFMLLKLNNRMADNVMAIMHDVYDDVRKQERQIMRLVIRRGKMPRSEFRKTFPSNET
NVDWLTDRLKGNPKFADSLAKVVDDVVVLQRRIRDHEDKLDMQITEMKEVARRMAIGEAKARRAKKEMVEANLRLVISIA
KKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRVSRQLL
QEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQTISSPVEDATSAGLQEATRDVLSNL
TEREAKVLRMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND

Sequences:

>Translated_623_residues
MTDKAASQSTSQLATLIQMGKEQGYLTYAEVNDQLPASITESDQIEDIVQMLTDVGIKIFESAPDADDILMNDGANDDDM
ATDEAAAVLAAVETEPGRTTDPVRMYMREMGTVDLLTREGEIVIAKRIEEGIRDVQHAMAYWPGTVQFILDEYDLVEAGE
KKLSDVITGFLDPEEIEPAVVEEDEEKIPVITTKSKAKDVDDEDDEEEAEEEEEAEESGGLDPELVRQRVEEIRELFAVA
KQALEQYGRGSAQAEEAYAQLAERFMLLKLNNRMADNVMAIMHDVYDDVRKQERQIMRLVIRRGKMPRSEFRKTFPSNET
NVDWLTDRLKGNPKFADSLAKVVDDVVVLQRRIRDHEDKLDMQITEMKEVARRMAIGEAKARRAKKEMVEANLRLVISIA
KKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRVSRQLL
QEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQTISSPVEDATSAGLQEATRDVLSNL
TEREAKVLRMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND
>Mature_622_residues
TDKAASQSTSQLATLIQMGKEQGYLTYAEVNDQLPASITESDQIEDIVQMLTDVGIKIFESAPDADDILMNDGANDDDMA
TDEAAAVLAAVETEPGRTTDPVRMYMREMGTVDLLTREGEIVIAKRIEEGIRDVQHAMAYWPGTVQFILDEYDLVEAGEK
KLSDVITGFLDPEEIEPAVVEEDEEKIPVITTKSKAKDVDDEDDEEEAEEEEEAEESGGLDPELVRQRVEEIRELFAVAK
QALEQYGRGSAQAEEAYAQLAERFMLLKLNNRMADNVMAIMHDVYDDVRKQERQIMRLVIRRGKMPRSEFRKTFPSNETN
VDWLTDRLKGNPKFADSLAKVVDDVVVLQRRIRDHEDKLDMQITEMKEVARRMAIGEAKARRAKKEMVEANLRLVISIAK
KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRVSRQLLQ
EMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQTISSPVEDATSAGLQEATRDVLSNLT
EREAKVLRMRFGIDMPTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHLRSFLEND

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=615, Percent_Identity=61.4634146341463, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=246, Percent_Identity=41.0569105691057, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1789871, Length=254, Percent_Identity=28.3464566929134, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.2178217821782, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 71184; Mature: 71053

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKAASQSTSQLATLIQMGKEQGYLTYAEVNDQLPASITESDQIEDIVQMLTDVGIKIF
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHH
ESAPDADDILMNDGANDDDMATDEAAAVLAAVETEPGRTTDPVRMYMREMGTVDLLTREG
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHEEECCC
EIVIAKRIEEGIRDVQHAMAYWPGTVQFILDEYDLVEAGEKKLSDVITGFLDPEEIEPAV
CEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCH
VEEDEEKIPVITTKSKAKDVDDEDDEEEAEEEEEAEESGGLDPELVRQRVEEIRELFAVA
HCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KQALEQYGRGSAQAEEAYAQLAERFMLLKLNNRMADNVMAIMHDVYDDVRKQERQIMRLV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRRGKMPRSEFRKTFPSNETNVDWLTDRLKGNPKFADSLAKVVDDVVVLQRRIRDHEDKL
HHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMQITEMKEVARRMAIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
LMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRVSRQLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
QEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQTISSP
HHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
VEDATSAGLQEATRDVLSNLTEREAKVLRMRFGIDMPTDHTLEEVGKQFDVTRERIRQIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKALRKLRHPSRSEHLRSFLEND
HHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TDKAASQSTSQLATLIQMGKEQGYLTYAEVNDQLPASITESDQIEDIVQMLTDVGIKIF
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHH
ESAPDADDILMNDGANDDDMATDEAAAVLAAVETEPGRTTDPVRMYMREMGTVDLLTREG
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHEEECCC
EIVIAKRIEEGIRDVQHAMAYWPGTVQFILDEYDLVEAGEKKLSDVITGFLDPEEIEPAV
CEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCH
VEEDEEKIPVITTKSKAKDVDDEDDEEEAEEEEEAEESGGLDPELVRQRVEEIRELFAVA
HCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KQALEQYGRGSAQAEEAYAQLAERFMLLKLNNRMADNVMAIMHDVYDDVRKQERQIMRLV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRRGKMPRSEFRKTFPSNETNVDWLTDRLKGNPKFADSLAKVVDDVVVLQRRIRDHEDKL
HHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMQITEMKEVARRMAIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCH
LMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKINRVSRQLL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
QEMGREPTPEELGERLEMDEVKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDQTISSP
HHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
VEDATSAGLQEATRDVLSNLTEREAKVLRMRFGIDMPTDHTLEEVGKQFDVTRERIRQIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKALRKLRHPSRSEHLRSFLEND
HHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]