Click here to switch to the map view.

The map label for this gene is benM [H]

Identifier: 148653002

GI number: 148653002

Start: 1461964

End: 1462926

Strand: Direct

Name: benM [H]

Synonym: PsycPRwf_1196

Alternate gene names: 148653002

Gene position: 1461964-1462926 (Clockwise)

Preceding gene: 148653001

Following gene: 148653003

Centisome position: 49.08

GC content: 47.66

Gene sequence:

>963_bases
ATGCAGTTAAAAAGATTGACCGCCTTTCATAGCGTAGCACGCTTAAAAAGCTTTAGTGAGGCAGTATCACAAACCCAGCT
GTCACAGCCGACTTTAAGCCGCTTAATCAAGCAGTTAGAGGATGATCTGGGCGTTATCCTCATTGACCGCTATCATCGTC
CGTTACAGCTGACTGAGGCGGGCAGATTTTTTTATGAAAAAATTGATATCATCTTAAAAGAGCTCGATAGCACCATCAGT
CTGACCCAAAAAATCGCGCATCCGCCAGATACCCTAAACATTGGCTTTGTACCCTCAGTGCTTTATGGGCTACTGCCGAC
CATTATCTCACGTCTTAAGCAGCGCTTGCCGAATTTGGAGGTGCATTTAAAAGACATCAGTTCCTTCAAACAGATCGGTG
CGCTCAAGGCCGGCGAGATTGACGTGGGTTTTGGCCGATTTGCCCATAACGATGAGCATATTTATCAGCTGTTACTGCGT
CATGAACGCTACGTCGCCGCCCTTCCCAATACTCATGAACTGGCTGGCAGCCATGATATTCACTTATCACAGCTGCAAAA
TAGCCGAGTTATCTTATACCACCAAACCCATCTTCCTGTCCCTCAACTTGCCAGCGCTTCAACAACTGAGGCTCAAAACA
CAGACCCTAGTCTTATTAGCGAGCCGCTGCTGCATCTGTTTGCCAAGCGCTCAGCCTTACCGCATCACACCACCTCGGTT
AGTGACATTCAGTTGGCATTAGGCTTGGTAGCCGCCGGTGAAGGCATAACGTTAGTGCCTGATAGCCTAAAATCAGTACG
CTCTGAGCAAATCAGTTACCGTACCTTAATTCATGAAGATGCCACCTCACCGATTTACATCTGTAGCCTGACCACCCCCA
CGCACCCAGCCTTTACTACCTTACTTGAGGTGATTTATGCCATTTATGAAGAGCGTGGTATCACCTATCGCCGAGTGATC
TAA

Upstream 100 bases:

>100_bases
TAACCAATGGCGCTATGGTATTAGAAGATGGTCGCGAGATTGTGTTTAAGTAAGCACTATTTGGTGCAATGTATGTGTTT
TAACGTATGGTGAGTGAGTG

Downstream 100 bases:

>100_bases
TTCTTTATCAGCTGTATTTTCTACACAAATTGTAATTTTTTGTAAAAAATCATCTGGTTTTTAATTGCTCTATTTTGCAT
ATTTTAAATACGGCTTAAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MQLKRLTAFHSVARLKSFSEAVSQTQLSQPTLSRLIKQLEDDLGVILIDRYHRPLQLTEAGRFFYEKIDIILKELDSTIS
LTQKIAHPPDTLNIGFVPSVLYGLLPTIISRLKQRLPNLEVHLKDISSFKQIGALKAGEIDVGFGRFAHNDEHIYQLLLR
HERYVAALPNTHELAGSHDIHLSQLQNSRVILYHQTHLPVPQLASASTTEAQNTDPSLISEPLLHLFAKRSALPHHTTSV
SDIQLALGLVAAGEGITLVPDSLKSVRSEQISYRTLIHEDATSPIYICSLTTPTHPAFTTLLEVIYAIYEERGITYRRVI

Sequences:

>Translated_320_residues
MQLKRLTAFHSVARLKSFSEAVSQTQLSQPTLSRLIKQLEDDLGVILIDRYHRPLQLTEAGRFFYEKIDIILKELDSTIS
LTQKIAHPPDTLNIGFVPSVLYGLLPTIISRLKQRLPNLEVHLKDISSFKQIGALKAGEIDVGFGRFAHNDEHIYQLLLR
HERYVAALPNTHELAGSHDIHLSQLQNSRVILYHQTHLPVPQLASASTTEAQNTDPSLISEPLLHLFAKRSALPHHTTSV
SDIQLALGLVAAGEGITLVPDSLKSVRSEQISYRTLIHEDATSPIYICSLTTPTHPAFTTLLEVIYAIYEERGITYRRVI
>Mature_320_residues
MQLKRLTAFHSVARLKSFSEAVSQTQLSQPTLSRLIKQLEDDLGVILIDRYHRPLQLTEAGRFFYEKIDIILKELDSTIS
LTQKIAHPPDTLNIGFVPSVLYGLLPTIISRLKQRLPNLEVHLKDISSFKQIGALKAGEIDVGFGRFAHNDEHIYQLLLR
HERYVAALPNTHELAGSHDIHLSQLQNSRVILYHQTHLPVPQLASASTTEAQNTDPSLISEPLLHLFAKRSALPHHTTSV
SDIQLALGLVAAGEGITLVPDSLKSVRSEQISYRTLIHEDATSPIYICSLTTPTHPAFTTLLEVIYAIYEERGITYRRVI

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=315, Percent_Identity=27.6190476190476, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=24.9158249158249, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790399, Length=261, Percent_Identity=25.6704980842912, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI2367136, Length=302, Percent_Identity=26.158940397351, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787601, Length=273, Percent_Identity=25.2747252747253, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693105, Length=260, Percent_Identity=23.8461538461538, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=193, Percent_Identity=27.979274611399, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=174, Percent_Identity=28.1609195402299, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790783, Length=164, Percent_Identity=29.8780487804878, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35893; Mature: 35893

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKRLTAFHSVARLKSFSEAVSQTQLSQPTLSRLIKQLEDDLGVILIDRYHRPLQLTEA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCEEHHHH
GRFFYEKIDIILKELDSTISLTQKIAHPPDTLNIGFVPSVLYGLLPTIISRLKQRLPNLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
VHLKDISSFKQIGALKAGEIDVGFGRFAHNDEHIYQLLLRHERYVAALPNTHELAGSHDI
EEHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCHHHCCCCCE
HLSQLQNSRVILYHQTHLPVPQLASASTTEAQNTDPSLISEPLLHLFAKRSALPHHTTSV
EHHHCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
SDIQLALGLVAAGEGITLVPDSLKSVRSEQISYRTLIHEDATSPIYICSLTTPTHPAFTT
HHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
LLEVIYAIYEERGITYRRVI
HHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MQLKRLTAFHSVARLKSFSEAVSQTQLSQPTLSRLIKQLEDDLGVILIDRYHRPLQLTEA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCEEHHHH
GRFFYEKIDIILKELDSTISLTQKIAHPPDTLNIGFVPSVLYGLLPTIISRLKQRLPNLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
VHLKDISSFKQIGALKAGEIDVGFGRFAHNDEHIYQLLLRHERYVAALPNTHELAGSHDI
EEHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCHHHCCCCCE
HLSQLQNSRVILYHQTHLPVPQLASASTTEAQNTDPSLISEPLLHLFAKRSALPHHTTSV
EHHHCCCCEEEEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
SDIQLALGLVAAGEGITLVPDSLKSVRSEQISYRTLIHEDATSPIYICSLTTPTHPAFTT
HHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
LLEVIYAIYEERGITYRRVI
HHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]