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The map label for this gene is prpB [H]

Identifier: 148653003

GI number: 148653003

Start: 1463221

End: 1464114

Strand: Direct

Name: prpB [H]

Synonym: PsycPRwf_1197

Alternate gene names: 148653003

Gene position: 1463221-1464114 (Clockwise)

Preceding gene: 148653002

Following gene: 148653004

Centisome position: 49.12

GC content: 46.53

Gene sequence:

>894_bases
ATGAGCACTTATTCTGCCGGCAAGCGCTTTCGCGAAGCCCTCTTAGCACAAAAATCAAATAACCAGCCGCTACAAATTGT
CGGTACCATCAACGCCTATACCGCCATGATGGCCAAACAAGTTGGTCACAAAGCCATTTACTTATCAGGTGCTGGCGTGG
CAAACGCCTCATACGGCCTACCAGACTTAGGCATGACTAGCCTTGATAACGTATTAGAAGATGCCAGCCGTATTACCAAT
GCGGTGGATACACCACTATTGGTCGATATCGATACCGGTTGGGGCGGTGCATTTAATATCTCGCAAACCATCAAAAAGAT
GGAAAAAGCAGGTGTTGCTGCCGTGCATATTGAAGACCAAGTGGCACAAAAGCGCTGTGGTCACCGTCCAAACAAAGAAA
TCGTTTCAACCGCCGAAATGGTTGACCGTCTTAAAGCGGCGCTTGATGCCAAAGTCGATCCAGACTTTGTGGTGATGGCA
CGTACTGATGCCCTATCGGTCGAAGGCTTAGAAGCAGCTGTTGAACGTGCCGTGGCTTTTGAAGAAGCTGGCGCCGATAT
GATTTTCGCTGAAGCATTAACCGATATCGAGATGTATCGTAAATTCACCGATGTATTAAATATCCCCGTACTCGCCAACA
TGACTGAGTTTGGCCAGACTGACTTATACACCACAGAGCAGCTATTTAATGTGGGCGTCGATATGGTGTTATACCCACTA
TCGGCCTTTCGTGCGATGAACAAAGCGGCGCTAAACGTGTATCAGCATCTATTAAATGATGGTACTCAAGAAGCTGTGGT
CGATACCATGCAGACGCGTATGGAGCTATATGACTTCTTAAATTATCACCAGTTTGAACAAAAGCTAGATGAGCTGTTTG
CTCAAAAGAAGTAA

Upstream 100 bases:

>100_bases
TACCCCTCTTCCTTATTGGCCAATTTAGGGCTATTCTTATTGAAAATACAATCTGCATAAAAACAAGTTATCCCTCCCCC
TTCTATTCAAAGGACTGATT

Downstream 100 bases:

>100_bases
ACATCTTAAATGTGAAAAGTTTTAAGAAGGTATTTTCGAGTAAACCAAATATTTTAATTTAATAATAGTTTGAAGGTTTG
TAGCGTCACAAGGCGGTTGA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSTYSAGKRFREALLAQKSNNQPLQIVGTINAYTAMMAKQVGHKAIYLSGAGVANASYGLPDLGMTSLDNVLEDASRITN
AVDTPLLVDIDTGWGGAFNISQTIKKMEKAGVAAVHIEDQVAQKRCGHRPNKEIVSTAEMVDRLKAALDAKVDPDFVVMA
RTDALSVEGLEAAVERAVAFEEAGADMIFAEALTDIEMYRKFTDVLNIPVLANMTEFGQTDLYTTEQLFNVGVDMVLYPL
SAFRAMNKAALNVYQHLLNDGTQEAVVDTMQTRMELYDFLNYHQFEQKLDELFAQKK

Sequences:

>Translated_297_residues
MSTYSAGKRFREALLAQKSNNQPLQIVGTINAYTAMMAKQVGHKAIYLSGAGVANASYGLPDLGMTSLDNVLEDASRITN
AVDTPLLVDIDTGWGGAFNISQTIKKMEKAGVAAVHIEDQVAQKRCGHRPNKEIVSTAEMVDRLKAALDAKVDPDFVVMA
RTDALSVEGLEAAVERAVAFEEAGADMIFAEALTDIEMYRKFTDVLNIPVLANMTEFGQTDLYTTEQLFNVGVDMVLYPL
SAFRAMNKAALNVYQHLLNDGTQEAVVDTMQTRMELYDFLNYHQFEQKLDELFAQKK
>Mature_296_residues
STYSAGKRFREALLAQKSNNQPLQIVGTINAYTAMMAKQVGHKAIYLSGAGVANASYGLPDLGMTSLDNVLEDASRITNA
VDTPLLVDIDTGWGGAFNISQTIKKMEKAGVAAVHIEDQVAQKRCGHRPNKEIVSTAEMVDRLKAALDAKVDPDFVVMAR
TDALSVEGLEAAVERAVAFEEAGADMIFAEALTDIEMYRKFTDVLNIPVLANMTEFGQTDLYTTEQLFNVGVDMVLYPLS
AFRAMNKAALNVYQHLLNDGTQEAVVDTMQTRMELYDFLNYHQFEQKLDELFAQKK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=298, Percent_Identity=61.0738255033557, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790445, Length=266, Percent_Identity=28.9473684210526, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71982926, Length=194, Percent_Identity=31.9587628865979, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17561814, Length=194, Percent_Identity=31.9587628865979, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32735; Mature: 32603

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTYSAGKRFREALLAQKSNNQPLQIVGTINAYTAMMAKQVGHKAIYLSGAGVANASYGL
CCCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
PDLGMTSLDNVLEDASRITNAVDTPLLVDIDTGWGGAFNISQTIKKMEKAGVAAVHIEDQ
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEEHHH
VAQKRCGHRPNKEIVSTAEMVDRLKAALDAKVDPDFVVMARTDALSVEGLEAAVERAVAF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
EEAGADMIFAEALTDIEMYRKFTDVLNIPVLANMTEFGQTDLYTTEQLFNVGVDMVLYPL
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCHHHHHHHHHCHHHHHHHH
SAFRAMNKAALNVYQHLLNDGTQEAVVDTMQTRMELYDFLNYHQFEQKLDELFAQKK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STYSAGKRFREALLAQKSNNQPLQIVGTINAYTAMMAKQVGHKAIYLSGAGVANASYGL
CCCHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCEEEEECCCCCCCCCCC
PDLGMTSLDNVLEDASRITNAVDTPLLVDIDTGWGGAFNISQTIKKMEKAGVAAVHIEDQ
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEEHHH
VAQKRCGHRPNKEIVSTAEMVDRLKAALDAKVDPDFVVMARTDALSVEGLEAAVERAVAF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
EEAGADMIFAEALTDIEMYRKFTDVLNIPVLANMTEFGQTDLYTTEQLFNVGVDMVLYPL
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCHHHHHHHHHCHHHHHHHH
SAFRAMNKAALNVYQHLLNDGTQEAVVDTMQTRMELYDFLNYHQFEQKLDELFAQKK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]