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The map label for this gene is potD [H]

Identifier: 148652871

GI number: 148652871

Start: 1284135

End: 1285259

Strand: Direct

Name: potD [H]

Synonym: PsycPRwf_1064

Alternate gene names: 148652871

Gene position: 1284135-1285259 (Clockwise)

Preceding gene: 148652870

Following gene: 148652872

Centisome position: 43.11

GC content: 46.22

Gene sequence:

>1125_bases
ATGGCTAATAACAACTTATTCTCACGCCGACGTTTTTTGGGAATGACAGGTGCAGGTTTGGTTGGCTTATCGGGACTGGG
TCTAGCCGGCTGTCAGCGCTCTAATACCGACACTGATACCGCCGATTTGGCCAATACCGCGCGTGGTAACGTGGTGAAGG
TGTATAACTGGACCGAGTACATTTCAGACAGTGTGCTAAAAGGCTTTACCCAAGAGACGGGCATCGAGGTCATTTATAGT
ACCTTTGACTCCAATGAGGCCATGTATGCTAAGCTAAAACTGATGAATGGCAGCGGCGATTATGATGTGATATTTCCAGG
TACAGACTTTGTCGATAAGATGCGCAAAGAGAGTATGCTTGAGGTCATAGATCATAATAAGCTCAGTAATTTTGGTAATT
TAAGTCCTACTTTCTTAGATGCCTCGTTTGACCCGCAAAACAAATTTAGTGTGCCCTATCTGTGGGGCTCATCTGGGATT
GCAGTCAATAAATCCCGTATTGATATTAACAGTATCACCTCTTGGAATGACTTATGGCGTCCTGAATTTGAGGGCCGGGT
GATGCTAATGAATGATTTGCGTGATGTGTTTACCATGGGGCTATTAACCTTAGGTTATCCCGCCACCACTCATGACCCTA
AGCACATTGAAGCGGCATACCAAAAGCTGTCAAAGCTCATGCCCAACGTGCGCACCTTTAACTCAGATGCACCGCGTATG
CCGTTTATGGAAGGGGAGACGTATCTGGGGCTTGCCTGGAATGGTGAGGTAATTATGGCGCAAGATCAGGGCATGCCTGA
GCTTGATTTTGTGTATCCCAAAGAAGGGGTGATTATGTGGATGGATAACATGTCCATTCCCAAAAATGCCAAAAACCGCG
AAAATGCCTACGCCTTTATCGACTATCTACTGCGCCCAGACAATGCCGCCATTATTAGTGAAGAGATCGGTTACGGCTCA
CCTAATGAAAGCGCCAAGAAGCTGATGCCTGCAGATATTGCCAATAACCAAATTATCTATCCTCCCGATGACTTACTTGG
TCACGCTTTGTTCCGAGAAGATGTTGGCGATACCACCATGGCCTTGTATCAAAAATACTGGGACCGACTCAAGGTCGATA
TGTAG

Upstream 100 bases:

>100_bases
GCCTGAGGTCAATGCGGTGGGTACCTTATTATTGGCCATTTCACTGGTGGGCTTGGTCATCTCTCAATTACTACTTCTTA
AAAAACGTTAAGGATTTATG

Downstream 100 bases:

>100_bases
GCCGGCATAATGAGCGCTTTATCCCATCTGCTTAATCACCATTGGCGCTTATTAGTCAGCTTACTAATAGGTGCCCTGAT
GGGTGGTGTGGTCCACCTAA

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MANNNLFSRRRFLGMTGAGLVGLSGLGLAGCQRSNTDTDTADLANTARGNVVKVYNWTEYISDSVLKGFTQETGIEVIYS
TFDSNEAMYAKLKLMNGSGDYDVIFPGTDFVDKMRKESMLEVIDHNKLSNFGNLSPTFLDASFDPQNKFSVPYLWGSSGI
AVNKSRIDINSITSWNDLWRPEFEGRVMLMNDLRDVFTMGLLTLGYPATTHDPKHIEAAYQKLSKLMPNVRTFNSDAPRM
PFMEGETYLGLAWNGEVIMAQDQGMPELDFVYPKEGVIMWMDNMSIPKNAKNRENAYAFIDYLLRPDNAAIISEEIGYGS
PNESAKKLMPADIANNQIIYPPDDLLGHALFREDVGDTTMALYQKYWDRLKVDM

Sequences:

>Translated_374_residues
MANNNLFSRRRFLGMTGAGLVGLSGLGLAGCQRSNTDTDTADLANTARGNVVKVYNWTEYISDSVLKGFTQETGIEVIYS
TFDSNEAMYAKLKLMNGSGDYDVIFPGTDFVDKMRKESMLEVIDHNKLSNFGNLSPTFLDASFDPQNKFSVPYLWGSSGI
AVNKSRIDINSITSWNDLWRPEFEGRVMLMNDLRDVFTMGLLTLGYPATTHDPKHIEAAYQKLSKLMPNVRTFNSDAPRM
PFMEGETYLGLAWNGEVIMAQDQGMPELDFVYPKEGVIMWMDNMSIPKNAKNRENAYAFIDYLLRPDNAAIISEEIGYGS
PNESAKKLMPADIANNQIIYPPDDLLGHALFREDVGDTTMALYQKYWDRLKVDM
>Mature_373_residues
ANNNLFSRRRFLGMTGAGLVGLSGLGLAGCQRSNTDTDTADLANTARGNVVKVYNWTEYISDSVLKGFTQETGIEVIYST
FDSNEAMYAKLKLMNGSGDYDVIFPGTDFVDKMRKESMLEVIDHNKLSNFGNLSPTFLDASFDPQNKFSVPYLWGSSGIA
VNKSRIDINSITSWNDLWRPEFEGRVMLMNDLRDVFTMGLLTLGYPATTHDPKHIEAAYQKLSKLMPNVRTFNSDAPRMP
FMEGETYLGLAWNGEVIMAQDQGMPELDFVYPKEGVIMWMDNMSIPKNAKNRENAYAFIDYLLRPDNAAIISEEIGYGSP
NESAKKLMPADIANNQIIYPPDDLLGHALFREDVGDTTMALYQKYWDRLKVDM

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=329, Percent_Identity=49.8480243161094, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI1787078, Length=345, Percent_Identity=31.8840579710145, Blast_Score=183, Evalue=1e-47,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42070; Mature: 41939

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNNLFSRRRFLGMTGAGLVGLSGLGLAGCQRSNTDTDTADLANTARGNVVKVYNWTEY
CCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEHHHH
ISDSVLKGFTQETGIEVIYSTFDSNEAMYAKLKLMNGSGDYDVIFPGTDFVDKMRKESML
HHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHH
EVIDHNKLSNFGNLSPTFLDASFDPQNKFSVPYLWGSSGIAVNKSRIDINSITSWNDLWR
HHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCEEEECCEEEECCCCCHHHCCC
PEFEGRVMLMNDLRDVFTMGLLTLGYPATTHDPKHIEAAYQKLSKLMPNVRTFNSDAPRM
CCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCC
PFMEGETYLGLAWNGEVIMAQDQGMPELDFVYPKEGVIMWMDNMSIPKNAKNRENAYAFI
CCCCCCEEEEEEECCCEEEECCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHEEE
DYLLRPDNAAIISEEIGYGSPNESAKKLMPADIANNQIIYPPDDLLGHALFREDVGDTTM
HHHCCCCCCEEEEHHCCCCCCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHCCCHHHH
ALYQKYWDRLKVDM
HHHHHHHHHHCCCC
>Mature Secondary Structure 
ANNNLFSRRRFLGMTGAGLVGLSGLGLAGCQRSNTDTDTADLANTARGNVVKVYNWTEY
CCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEHHHH
ISDSVLKGFTQETGIEVIYSTFDSNEAMYAKLKLMNGSGDYDVIFPGTDFVDKMRKESML
HHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHH
EVIDHNKLSNFGNLSPTFLDASFDPQNKFSVPYLWGSSGIAVNKSRIDINSITSWNDLWR
HHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCEEEECCEEEECCCCCHHHCCC
PEFEGRVMLMNDLRDVFTMGLLTLGYPATTHDPKHIEAAYQKLSKLMPNVRTFNSDAPRM
CCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCC
PFMEGETYLGLAWNGEVIMAQDQGMPELDFVYPKEGVIMWMDNMSIPKNAKNRENAYAFI
CCCCCCEEEEEEECCCEEEECCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCHHHEEE
DYLLRPDNAAIISEEIGYGSPNESAKKLMPADIANNQIIYPPDDLLGHALFREDVGDTTM
HHHCCCCCCEEEEHHCCCCCCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHCCCHHHH
ALYQKYWDRLKVDM
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]