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The map label for this gene is mhpC [H]

Identifier: 148652800

GI number: 148652800

Start: 1179617

End: 1180654

Strand: Direct

Name: mhpC [H]

Synonym: PsycPRwf_0993

Alternate gene names: 148652800

Gene position: 1179617-1180654 (Clockwise)

Preceding gene: 148652799

Following gene: 148652801

Centisome position: 39.6

GC content: 47.01

Gene sequence:

>1038_bases
ATGGATAAGCATTTGACGTTTAATAAGACACTTTTAGTCAAGATGGGTTTGGCGGGTGTGCTGGCAACCTCTGCGGCAGC
ACTTGTCATCGGCCGCATCTGGAAGCCAGATTTGCAGGTCTCTAAGCTTAAGCATTGGGAGCTGCCGACCAGCTTTTATG
TCGATGTAGAAGGATTACAGGTGCATGTGGCCAAGTCTGAGACATCTGATAACTTAGCGGCTACTAATAACTGTGAAGCG
CAAAGCGACGCTGAAACTTTGTTACTAATCCATGGCACGTCAGCCAGCTTGCATACTTGGGATGGCTGGACTGAGGCGCT
TAAAGAACAATATTGCGTGGTACGCTTAGACTTGCCAGCCTTTGGGTTAACAGGCCCTTATGCTGATGATACCAAGCCCT
ATAGTTTAGATAATTATGTTGATACTGTCATTAAAGTGATGGATAAGCTTGATATCAAGCGAGCGACCATTGCCGGTAAT
TCGCTTGGCGGCGGCATTGCTTGGCTAACTGCTTTAATGCACCCTGAGCGGATTGACCGCTTGATATTGGTTGATGCATC
GGGCTTTAAATTTACCCCTAAACGCATGCCCATTGGCTTTAAACTGGCACAGTCTCCTGTACTAGATGGTCTTACCGAGC
ATGTACTCCCCAAAAGCATGGTGCGCAGTAGTGTGCAGAGTGTTTATACAGATAAATCCAAGGTAAGCGATGACTTAGTG
AACCGTTATTATGAGCTCAGCCGCCGTGCAGGCAATCGTAAAGCCTTGACCAGACGCATGCGCGAGGGGTTATATCAAGA
TGAAGTCAAGCGCCTGGGTGAGATTACGCAGCCCACACTGATTATCTGGGGCGCACAAGATGAATTAATTCCTATTGAAA
GCGCTTATAAATTTAAGGCTGCCATACCCAATAGCCAATTGGTGGTATTTGATCACCTAGGTCATGTGCCGCAAGAGGAA
GACCCGAAAGCCACAGTGGCTGTGGTGAAGCAGTTCTTGCGAGACACCAAATCTGACTATACTAAGTCGTTTAACTAA

Upstream 100 bases:

>100_bases
TATCTTGTAATAACCTGAATGATAAGGATTGAGCTTAATCAGCAAAAACCAATCGTTAAGACCAGTAATCAAAGATAAAT
TAACCAAGGATAGGTGTTCA

Downstream 100 bases:

>100_bases
ATAAATCATCCAGTATTGAGTTAAAGGGGCAGAGTATGACGACACTGTTTATGGTGCAGCTTGGCGCCACGCCAAAGGGT
CGGCTGATTGAACAGCACGA

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MDKHLTFNKTLLVKMGLAGVLATSAAALVIGRIWKPDLQVSKLKHWELPTSFYVDVEGLQVHVAKSETSDNLAATNNCEA
QSDAETLLLIHGTSASLHTWDGWTEALKEQYCVVRLDLPAFGLTGPYADDTKPYSLDNYVDTVIKVMDKLDIKRATIAGN
SLGGGIAWLTALMHPERIDRLILVDASGFKFTPKRMPIGFKLAQSPVLDGLTEHVLPKSMVRSSVQSVYTDKSKVSDDLV
NRYYELSRRAGNRKALTRRMREGLYQDEVKRLGEITQPTLIIWGAQDELIPIESAYKFKAAIPNSQLVVFDHLGHVPQEE
DPKATVAVVKQFLRDTKSDYTKSFN

Sequences:

>Translated_345_residues
MDKHLTFNKTLLVKMGLAGVLATSAAALVIGRIWKPDLQVSKLKHWELPTSFYVDVEGLQVHVAKSETSDNLAATNNCEA
QSDAETLLLIHGTSASLHTWDGWTEALKEQYCVVRLDLPAFGLTGPYADDTKPYSLDNYVDTVIKVMDKLDIKRATIAGN
SLGGGIAWLTALMHPERIDRLILVDASGFKFTPKRMPIGFKLAQSPVLDGLTEHVLPKSMVRSSVQSVYTDKSKVSDDLV
NRYYELSRRAGNRKALTRRMREGLYQDEVKRLGEITQPTLIIWGAQDELIPIESAYKFKAAIPNSQLVVFDHLGHVPQEE
DPKATVAVVKQFLRDTKSDYTKSFN
>Mature_345_residues
MDKHLTFNKTLLVKMGLAGVLATSAAALVIGRIWKPDLQVSKLKHWELPTSFYVDVEGLQVHVAKSETSDNLAATNNCEA
QSDAETLLLIHGTSASLHTWDGWTEALKEQYCVVRLDLPAFGLTGPYADDTKPYSLDNYVDTVIKVMDKLDIKRATIAGN
SLGGGIAWLTALMHPERIDRLILVDASGFKFTPKRMPIGFKLAQSPVLDGLTEHVLPKSMVRSSVQSVYTDKSKVSDDLV
NRYYELSRRAGNRKALTRRMREGLYQDEVKRLGEITQPTLIIWGAQDELIPIESAYKFKAAIPNSQLVVFDHLGHVPQEE
DPKATVAVVKQFLRDTKSDYTKSFN

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=273, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566936, Length=300, Percent_Identity=24.3333333333333, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 38474; Mature: 38474

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKHLTFNKTLLVKMGLAGVLATSAAALVIGRIWKPDLQVSKLKHWELPTSFYVDVEGLQ
CCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCEE
VHVAKSETSDNLAATNNCEAQSDAETLLLIHGTSASLHTWDGWTEALKEQYCVVRLDLPA
EEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCEEEEEECCCC
FGLTGPYADDTKPYSLDNYVDTVIKVMDKLDIKRATIAGNSLGGGIAWLTALMHPERIDR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHCHHHCCE
LILVDASGFKFTPKRMPIGFKLAQSPVLDGLTEHVLPKSMVRSSVQSVYTDKSKVSDDLV
EEEEECCCCEECCCCCCCCCEECCCHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHH
NRYYELSRRAGNRKALTRRMREGLYQDEVKRLGEITQPTLIIWGAQDELIPIESAYKFKA
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCEEECCCCCEEEE
AIPNSQLVVFDHLGHVPQEEDPKATVAVVKQFLRDTKSDYTKSFN
ECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKHLTFNKTLLVKMGLAGVLATSAAALVIGRIWKPDLQVSKLKHWELPTSFYVDVEGLQ
CCCCEECCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEECCEE
VHVAKSETSDNLAATNNCEAQSDAETLLLIHGTSASLHTWDGWTEALKEQYCVVRLDLPA
EEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCEEEEEECCCC
FGLTGPYADDTKPYSLDNYVDTVIKVMDKLDIKRATIAGNSLGGGIAWLTALMHPERIDR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHCHHHCCE
LILVDASGFKFTPKRMPIGFKLAQSPVLDGLTEHVLPKSMVRSSVQSVYTDKSKVSDDLV
EEEEECCCCEECCCCCCCCCEECCCHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHH
NRYYELSRRAGNRKALTRRMREGLYQDEVKRLGEITQPTLIIWGAQDELIPIESAYKFKA
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCEEECCCCCEEEE
AIPNSQLVVFDHLGHVPQEEDPKATVAVVKQFLRDTKSDYTKSFN
ECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA