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The map label for this gene is ispF [H]

Identifier: 148652769

GI number: 148652769

Start: 1149173

End: 1149730

Strand: Direct

Name: ispF [H]

Synonym: PsycPRwf_0962

Alternate gene names: 148652769

Gene position: 1149173-1149730 (Clockwise)

Preceding gene: 148652766

Following gene: 148652770

Centisome position: 38.58

GC content: 44.8

Gene sequence:

>558_bases
ATGCTAAAATTTGGGCTAAATTATAACACTGATAGGCTGATAATGATAAGAATTGGACAAGGTATTGATGTACACGCGTT
TTATGACAAAAAGCTGGACGCACAAAGCGCCAAAAGTGAGCCCAATCAATTTGTTACCCTAGCGGGCGTGAAGATTGAGC
ACAGCCACAGTCTGCTGGCTCACTCTGATGGCGATGTGGTATTGCATGCACTTGCCGATGCGCTTTTGGGCGCTTTAGCA
CTGGGGGATATCGGTCAGCATTTCCCAGATACTGATGATGCCAATTCAGGACTAGATTCACGCGTGCTGCTGCGCCATGT
TTATGATTTGATTCTCCAAAAAGGCTACAGCTTAGTCAATGCCGATATTACGGTCATGTGTGAGCGTCCTAAGCTTGCCA
AACACAATATGGCGATGCGCCTTAATATCGCTCAAGACTTACAGACTCACGTTGATAATATTAGTGTTAAAGCCACCACT
ACTGAGAAGTTAGGTTTTACGGGTCGTCAAGAGGGTATTATGACCAGTGCTGTGGTGTTATTAACAAAGCAGGTGTAA

Upstream 100 bases:

>100_bases
CGAGTCTGGGCAGGATTGGGCACATCGCTTAGGCGTAGGGCGGGCAAATTATAGGCGTTATTATCAAAACCAGTAGATGT
CGTCATAAGTATCAGAAACT

Downstream 100 bases:

>100_bases
GCTAATTTTTTAGCAAAAGCTGAATTGAGATGACAGATAAGATGCATTTTCGTTGTATTTTTTGTTACTATCCCCATAAA
TACTATAATTACCTATGAAT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MLKFGLNYNTDRLIMIRIGQGIDVHAFYDKKLDAQSAKSEPNQFVTLAGVKIEHSHSLLAHSDGDVVLHALADALLGALA
LGDIGQHFPDTDDANSGLDSRVLLRHVYDLILQKGYSLVNADITVMCERPKLAKHNMAMRLNIAQDLQTHVDNISVKATT
TEKLGFTGRQEGIMTSAVVLLTKQV

Sequences:

>Translated_185_residues
MLKFGLNYNTDRLIMIRIGQGIDVHAFYDKKLDAQSAKSEPNQFVTLAGVKIEHSHSLLAHSDGDVVLHALADALLGALA
LGDIGQHFPDTDDANSGLDSRVLLRHVYDLILQKGYSLVNADITVMCERPKLAKHNMAMRLNIAQDLQTHVDNISVKATT
TEKLGFTGRQEGIMTSAVVLLTKQV
>Mature_185_residues
MLKFGLNYNTDRLIMIRIGQGIDVHAFYDKKLDAQSAKSEPNQFVTLAGVKIEHSHSLLAHSDGDVVLHALADALLGALA
LGDIGQHFPDTDDANSGLDSRVLLRHVYDLILQKGYSLVNADITVMCERPKLAKHNMAMRLNIAQDLQTHVDNISVKATT
TEKLGFTGRQEGIMTSAVVLLTKQV

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=168, Percent_Identity=53.5714285714286, Blast_Score=168, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 20274; Mature: 20274

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFGLNYNTDRLIMIRIGQGIDVHAFYDKKLDAQSAKSEPNQFVTLAGVKIEHSHSLLA
CEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCHHCCCCCCEEEEEEEEEEECCCCEEE
HSDGDVVLHALADALLGALALGDIGQHFPDTDDANSGLDSRVLLRHVYDLILQKGYSLVN
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEC
ADITVMCERPKLAKHNMAMRLNIAQDLQTHVDNISVKATTTEKLGFTGRQEGIMTSAVVL
CCEEEEECCCCCCCCCEEEEEEHHHHHHHHHCCEEEEEECHHHCCCCCCCCCHHHHHEEE
LTKQV
EEECC
>Mature Secondary Structure
MLKFGLNYNTDRLIMIRIGQGIDVHAFYDKKLDAQSAKSEPNQFVTLAGVKIEHSHSLLA
CEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCHHCCCCCCEEEEEEEEEEECCCCEEE
HSDGDVVLHALADALLGALALGDIGQHFPDTDDANSGLDSRVLLRHVYDLILQKGYSLVN
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEC
ADITVMCERPKLAKHNMAMRLNIAQDLQTHVDNISVKATTTEKLGFTGRQEGIMTSAVVL
CCEEEEECCCCCCCCCEEEEEEHHHHHHHHHCCEEEEEECHHHCCCCCCCCCHHHHHEEE
LTKQV
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA