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The map label for this gene is purF [H]

Identifier: 148652743

GI number: 148652743

Start: 1118303

End: 1119838

Strand: Direct

Name: purF [H]

Synonym: PsycPRwf_0936

Alternate gene names: 148652743

Gene position: 1118303-1119838 (Clockwise)

Preceding gene: 148652742

Following gene: 148652744

Centisome position: 37.54

GC content: 45.9

Gene sequence:

>1536_bases
ATGTGTGGAGTTGTAGGTGTTGCAGCACATGAACCAGTCAACCAGATGTTGTATGACGCATTAACTGTACTTCAACACCG
TGGGCAAGATGCCGCTGGTATCGTGACCATGAAAGATGGACGCTTTTTTCTGCGTAAAGACAATGGTATGGTGCGTGATG
TGTTTATGACACGTCATATGTTGCGACTTGTGGGTGAGTTTGGTGTGGGTCATGTCAGATACCCAACAGCAGGCACTTCA
AGTAGTGCAGAGGCGCAACCTTTTTATGTAAACTCTCCTTATGGCATTACACTTGCACATAATGGTAACTTGACCAATGC
AGAAACCTTGGCAAAAGAGCTGTATCAAGATGACCTACGTCATCTAAATACCAACTCTGACTCAGAGGTATTATTAAACG
TATTGGCGCATGAAATTCAGGCATTAGGCAAAACTCAGCCTACGCCCGATGACATTTTTGATGCGATCAGCTCAATGTAT
AAGCGTATCGAAGGCGCTTATGCTGTAGTGGCGCTAATTACTGGTCAAGGCCTGATTGCTTTTAGAGACCCTAATGGTAT
TCGTCCACTGATTTACGGCGAGCGTGAAGCTGCTGATGGCCGCATCGAATACATGGTGGCTTCAGAGTCAGTGGCTCTGA
CTGGATCAGGATTCAAAGTGGTTCGTGATATCAAGCCTGGTGAAGCCATCTTTATTGACCTTGATAATAAGATGCACACC
CGTCAATGTACGGAGCAAAAAGAGTACACCCCTTGTATGTTTGAGTATGTATATTTTGCGCGTCCAGATTCTATTATGGA
CAACATCTCAGTATACAAAGCGCGTCTGCGTATGGGTGAGAAGTTGGCTCAGAAGATTTTGGATGAGTGGGGCAATGAGC
ACGATATCGACGTGGTTATTCCTATCCCAGATACGTCACGTACCTCAGCGATGGAGCTGGCGCTTAAGCTTAATGTGAAG
TACCGCGAAGGCTTTATGAAAAACCGCTATATCGGCCGCACCTTTATTATGCCCGGTCAGCAGCAGCGCAAAAAATCGGT
GCGCCAAAAGCTAAATGCAGTACCACTTGAATTTAAAAATAAAAATGTCTTATTGGTCGATGACTCTATCGTACGTGGTA
CGACCTGTCATGAGATTATCCAAATGGCGCGTGATGCCGGTGCCAATAAGGTGTTTTTTGCCAGCGCCGCGCCACCGGTT
AAATTCCCGAATGTCTATGGCATTGATATGCCAGTGCGTCATGAGCTGATTGCATCAGGTCATACGGTAGAAGAGGTTCG
TGACATTATCGGTGCTGACCGTTTGATTTTCCAAGACCTAGATGACTTGATTGAAGCGGTACAAGATACTAAGCACAGCA
AGGTAGAAGGTTTTGATTGTGCGGTATTCGATGGTAAATACATCACAGGTACCATTACCCCTGAGTATTTAGACTACCTG
CAAGAGCAGCGCAACGATCAGGCCAAAAGCAAGGCAGGGGGAGTGGAAGTCATTGCAGATACGCCAGTGGACATGACTGG
GGTACAAGAAAACTAA

Upstream 100 bases:

>100_bases
GTTGATAACCAAAAGCTGAGTGTTTTCAGCGATGAAGTGTAGTTTAGCCACCACTAAATGAATCTTTTAACCAGTAAAGA
CTATTTTAAAAGGATAGAGT

Downstream 100 bases:

>100_bases
GCGAGGCGTTTAAGATCATTATCATCTGATAATATAAAGCAGATTGATGTTAACTGAAAAATTGCCCGCCAAGCTCACCA
TTAGTTTGTTATGATAAAAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MCGVVGVAAHEPVNQMLYDALTVLQHRGQDAAGIVTMKDGRFFLRKDNGMVRDVFMTRHMLRLVGEFGVGHVRYPTAGTS
SSAEAQPFYVNSPYGITLAHNGNLTNAETLAKELYQDDLRHLNTNSDSEVLLNVLAHEIQALGKTQPTPDDIFDAISSMY
KRIEGAYAVVALITGQGLIAFRDPNGIRPLIYGEREAADGRIEYMVASESVALTGSGFKVVRDIKPGEAIFIDLDNKMHT
RQCTEQKEYTPCMFEYVYFARPDSIMDNISVYKARLRMGEKLAQKILDEWGNEHDIDVVIPIPDTSRTSAMELALKLNVK
YREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNAVPLEFKNKNVLLVDDSIVRGTTCHEIIQMARDAGANKVFFASAAPPV
KFPNVYGIDMPVRHELIASGHTVEEVRDIIGADRLIFQDLDDLIEAVQDTKHSKVEGFDCAVFDGKYITGTITPEYLDYL
QEQRNDQAKSKAGGVEVIADTPVDMTGVQEN

Sequences:

>Translated_511_residues
MCGVVGVAAHEPVNQMLYDALTVLQHRGQDAAGIVTMKDGRFFLRKDNGMVRDVFMTRHMLRLVGEFGVGHVRYPTAGTS
SSAEAQPFYVNSPYGITLAHNGNLTNAETLAKELYQDDLRHLNTNSDSEVLLNVLAHEIQALGKTQPTPDDIFDAISSMY
KRIEGAYAVVALITGQGLIAFRDPNGIRPLIYGEREAADGRIEYMVASESVALTGSGFKVVRDIKPGEAIFIDLDNKMHT
RQCTEQKEYTPCMFEYVYFARPDSIMDNISVYKARLRMGEKLAQKILDEWGNEHDIDVVIPIPDTSRTSAMELALKLNVK
YREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNAVPLEFKNKNVLLVDDSIVRGTTCHEIIQMARDAGANKVFFASAAPPV
KFPNVYGIDMPVRHELIASGHTVEEVRDIIGADRLIFQDLDDLIEAVQDTKHSKVEGFDCAVFDGKYITGTITPEYLDYL
QEQRNDQAKSKAGGVEVIADTPVDMTGVQEN
>Mature_511_residues
MCGVVGVAAHEPVNQMLYDALTVLQHRGQDAAGIVTMKDGRFFLRKDNGMVRDVFMTRHMLRLVGEFGVGHVRYPTAGTS
SSAEAQPFYVNSPYGITLAHNGNLTNAETLAKELYQDDLRHLNTNSDSEVLLNVLAHEIQALGKTQPTPDDIFDAISSMY
KRIEGAYAVVALITGQGLIAFRDPNGIRPLIYGEREAADGRIEYMVASESVALTGSGFKVVRDIKPGEAIFIDLDNKMHT
RQCTEQKEYTPCMFEYVYFARPDSIMDNISVYKARLRMGEKLAQKILDEWGNEHDIDVVIPIPDTSRTSAMELALKLNVK
YREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNAVPLEFKNKNVLLVDDSIVRGTTCHEIIQMARDAGANKVFFASAAPPV
KFPNVYGIDMPVRHELIASGHTVEEVRDIIGADRLIFQDLDDLIEAVQDTKHSKVEGFDCAVFDGKYITGTITPEYLDYL
QEQRNDQAKSKAGGVEVIADTPVDMTGVQEN

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=482, Percent_Identity=36.5145228215768, Blast_Score=259, Evalue=5e-69,
Organism=Escherichia coli, GI1788651, Length=491, Percent_Identity=61.5071283095723, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1790167, Length=216, Percent_Identity=27.3148148148148, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=31.8840579710145, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6323958, Length=499, Percent_Identity=50.9018036072144, Blast_Score=499, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=34.4897959183673, Blast_Score=262, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24659604, Length=487, Percent_Identity=34.4969199178645, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI28573187, Length=514, Percent_Identity=33.852140077821, Blast_Score=248, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 57039; Mature: 57039

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVVGVAAHEPVNQMLYDALTVLQHRGQDAAGIVTMKDGRFFLRKDNGMVRDVFMTRHM
CCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHH
LRLVGEFGVGHVRYPTAGTSSSAEAQPFYVNSPYGITLAHNGNLTNAETLAKELYQDDLR
HHHHHHHCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HLNTNSDSEVLLNVLAHEIQALGKTQPTPDDIFDAISSMYKRIEGAYAVVALITGQGLIA
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEE
FRDPNGIRPLIYGEREAADGRIEYMVASESVALTGSGFKVVRDIKPGEAIFIDLDNKMHT
EECCCCCCCEEECCCCCCCCEEEEEEECCCEEEECCCCEEEEECCCCCEEEEECCCCHHH
RQCTEQKEYTPCMFEYVYFARPDSIMDNISVYKARLRMGEKLAQKILDEWGNEHDIDVVI
HHHHHHCCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PIPDTSRTSAMELALKLNVKYREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNAVPLEFKN
ECCCCCCCHHEEEEEEEEEHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEECC
KNVLLVDDSIVRGTTCHEIIQMARDAGANKVFFASAAPPVKFPNVYGIDMPVRHELIASG
CEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHCC
HTVEEVRDIIGADRLIFQDLDDLIEAVQDTKHSKVEGFDCAVFDGKYITGTITPEYLDYL
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEECCHHHHHHH
QEQRNDQAKSKAGGVEVIADTPVDMTGVQEN
HHHHCHHHHHHCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MCGVVGVAAHEPVNQMLYDALTVLQHRGQDAAGIVTMKDGRFFLRKDNGMVRDVFMTRHM
CCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHH
LRLVGEFGVGHVRYPTAGTSSSAEAQPFYVNSPYGITLAHNGNLTNAETLAKELYQDDLR
HHHHHHHCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HLNTNSDSEVLLNVLAHEIQALGKTQPTPDDIFDAISSMYKRIEGAYAVVALITGQGLIA
HCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEE
FRDPNGIRPLIYGEREAADGRIEYMVASESVALTGSGFKVVRDIKPGEAIFIDLDNKMHT
EECCCCCCCEEECCCCCCCCEEEEEEECCCEEEECCCCEEEEECCCCCEEEEECCCCHHH
RQCTEQKEYTPCMFEYVYFARPDSIMDNISVYKARLRMGEKLAQKILDEWGNEHDIDVVI
HHHHHHCCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PIPDTSRTSAMELALKLNVKYREGFMKNRYIGRTFIMPGQQQRKKSVRQKLNAVPLEFKN
ECCCCCCCHHEEEEEEEEEHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEECC
KNVLLVDDSIVRGTTCHEIIQMARDAGANKVFFASAAPPVKFPNVYGIDMPVRHELIASG
CEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHCC
HTVEEVRDIIGADRLIFQDLDDLIEAVQDTKHSKVEGFDCAVFDGKYITGTITPEYLDYL
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEECCHHHHHHH
QEQRNDQAKSKAGGVEVIADTPVDMTGVQEN
HHHHCHHHHHHCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]