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The map label for this gene is topA [H]

Identifier: 148652205

GI number: 148652205

Start: 474310

End: 476964

Strand: Direct

Name: topA [H]

Synonym: PsycPRwf_0393

Alternate gene names: 148652205

Gene position: 474310-476964 (Clockwise)

Preceding gene: 148652204

Following gene: 148652208

Centisome position: 15.92

GC content: 48.02

Gene sequence:

>2655_bases
ATGGCTAAAACCACAGCTAAAAAAACAACCTCGACCCCCGCGGCCAGTGCAAAAGGCAAATCACTGGTCATTGTGGAGTC
TCCTGCCAAAGCAAAAACCATCAACAAATATTTGGGTGATGACTTTATTGTACGCTCAAGTATTGGTCATGTGCGTGACT
TGCCCACCAGCAGTGGCGTTAAGAAAAAAGCAACCAAGACGGATGAAAAGCTAAGCAAAGAAGACAAAGCGCAGCAGGCT
TTGGTACGTAGAATGGGTATTGACCCTGAGCACGGCTGGAAAGCTGTGTATGAAGTGCTGCCTAATAAAACCAAAGTGAT
TAAAGAGCTTAAAGCCTTGGCCAAAGATGCCGATAAGATTTATCTGGCAACGGATTTGGATAGAGAAGGGGAAGCGATTG
CTTGGCATTTGCGTGAGGTTATCGGCGGAGATGACAGCAAGTATCAGCGTGTGGTGTTTAACGAGATTACCAAATCGGCC
ATTCAAAATGCATTTAAAGAGCCCGGTAAGCTTGATATTGACCGGGTGAATGCGCAGCAAGCACGCCGCTTCTTAGACAG
AGTGGTTGGGTTTATGGTCTCGCCGCTACTGTGGCAAAAAATTGCCCGTGGCTTATCGGCTGGGCGAGTACAGTCAGTAG
CCACTCGGCTTGTGGTCGAGCGTGAACGTGAAATCCGTGCCTTTATTCCAGAAGAGTATTGGCAAATTCACGCCGATACC
ACCATGAAAGGTAGCGAGAATGCCAATGTGGCTGCAGATACTCCCATTCGCTTAGAGGCCGTCAAACAGGCAGGCAAAGT
ACTCAAGCTTGGCAACAAAGCGCAGACTGATGCCGTACTGGCCGTATTAAAACCGGCCACCTTCGTGGTCAAAGAGCGTG
AAGAGAAGCCCACTCAATCTAAGCCGAGTGCGCCTTTTATTACCTCTACTTTACAGCAAGCGGCCAGCACACGTTTGGGC
TTTCCTGTCAAAAAGACCATGATGCTAGCTCAGCGCTTGTACGAAGCTGGTCACATTACTTATATGCGTACCGATTCAAC
CTTCTTATCCAATGATGCCTTGGCCAGTGTGCGCGGCTTTATTGAAGAGAATTACGGCTCTAAATACTTGCCAGAAAAGC
CTAATTTTTATGGCAGCAAACAAAACGCACAAGAGGCTCACGAAGCCATTCGTCCGACCAATGCCAATTTAAAATCCACC
CAACTCAAAGGGGTGGATCGTGATGCGATTCGCTTATATGAGCTGATTTGGCGTCAGTTTGTGGCCTGTCAGATGCTACC
GGCAAAATATAAGTCGGTTAATCTATTGGTAGAGGCGGGCGATGTTGAGCTAAAAGCCCGCGGTCGCACCATGACCTTTG
ACGGCTTTACCAAGGTTATGCCACCTGCCAAAACCGATGACACCTTATTGCCTGATGTCAAACAAGGCGATGAGCTTAAT
TTAATAAAACTAGATCCCAGTCAGCACTTCACTAAGCCGCCAGCGCGTTATACGGAAGCAAGCTTAGTTAAAGAGCTTGA
GAAAAAAGGTATCGGCCGTCCATCAACTTATGCCTCTATCATCTCAACCATTCAAGACAGAGGGTATGTTCGCTTAGAAA
ACAAGCGTCTGTATGCTGAGAAGATGGGTGATATTGTCACCGACCGTCTAACCGAAAGCTTCCCTGAGCTGATGGATTAT
ACCTTTACTGCGGGGCTTGAAGATGAGCTTGATCATGTGGCACAAGGCGATCAAGATTGGAAAGCGGTGTTAGATGACTT
TTATGGGGACTTTAAGACTAAGCTTGAGCATGCCAAAGATAAAGACGGTATGCGCCCGAATGACCCCACCGATGTACCCG
ACGTGCATTGCGATTTATGTGGCCGACCAATGCAGCTGCGTAATGGCTCAACCGGTGTATTCTTAGGCTGTACTGGCTAC
AACTTGCCGCCCAAAGAGCGCTGTAAGGGCACCAAAAACCTAATGCCGGTAGAAGCCTTTGCCAACTTAGATGACAGCGA
AATCGATGAAAGTGCTGAAGTCAAAGAGTTGATGGAGAAAAAACGTTGTCCTAAGTGTAGCACAGCGATGGATGGCTATA
TTGTGGATGGTGGCCTCAAGCTGCATATCTGTGGTAACAACCCAGACTGTGATGGCTATTTATTAGAAGAAGGTATGTTC
GAGGTGCCAGGCTCTGATGCGCCGACCATTGACTGTAATAAATGTGATGGTCAGATGGAGCTTAAGACCGGCCGCTTTGG
TCCTTATTTTGCTTGTAATAAGTGTGATAATACCCGCAAAGTGCTCAAAAATGGTGAGCCTGCACCGCCGCGCATGGATC
CTATTGATATGCCAGAGCTTAAGTCGACCAAGCATGAGGATCACTTTATCTTACGAGAGGGGGCTGCCGGTCTATTCTTA
GCGGCCTCTAAGTTCCCTAAAGTGCGCGAGACGCGTCCCCCTAAGCTGTCTGAGCTACGCACCATTAAGGACAAGATGGA
TGATAAGTTCCAGTATTTGTTTGATGGTCCAGATCAAGATCCGGAGGGTAACCCTACCATTTTGCGCTGGTCACGTAAGC
AAAAAGAGCAGTACATTGGCTCTGAGAGCTTAAAAACAGGCAAGGCCACGCGTTGGGGCGTTTACTGGCGTAAAGGCGAG
TGGGTAGAGGAGTAG

Upstream 100 bases:

>100_bases
TTTAGGCCAAAGCCAGACATTTAAAGCAGATCTTTGGCTGAGCTCACCAGACTATAAAAAATATAATTTATAAGAAAGTT
TAATTAGGACTTTGATATCA

Downstream 100 bases:

>100_bases
CCTTTAGCCACAGCCATAAAAAATCCCAGTCAATGACTGGGATTTTTTATGTTAAATGATAATGATAGCGCTGTTAGATA
ACAACAAGGGGTAGCACCGC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 884; Mature: 883

Protein sequence:

>884_residues
MAKTTAKKTTSTPAASAKGKSLVIVESPAKAKTINKYLGDDFIVRSSIGHVRDLPTSSGVKKKATKTDEKLSKEDKAQQA
LVRRMGIDPEHGWKAVYEVLPNKTKVIKELKALAKDADKIYLATDLDREGEAIAWHLREVIGGDDSKYQRVVFNEITKSA
IQNAFKEPGKLDIDRVNAQQARRFLDRVVGFMVSPLLWQKIARGLSAGRVQSVATRLVVEREREIRAFIPEEYWQIHADT
TMKGSENANVAADTPIRLEAVKQAGKVLKLGNKAQTDAVLAVLKPATFVVKEREEKPTQSKPSAPFITSTLQQAASTRLG
FPVKKTMMLAQRLYEAGHITYMRTDSTFLSNDALASVRGFIEENYGSKYLPEKPNFYGSKQNAQEAHEAIRPTNANLKST
QLKGVDRDAIRLYELIWRQFVACQMLPAKYKSVNLLVEAGDVELKARGRTMTFDGFTKVMPPAKTDDTLLPDVKQGDELN
LIKLDPSQHFTKPPARYTEASLVKELEKKGIGRPSTYASIISTIQDRGYVRLENKRLYAEKMGDIVTDRLTESFPELMDY
TFTAGLEDELDHVAQGDQDWKAVLDDFYGDFKTKLEHAKDKDGMRPNDPTDVPDVHCDLCGRPMQLRNGSTGVFLGCTGY
NLPPKERCKGTKNLMPVEAFANLDDSEIDESAEVKELMEKKRCPKCSTAMDGYIVDGGLKLHICGNNPDCDGYLLEEGMF
EVPGSDAPTIDCNKCDGQMELKTGRFGPYFACNKCDNTRKVLKNGEPAPPRMDPIDMPELKSTKHEDHFILREGAAGLFL
AASKFPKVRETRPPKLSELRTIKDKMDDKFQYLFDGPDQDPEGNPTILRWSRKQKEQYIGSESLKTGKATRWGVYWRKGE
WVEE

Sequences:

>Translated_884_residues
MAKTTAKKTTSTPAASAKGKSLVIVESPAKAKTINKYLGDDFIVRSSIGHVRDLPTSSGVKKKATKTDEKLSKEDKAQQA
LVRRMGIDPEHGWKAVYEVLPNKTKVIKELKALAKDADKIYLATDLDREGEAIAWHLREVIGGDDSKYQRVVFNEITKSA
IQNAFKEPGKLDIDRVNAQQARRFLDRVVGFMVSPLLWQKIARGLSAGRVQSVATRLVVEREREIRAFIPEEYWQIHADT
TMKGSENANVAADTPIRLEAVKQAGKVLKLGNKAQTDAVLAVLKPATFVVKEREEKPTQSKPSAPFITSTLQQAASTRLG
FPVKKTMMLAQRLYEAGHITYMRTDSTFLSNDALASVRGFIEENYGSKYLPEKPNFYGSKQNAQEAHEAIRPTNANLKST
QLKGVDRDAIRLYELIWRQFVACQMLPAKYKSVNLLVEAGDVELKARGRTMTFDGFTKVMPPAKTDDTLLPDVKQGDELN
LIKLDPSQHFTKPPARYTEASLVKELEKKGIGRPSTYASIISTIQDRGYVRLENKRLYAEKMGDIVTDRLTESFPELMDY
TFTAGLEDELDHVAQGDQDWKAVLDDFYGDFKTKLEHAKDKDGMRPNDPTDVPDVHCDLCGRPMQLRNGSTGVFLGCTGY
NLPPKERCKGTKNLMPVEAFANLDDSEIDESAEVKELMEKKRCPKCSTAMDGYIVDGGLKLHICGNNPDCDGYLLEEGMF
EVPGSDAPTIDCNKCDGQMELKTGRFGPYFACNKCDNTRKVLKNGEPAPPRMDPIDMPELKSTKHEDHFILREGAAGLFL
AASKFPKVRETRPPKLSELRTIKDKMDDKFQYLFDGPDQDPEGNPTILRWSRKQKEQYIGSESLKTGKATRWGVYWRKGE
WVEE
>Mature_883_residues
AKTTAKKTTSTPAASAKGKSLVIVESPAKAKTINKYLGDDFIVRSSIGHVRDLPTSSGVKKKATKTDEKLSKEDKAQQAL
VRRMGIDPEHGWKAVYEVLPNKTKVIKELKALAKDADKIYLATDLDREGEAIAWHLREVIGGDDSKYQRVVFNEITKSAI
QNAFKEPGKLDIDRVNAQQARRFLDRVVGFMVSPLLWQKIARGLSAGRVQSVATRLVVEREREIRAFIPEEYWQIHADTT
MKGSENANVAADTPIRLEAVKQAGKVLKLGNKAQTDAVLAVLKPATFVVKEREEKPTQSKPSAPFITSTLQQAASTRLGF
PVKKTMMLAQRLYEAGHITYMRTDSTFLSNDALASVRGFIEENYGSKYLPEKPNFYGSKQNAQEAHEAIRPTNANLKSTQ
LKGVDRDAIRLYELIWRQFVACQMLPAKYKSVNLLVEAGDVELKARGRTMTFDGFTKVMPPAKTDDTLLPDVKQGDELNL
IKLDPSQHFTKPPARYTEASLVKELEKKGIGRPSTYASIISTIQDRGYVRLENKRLYAEKMGDIVTDRLTESFPELMDYT
FTAGLEDELDHVAQGDQDWKAVLDDFYGDFKTKLEHAKDKDGMRPNDPTDVPDVHCDLCGRPMQLRNGSTGVFLGCTGYN
LPPKERCKGTKNLMPVEAFANLDDSEIDESAEVKELMEKKRCPKCSTAMDGYIVDGGLKLHICGNNPDCDGYLLEEGMFE
VPGSDAPTIDCNKCDGQMELKTGRFGPYFACNKCDNTRKVLKNGEPAPPRMDPIDMPELKSTKHEDHFILREGAAGLFLA
ASKFPKVRETRPPKLSELRTIKDKMDDKFQYLFDGPDQDPEGNPTILRWSRKQKEQYIGSESLKTGKATRWGVYWRKGEW
VEE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=707, Percent_Identity=26.4497878359264, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI10835218, Length=581, Percent_Identity=25.473321858864, Blast_Score=133, Evalue=9e-31,
Organism=Escherichia coli, GI1787529, Length=875, Percent_Identity=62.2857142857143, Blast_Score=1118, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=649, Percent_Identity=22.9583975346687, Blast_Score=104, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=554, Percent_Identity=27.0758122743682, Blast_Score=142, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=534, Percent_Identity=25.2808988764045, Blast_Score=116, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=523, Percent_Identity=26.5774378585086, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24640096, Length=530, Percent_Identity=26.6037735849057, Blast_Score=126, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24585251, Length=642, Percent_Identity=24.6105919003115, Blast_Score=122, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 99120; Mature: 98989

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTTAKKTTSTPAASAKGKSLVIVESPAKAKTINKYLGDDFIVRSSIGHVRDLPTSSGV
CCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEHHCCCHHHCCCCCCCC
KKKATKTDEKLSKEDKAQQALVRRMGIDPEHGWKAVYEVLPNKTKVIKELKALAKDADKI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
YLATDLDREGEAIAWHLREVIGGDDSKYQRVVFNEITKSAIQNAFKEPGKLDIDRVNAQQ
EEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHH
ARRFLDRVVGFMVSPLLWQKIARGLSAGRVQSVATRLVVEREREIRAFIPEEYWQIHADT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCC
TMKGSENANVAADTPIRLEAVKQAGKVLKLGNKAQTDAVLAVLKPATFVVKEREEKPTQS
EECCCCCCCEECCCCHHHHHHHHCCHHEECCCCHHHHHHHHHHCCHHHEEHHHHCCCCCC
KPSAPFITSTLQQAASTRLGFPVKKTMMLAQRLYEAGHITYMRTDSTFLSNDALASVRGF
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHCCHHHHHHHHH
IEENYGSKYLPEKPNFYGSKQNAQEAHEAIRPTNANLKSTQLKGVDRDAIRLYELIWRQF
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHH
VACQMLPAKYKSVNLLVEAGDVELKARGRTMTFDGFTKVMPPAKTDDTLLPDVKQGDELN
HHHHHCCCCCCCEEEEEEECCEEEEECCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCEE
LIKLDPSQHFTKPPARYTEASLVKELEKKGIGRPSTYASIISTIQDRGYVRLENKRLYAE
EEEECCHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHH
KMGDIVTDRLTESFPELMDYTFTAGLEDELDHVAQGDQDWKAVLDDFYGDFKTKLEHAKD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
KDGMRPNDPTDVPDVHCDLCGRPMQLRNGSTGVFLGCTGYNLPPKERCKGTKNLMPVEAF
CCCCCCCCCCCCCCCHHHHCCCCCEECCCCCEEEEEECCCCCCCHHHCCCCCCCCCHHHH
ANLDDSEIDESAEVKELMEKKRCPKCSTAMDGYIVDGGLKLHICGNNPDCDGYLLEEGMF
CCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCCCCCEECCCCCE
EVPGSDAPTIDCNKCDGQMELKTGRFGPYFACNKCDNTRKVLKNGEPAPPRMDPIDMPEL
ECCCCCCCEEECCCCCCEEEEECCCCCCCEECCCCCHHHHHHHCCCCCCCCCCCCCCCCH
KSTKHEDHFILREGAAGLFLAASKFPKVRETRPPKLSELRTIKDKMDDKFQYLFDGPDQD
HCCCCCCCEEEECCCCCEEEEHHCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCCCCC
PEGNPTILRWSRKQKEQYIGSESLKTGKATRWGVYWRKGEWVEE
CCCCCCEEECCHHHHHHHCCCHHHCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
AKTTAKKTTSTPAASAKGKSLVIVESPAKAKTINKYLGDDFIVRSSIGHVRDLPTSSGV
CCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEHHCCCHHHCCCCCCCC
KKKATKTDEKLSKEDKAQQALVRRMGIDPEHGWKAVYEVLPNKTKVIKELKALAKDADKI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
YLATDLDREGEAIAWHLREVIGGDDSKYQRVVFNEITKSAIQNAFKEPGKLDIDRVNAQQ
EEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHH
ARRFLDRVVGFMVSPLLWQKIARGLSAGRVQSVATRLVVEREREIRAFIPEEYWQIHADT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCC
TMKGSENANVAADTPIRLEAVKQAGKVLKLGNKAQTDAVLAVLKPATFVVKEREEKPTQS
EECCCCCCCEECCCCHHHHHHHHCCHHEECCCCHHHHHHHHHHCCHHHEEHHHHCCCCCC
KPSAPFITSTLQQAASTRLGFPVKKTMMLAQRLYEAGHITYMRTDSTFLSNDALASVRGF
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHCCHHHHHHHHH
IEENYGSKYLPEKPNFYGSKQNAQEAHEAIRPTNANLKSTQLKGVDRDAIRLYELIWRQF
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHH
VACQMLPAKYKSVNLLVEAGDVELKARGRTMTFDGFTKVMPPAKTDDTLLPDVKQGDELN
HHHHHCCCCCCCEEEEEEECCEEEEECCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCEE
LIKLDPSQHFTKPPARYTEASLVKELEKKGIGRPSTYASIISTIQDRGYVRLENKRLYAE
EEEECCHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHH
KMGDIVTDRLTESFPELMDYTFTAGLEDELDHVAQGDQDWKAVLDDFYGDFKTKLEHAKD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
KDGMRPNDPTDVPDVHCDLCGRPMQLRNGSTGVFLGCTGYNLPPKERCKGTKNLMPVEAF
CCCCCCCCCCCCCCCHHHHCCCCCEECCCCCEEEEEECCCCCCCHHHCCCCCCCCCHHHH
ANLDDSEIDESAEVKELMEKKRCPKCSTAMDGYIVDGGLKLHICGNNPDCDGYLLEEGMF
CCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCCCCCEECCCCCE
EVPGSDAPTIDCNKCDGQMELKTGRFGPYFACNKCDNTRKVLKNGEPAPPRMDPIDMPEL
ECCCCCCCEEECCCCCCEEEEECCCCCCCEECCCCCHHHHHHHCCCCCCCCCCCCCCCCH
KSTKHEDHFILREGAAGLFLAASKFPKVRETRPPKLSELRTIKDKMDDKFQYLFDGPDQD
HCCCCCCCEEEECCCCCEEEEHHCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCCCCC
PEGNPTILRWSRKQKEQYIGSESLKTGKATRWGVYWRKGEWVEE
CCCCCCEEECCHHHHHHHCCCHHHCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]