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The map label for this gene is trpB [H]

Identifier: 162330297

GI number: 162330297

Start: 1120245

End: 1121444

Strand: Reverse

Name: trpB [H]

Synonym: Msm_1142

Alternate gene names: 162330297

Gene position: 1121444-1120245 (Counterclockwise)

Preceding gene: 148643203

Following gene: 148643201

Centisome position: 60.52

GC content: 36.75

Gene sequence:

>1200_bases
ATGGAGGCTAAAAACATGAATAATGGAAGATACGGGGAATATGGAGGACAATATATCTCTGAAACTTTAATGAATGAATT
GATTTACTTGGAAGAACAGTATTATCACTATATGAATGATTCTGAATTTGTAGAAGAATTAAATACACTTTTAAAAGAAT
ATGCTGGCAGACCTTCTTTATTATACTATGCAAAAAGAATGACTGAAGATTTGGGAGGAGCTAAGATTTATTTAAAGCGT
GAAGATTTAAATCACACTGGAGCTCATAAAATCAACAATGTGCTTGGTCAGGTTTTACTGGCTAAAAAAATGGGAAAAAC
AAGAGTAATAGCTGAAACTGGAGCAGGCCAACATGGGGTAGCTACAGCAACAGCAGCAGCACTTCTGGATATGGAATGTG
AAGTGTACATGGGTGAGTTAGATACCAAACGTCAGGCTTTAAATGTTTATAGGATGGAATTGCTTGGAGCAAAAGTTCAT
CCGGTAAAATCAGGAACAAAAACACTAAAGGATGCAGTTAATGATGCATTTAGAGATTGGATTGGGAGAGTTCATGATAC
AAATTATGTAATAGGTTCAACAATGGGGCCGCATCCTTTTCCAATGATGGTTAGAGACTTCCAAGCAGTAATAAGTGCTG
AAGCTCGTGAACAATTTTTGGAAAAAGAAGGAAAACTTCCGGATGCAGTTTTAGCATGTGTTGGTGGTGGAAGTAATGCA
ATGGGAGCATTTTACAATTTCATAGATGATGCGGATGTTAAATTAATTGGATGTGAAGCAGGAGGAAAAGGAGTAGATAC
TCCTTATAATGCAGCAGCTTTAACTAATGGGCAAATCGGAATATTTCACGGAATGAAATCAATATTTAATCAGGGAGAAT
ATGGGCAGATATCTGAAGTCTATTCTGTCTCAGCAGGTCTGGATTATCCTGGAGTAGGACCTGAACATGCATATTTAAGA
GATATTAAAAGAGCAGAGTATGTTCCTGTTACAGATGAAGAAGCAGTCAATGCTTTTGAATACTTGTCAAAAATGGAAGG
AATCATTCCGGCTATTGAAAGTGCTCATGCAGTAGCACATGCAATGAAAATAGCTCCAAAAATGGATAAAGACGAAATTA
TCATGGTTTGTCTTTCTGGAAGGGGAGACAAAGATGTAGCATCAATTGCAAAATATAGGGGAGTTGAGTTAAATGAGTAA

Upstream 100 bases:

>100_bases
CATCACTCAGGCTATTGGAGAATTTAAGCCATATGCAATTGATTTAAGCAGCGGTGTTGAAAGTGACGGATTTAAAGATG
AAAATAAAATAAAAAAAGTC

Downstream 100 bases:

>100_bases
AATAGCAAATGCATTTAAGGACGGTAAGGCATTTATCGGATTTTTAACTGCAGGAGATCCGACAATTGAAAAAACAGTAG
AATATATTTTGGCAATGGAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MEAKNMNNGRYGEYGGQYISETLMNELIYLEEQYYHYMNDSEFVEELNTLLKEYAGRPSLLYYAKRMTEDLGGAKIYLKR
EDLNHTGAHKINNVLGQVLLAKKMGKTRVIAETGAGQHGVATATAAALLDMECEVYMGELDTKRQALNVYRMELLGAKVH
PVKSGTKTLKDAVNDAFRDWIGRVHDTNYVIGSTMGPHPFPMMVRDFQAVISAEAREQFLEKEGKLPDAVLACVGGGSNA
MGAFYNFIDDADVKLIGCEAGGKGVDTPYNAAALTNGQIGIFHGMKSIFNQGEYGQISEVYSVSAGLDYPGVGPEHAYLR
DIKRAEYVPVTDEEAVNAFEYLSKMEGIIPAIESAHAVAHAMKIAPKMDKDEIIMVCLSGRGDKDVASIAKYRGVELNE

Sequences:

>Translated_399_residues
MEAKNMNNGRYGEYGGQYISETLMNELIYLEEQYYHYMNDSEFVEELNTLLKEYAGRPSLLYYAKRMTEDLGGAKIYLKR
EDLNHTGAHKINNVLGQVLLAKKMGKTRVIAETGAGQHGVATATAAALLDMECEVYMGELDTKRQALNVYRMELLGAKVH
PVKSGTKTLKDAVNDAFRDWIGRVHDTNYVIGSTMGPHPFPMMVRDFQAVISAEAREQFLEKEGKLPDAVLACVGGGSNA
MGAFYNFIDDADVKLIGCEAGGKGVDTPYNAAALTNGQIGIFHGMKSIFNQGEYGQISEVYSVSAGLDYPGVGPEHAYLR
DIKRAEYVPVTDEEAVNAFEYLSKMEGIIPAIESAHAVAHAMKIAPKMDKDEIIMVCLSGRGDKDVASIAKYRGVELNE
>Mature_399_residues
MEAKNMNNGRYGEYGGQYISETLMNELIYLEEQYYHYMNDSEFVEELNTLLKEYAGRPSLLYYAKRMTEDLGGAKIYLKR
EDLNHTGAHKINNVLGQVLLAKKMGKTRVIAETGAGQHGVATATAAALLDMECEVYMGELDTKRQALNVYRMELLGAKVH
PVKSGTKTLKDAVNDAFRDWIGRVHDTNYVIGSTMGPHPFPMMVRDFQAVISAEAREQFLEKEGKLPDAVLACVGGGSNA
MGAFYNFIDDADVKLIGCEAGGKGVDTPYNAAALTNGQIGIFHGMKSIFNQGEYGQISEVYSVSAGLDYPGVGPEHAYLR
DIKRAEYVPVTDEEAVNAFEYLSKMEGIIPAIESAHAVAHAMKIAPKMDKDEIIMVCLSGRGDKDVASIAKYRGVELNE

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=391, Percent_Identity=54.2199488491049, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321412, Length=383, Percent_Identity=55.3524804177546, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43888; Mature: 43888

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKNMNNGRYGEYGGQYISETLMNELIYLEEQYYHYMNDSEFVEELNTLLKEYAGRPSL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHH
LYYAKRMTEDLGGAKIYLKREDLNHTGAHKINNVLGQVLLAKKMGKTRVIAETGAGQHGV
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
ATATAAALLDMECEVYMGELDTKRQALNVYRMELLGAKVHPVKSGTKTLKDAVNDAFRDW
HHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IGRVHDTNYVIGSTMGPHPFPMMVRDFQAVISAEAREQFLEKEGKLPDAVLACVGGGSNA
HHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCH
MGAFYNFIDDADVKLIGCEAGGKGVDTPYNAAALTNGQIGIFHGMKSIFNQGEYGQISEV
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCHHHH
YSVSAGLDYPGVGPEHAYLRDIKRAEYVPVTDEEAVNAFEYLSKMEGIIPAIESAHAVAH
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMKIAPKMDKDEIIMVCLSGRGDKDVASIAKYRGVELNE
HHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEAKNMNNGRYGEYGGQYISETLMNELIYLEEQYYHYMNDSEFVEELNTLLKEYAGRPSL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHH
LYYAKRMTEDLGGAKIYLKREDLNHTGAHKINNVLGQVLLAKKMGKTRVIAETGAGQHGV
HHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
ATATAAALLDMECEVYMGELDTKRQALNVYRMELLGAKVHPVKSGTKTLKDAVNDAFRDW
HHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IGRVHDTNYVIGSTMGPHPFPMMVRDFQAVISAEAREQFLEKEGKLPDAVLACVGGGSNA
HHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCH
MGAFYNFIDDADVKLIGCEAGGKGVDTPYNAAALTNGQIGIFHGMKSIFNQGEYGQISEV
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCHHHH
YSVSAGLDYPGVGPEHAYLRDIKRAEYVPVTDEEAVNAFEYLSKMEGIIPAIESAHAVAH
HHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMKIAPKMDKDEIIMVCLSGRGDKDVASIAKYRGVELNE
HHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]