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The map label for this gene is trpA [H]

Identifier: 148643201

GI number: 148643201

Start: 1119479

End: 1120252

Strand: Reverse

Name: trpA [H]

Synonym: Msm_1141

Alternate gene names: 148643201

Gene position: 1120252-1119479 (Counterclockwise)

Preceding gene: 162330297

Following gene: 148643199

Centisome position: 60.45

GC content: 35.27

Gene sequence:

>774_bases
ATGAGTAAAATAGCAAATGCATTTAAGGACGGTAAGGCATTTATCGGATTTTTAACTGCAGGAGATCCGACAATTGAAAA
AACAGTAGAATATATTTTGGCAATGGAAGAAGCAGGTTGCGATTTAATAGAAATTGGAATTCCGTTTTCAGATCCTATGG
CTGAAGGAGTTGTAATTCAGGATGCAAATATAAGGGCATTAGGCCATAATACAACAACTGACGATGTATTTGAAATAGTC
AGGAAAGTTCGCCAAAAAACAGATGTTCCATTAGTATTTTTAACTTACATTAACCCTGTTTTCTTTTATGGTTATGAAAA
ATTCTTTAAAAAATGCGGTGAACTTGGAATCGATGGAATTATATCACCTGACCTGCCTTATGAAGAAAAAGGTGAAATCA
AAGACATTGCATTATCTAATGATGTTGATGTCATTTCTCTGATTGCTCCAACTTCAAAGGAAAGGATTCAAAAAATAGCC
AGTGATGCAACAGGTTTTATTTATGTTGTTTCTTCTTTGGGAGTTACTGGAATGCGCAGTGAAATAAAAACGGACCTGAA
ATCTATTTTAGATGATATTCGTGCAGTCAGTGACTTGCCATTAGCTGTTGGATTTGGTATTAACACACCTGAACAGGCTT
CTAACATTGGGAAAATAGCTGATGGAGTTATTGTGGGAAGTGCTATTGTAAAAATCATTGAGGAGCATGGTGAAAATGCA
GCAGAACCATTAAAAGAATATGTGTCAGCTATGAAAACAGCCTGCAATGAATAA

Upstream 100 bases:

>100_bases
GCTCCAAAAATGGATAAAGACGAAATTATCATGGTTTGTCTTTCTGGAAGGGGAGACAAAGATGTAGCATCAATTGCAAA
ATATAGGGGAGTTGAGTTAA

Downstream 100 bases:

>100_bases
AAAGGATAAACCTGAAATTACAAAAATAATATCGTATACATTTTATTGTATCAACAGCAGGTGTTACACACCACCATCAC
ATCTTTTTCATTATCATTAA

Product: tryptophan synthase, alpha subunit, TrpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSKIANAFKDGKAFIGFLTAGDPTIEKTVEYILAMEEAGCDLIEIGIPFSDPMAEGVVIQDANIRALGHNTTTDDVFEIV
RKVRQKTDVPLVFLTYINPVFFYGYEKFFKKCGELGIDGIISPDLPYEEKGEIKDIALSNDVDVISLIAPTSKERIQKIA
SDATGFIYVVSSLGVTGMRSEIKTDLKSILDDIRAVSDLPLAVGFGINTPEQASNIGKIADGVIVGSAIVKIIEEHGENA
AEPLKEYVSAMKTACNE

Sequences:

>Translated_257_residues
MSKIANAFKDGKAFIGFLTAGDPTIEKTVEYILAMEEAGCDLIEIGIPFSDPMAEGVVIQDANIRALGHNTTTDDVFEIV
RKVRQKTDVPLVFLTYINPVFFYGYEKFFKKCGELGIDGIISPDLPYEEKGEIKDIALSNDVDVISLIAPTSKERIQKIA
SDATGFIYVVSSLGVTGMRSEIKTDLKSILDDIRAVSDLPLAVGFGINTPEQASNIGKIADGVIVGSAIVKIIEEHGENA
AEPLKEYVSAMKTACNE
>Mature_256_residues
SKIANAFKDGKAFIGFLTAGDPTIEKTVEYILAMEEAGCDLIEIGIPFSDPMAEGVVIQDANIRALGHNTTTDDVFEIVR
KVRQKTDVPLVFLTYINPVFFYGYEKFFKKCGELGIDGIISPDLPYEEKGEIKDIALSNDVDVISLIAPTSKERIQKIAS
DATGFIYVVSSLGVTGMRSEIKTDLKSILDDIRAVSDLPLAVGFGINTPEQASNIGKIADGVIVGSAIVKIIEEHGENAA
EPLKEYVSAMKTACNE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=252, Percent_Identity=36.5079365079365, Blast_Score=159, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321412, Length=253, Percent_Identity=37.9446640316205, Blast_Score=178, Evalue=7e-46,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 27829; Mature: 27698

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIANAFKDGKAFIGFLTAGDPTIEKTVEYILAMEEAGCDLIEIGIPFSDPMAEGVVIQ
CCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEE
DANIRALGHNTTTDDVFEIVRKVRQKTDVPLVFLTYINPVFFYGYEKFFKKCGELGIDGI
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ISPDLPYEEKGEIKDIALSNDVDVISLIAPTSKERIQKIASDATGFIYVVSSLGVTGMRS
CCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHH
EIKTDLKSILDDIRAVSDLPLAVGFGINTPEQASNIGKIADGVIVGSAIVKIIEEHGENA
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AEPLKEYVSAMKTACNE
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKIANAFKDGKAFIGFLTAGDPTIEKTVEYILAMEEAGCDLIEIGIPFSDPMAEGVVIQ
CHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEE
DANIRALGHNTTTDDVFEIVRKVRQKTDVPLVFLTYINPVFFYGYEKFFKKCGELGIDGI
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ISPDLPYEEKGEIKDIALSNDVDVISLIAPTSKERIQKIASDATGFIYVVSSLGVTGMRS
CCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHH
EIKTDLKSILDDIRAVSDLPLAVGFGINTPEQASNIGKIADGVIVGSAIVKIIEEHGENA
HHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AEPLKEYVSAMKTACNE
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923 [H]