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The map label for this gene is murD [C]

Identifier: 148643251

GI number: 148643251

Start: 1188844

End: 1190226

Strand: Reverse

Name: murD [C]

Synonym: Msm_1191

Alternate gene names: 148643251

Gene position: 1190226-1188844 (Counterclockwise)

Preceding gene: 148643252

Following gene: 148643250

Centisome position: 64.23

GC content: 33.19

Gene sequence:

>1383_bases
ATGAATTATTTAGTTATTGGTGCAGGAAATGCTGGTAGGCCTGTTGCAAGGTTACTTAATAATCAAAATCATAAGGTTAT
TATAACTGATCCAAAACAGTTATCTGATTTTAAACAAGATGTGCAAAGTATTTTAAAACAAATGGAAAAAGAGGGAGTTA
TTTTAGATTTAGGTAATGATAATCCTTCTATTGATGGTATTGATAAAGTTTATAAGGCTCCAAGTTTGCCTGACAGTGCA
CCTATTGCAAAAAAAGTTGCTGAAGCTAATTTAGAGGTTATTACTAATGAAGATTTCTCAAAAATGGTAAATGATTTAAT
TCCTGTTGATATTATTGGAATCACGGGAACTATGGGTAAAACAACTACTACATTTATTACAACAAGCATTTTTAAACAGG
CAGGTTATAAAGTATGGTCCTGCTCTTCTTTAGTTAATAATTTAGTTAGTGAAGCTATTATTGATGGTATAGTTAAAGGT
AAAGCTCAAAATTGTGATATAGCTATTTTTGAACTTCCTCATGGAACTATAGGGCTTCTAAATGAATTGGATATTAAAAT
TGGTCTTTTAACCAATATTGCTGAAGACCATCTGTCTGAATTTGGAGGTTCTCTTGAGAAATATCAGCAACGTAAATTGA
TTTTGGAAAAAATGAGTGAAACATTTATTGCAAATAATTCCTGCAGGGATATTATAGCTCCGGTAAGGGGCAATGCTCTT
TATTATGCTTTAGATGATGATTGTGATTTTATTGGAACTGCCGGTGATGAAAAATTAACCCTCAAATATTCTAAAGGGGA
ATTTACAACACCGTTCCACATGGTCAGCTATTTCTTTGAAAACTCTGTAGGGGCCAGCAGTGTTGCTTTAACTTATGGTG
TTTCTCCGGAAGATATTACTGATGCATTATCTGAATTTAAAGGACTTCCTGCACATATGGAAGATGTTGGTGAGTATAAT
GGAAGAAAAGTTATTTTGGATTCTGCATTTTTATATGATGGAATGAAAATCACTCTTGAGTACTTTAAAGACGACAATGT
TGTACTGTTTCTAGATCATTTTGATACATTATCTAAAAGGGATAAGACAGAAGTCGGTCAGCTTGTTGGACAGTATGTGG
ATGTTATAATAGCTAGTGGATTTAATGAGGTTAACCAAACAGTTGAGATGGATGCTGCATATGAAGTTTTAGATGCTATT
GAAAATCCAAGTGCAATTAAAGTAGCTACTAGGGATATTACTGAAGCAGCCGCACTTACATTCAAATATTCCAAACCTGG
AGACATTATTCTGCATATGGGGCCACTTATAGCTTATGATAGAATTACCACTGTTGAAAAAATCATGAAAGGACTTGAAG
AGGGAAGTAAAAAATATGAATGA

Upstream 100 bases:

>100_bases
TTGTTTTTAATACTTAGTCGAAATGTAATCAACTTTTTAGGGTGTTGTCTTCAACTTTAAATTTAATAAAATCATATAAT
AATTATAGGTGAATAAATTT

Downstream 100 bases:

>100_bases
GTCTGATTTTCCGGAAGATACAACATTTGGAGTAATTGGAATTTGTGGAGCTAATTGTAATCTGGTAGCTAGAATTCTTA
AAGACCGTGGATTTGATGTT

Product: peptide ligase

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNYLVIGAGNAGRPVARLLNNQNHKVIITDPKQLSDFKQDVQSILKQMEKEGVILDLGNDNPSIDGIDKVYKAPSLPDSA
PIAKKVAEANLEVITNEDFSKMVNDLIPVDIIGITGTMGKTTTTFITTSIFKQAGYKVWSCSSLVNNLVSEAIIDGIVKG
KAQNCDIAIFELPHGTIGLLNELDIKIGLLTNIAEDHLSEFGGSLEKYQQRKLILEKMSETFIANNSCRDIIAPVRGNAL
YYALDDDCDFIGTAGDEKLTLKYSKGEFTTPFHMVSYFFENSVGASSVALTYGVSPEDITDALSEFKGLPAHMEDVGEYN
GRKVILDSAFLYDGMKITLEYFKDDNVVLFLDHFDTLSKRDKTEVGQLVGQYVDVIIASGFNEVNQTVEMDAAYEVLDAI
ENPSAIKVATRDITEAAALTFKYSKPGDIILHMGPLIAYDRITTVEKIMKGLEEGSKKYE

Sequences:

>Translated_460_residues
MNYLVIGAGNAGRPVARLLNNQNHKVIITDPKQLSDFKQDVQSILKQMEKEGVILDLGNDNPSIDGIDKVYKAPSLPDSA
PIAKKVAEANLEVITNEDFSKMVNDLIPVDIIGITGTMGKTTTTFITTSIFKQAGYKVWSCSSLVNNLVSEAIIDGIVKG
KAQNCDIAIFELPHGTIGLLNELDIKIGLLTNIAEDHLSEFGGSLEKYQQRKLILEKMSETFIANNSCRDIIAPVRGNAL
YYALDDDCDFIGTAGDEKLTLKYSKGEFTTPFHMVSYFFENSVGASSVALTYGVSPEDITDALSEFKGLPAHMEDVGEYN
GRKVILDSAFLYDGMKITLEYFKDDNVVLFLDHFDTLSKRDKTEVGQLVGQYVDVIIASGFNEVNQTVEMDAAYEVLDAI
ENPSAIKVATRDITEAAALTFKYSKPGDIILHMGPLIAYDRITTVEKIMKGLEEGSKKYE
>Mature_460_residues
MNYLVIGAGNAGRPVARLLNNQNHKVIITDPKQLSDFKQDVQSILKQMEKEGVILDLGNDNPSIDGIDKVYKAPSLPDSA
PIAKKVAEANLEVITNEDFSKMVNDLIPVDIIGITGTMGKTTTTFITTSIFKQAGYKVWSCSSLVNNLVSEAIIDGIVKG
KAQNCDIAIFELPHGTIGLLNELDIKIGLLTNIAEDHLSEFGGSLEKYQQRKLILEKMSETFIANNSCRDIIAPVRGNAL
YYALDDDCDFIGTAGDEKLTLKYSKGEFTTPFHMVSYFFENSVGASSVALTYGVSPEDITDALSEFKGLPAHMEDVGEYN
GRKVILDSAFLYDGMKITLEYFKDDNVVLFLDHFDTLSKRDKTEVGQLVGQYVDVIIASGFNEVNQTVEMDAAYEVLDAI
ENPSAIKVATRDITEAAALTFKYSKPGDIILHMGPLIAYDRITTVEKIMKGLEEGSKKYE

Specific function: Cell Wall Formation. Catalyzes The Addition Of Glutamate To The Nucleotide Precursor Udp-N-Acetylmuramoyl-L- Alanine (Uma). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013221
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: 6.3.2.9

Molecular weight: Translated: 50595; Mature: 50595

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLVIGAGNAGRPVARLLNNQNHKVIITDPKQLSDFKQDVQSILKQMEKEGVILDLGND
CCEEEEECCCCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEECCC
NPSIDGIDKVYKAPSLPDSAPIAKKVAEANLEVITNEDFSKMVNDLIPVDIIGITGTMGK
CCCCCCHHHHHCCCCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHCCCEEEEEECCCCCC
TTTTFITTSIFKQAGYKVWSCSSLVNNLVSEAIIDGIVKGKAQNCDIAIFELPHGTIGLL
CHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECH
NELDIKIGLLTNIAEDHLSEFGGSLEKYQQRKLILEKMSETFIANNSCRDIIAPVRGNAL
HHCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEE
YYALDDDCDFIGTAGDEKLTLKYSKGEFTTPFHMVSYFFENSVGASSVALTYGVSPEDIT
EEEECCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
DALSEFKGLPAHMEDVGEYNGRKVILDSAFLYDGMKITLEYFKDDNVVLFLDHFDTLSKR
HHHHHHCCCCCHHHHHHCCCCCEEEEECHHHHCCEEEEEEEECCCCEEEEEECCHHHHHC
DKTEVGQLVGQYVDVIIASGFNEVNQTVEMDAAYEVLDAIENPSAIKVATRDITEAAALT
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHEE
FKYSKPGDIILHMGPLIAYDRITTVEKIMKGLEEGSKKYE
EEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYLVIGAGNAGRPVARLLNNQNHKVIITDPKQLSDFKQDVQSILKQMEKEGVILDLGND
CCEEEEECCCCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEEECCC
NPSIDGIDKVYKAPSLPDSAPIAKKVAEANLEVITNEDFSKMVNDLIPVDIIGITGTMGK
CCCCCCHHHHHCCCCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHCCCEEEEEECCCCCC
TTTTFITTSIFKQAGYKVWSCSSLVNNLVSEAIIDGIVKGKAQNCDIAIFELPHGTIGLL
CHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECH
NELDIKIGLLTNIAEDHLSEFGGSLEKYQQRKLILEKMSETFIANNSCRDIIAPVRGNAL
HHCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEE
YYALDDDCDFIGTAGDEKLTLKYSKGEFTTPFHMVSYFFENSVGASSVALTYGVSPEDIT
EEEECCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
DALSEFKGLPAHMEDVGEYNGRKVILDSAFLYDGMKITLEYFKDDNVVLFLDHFDTLSKR
HHHHHHCCCCCHHHHHHCCCCCEEEEECHHHHCCEEEEEEEECCCCEEEEEECCHHHHHC
DKTEVGQLVGQYVDVIIASGFNEVNQTVEMDAAYEVLDAIENPSAIKVATRDITEAAALT
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHEE
FKYSKPGDIILHMGPLIAYDRITTVEKIMKGLEEGSKKYE
EEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Potassium phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {MgATP2-}} 0.01 {dihydrouridine} 0.28 {D-Glu}} 0.13 {D-Glu}} 0.055 {D-Glu}} 0.03 {UDP-N-acetylmuramoyl-L-Ala}} 0.0075 {UDP-N-acetylmuramoyl-L-Ala}} 0.0055 {UDP-N-acetylmuramoyl-L-Ala}} 0.2 {1-phospho-N-acetylmuramoyl-L-Ala}} [C

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanine; glutamate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: Pentasodium salt; 1-Phospho-N-acetyl muramoyl -L-Ala-D-Glu; 2, 4, 6-Trinitrobenzenesulfonic acid; D-2-Aminoadipic acid; D-Asp; D-erythro-3-Methyl glutamic acid; D-erythro-4-Methyl glutamic acid; D-Gln; D-Pyroglutamic acid; DL-2-Amino-4-phosphonobutyric ac

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]