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The map label for this gene is pheA [H]

Identifier: 148643112

GI number: 148643112

Start: 1013135

End: 1013941

Strand: Reverse

Name: pheA [H]

Synonym: Msm_1052

Alternate gene names: 148643112

Gene position: 1013941-1013135 (Counterclockwise)

Preceding gene: 148643113

Following gene: 148643111

Centisome position: 54.71

GC content: 31.97

Gene sequence:

>807_bases
ATGTGTAATAATATTTCATTTTTAGGTCCGAAAGGAACTTTTACTCATGAAGCAGCTTATATACTTGAGGGAAATTTAAT
TCCATATTGCACGATTCCTGCAGTGATGGAAAGTGTTTCAAATGGGGAGTGTCAATTTGGAGTAGTGCCAATTGAAAACT
CTATTGAAGGTCCGGTAGGACTTACTCTAGATTCTTTAGCTCATAAATTTGATTTAAAAATATTTAAGGAAATGGTAATT
CCAATTAATCAGAATTTAATTGTCAATCCTAAAACAGTTATGAATGACATAACTGATGTATATTCTCATTCTCAGGCAAT
AGCTCAATGTCAGGAGTTTATTAGGGATAATGAAATACAGCCCCATTATGCTGTAAGTACTGCCAGTGCAGCTAAAAGTA
TTTTAGGCGATAAAACAAAGGCAGCTATTGGAAATTCTAAAGCTGCTGAGATTTATGGCTTGGAAATCCTCAAACCTAAT
ATTCAGGATTTGGATAATAACGAAACACGTTTTGTAGTTTTATCGAAAAATGATCATGAGCCTACTGGCAGGGATAAGAC
TTCAATAATATTCTCTATTTATGAAGATAAACCTGGTATGCTTTATAAAATATTAGGTGTTTTTGAAAAAGAAAGTATTA
ACTTAACAAAAATTGAATCAAGACCTTCTAAAAAAGGTTTGGGCAAATATTTGTTTTTTGTTGATTTTTATGGTCATAGA
AAAGATAAAACTGTTCAGAATATTCTAAATGAACTGGATGGGCTTACTTACTTTTTAAAGGTTTTAGGTTCTTACCCGGA
GTTTTAG

Upstream 100 bases:

>100_bases
AGTCTTAATTAAATTTTAATAGCTAATATTTTAGCTATTTCTTCTTTTTTATTTTTTACAATAAACTTTTTAACTGTCAA
GCAATATAACACAAAGTATT

Downstream 100 bases:

>100_bases
AAAAACTTTAAATTATTGGTTTAATAAACTAATATTATGATAAGATTCAGGGATGGTATACATGCCAAATGATAAGGAGA
AACTTCTAAGAGTTATGCGT

Product: prephenate dehydratase, PheA

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MCNNISFLGPKGTFTHEAAYILEGNLIPYCTIPAVMESVSNGECQFGVVPIENSIEGPVGLTLDSLAHKFDLKIFKEMVI
PINQNLIVNPKTVMNDITDVYSHSQAIAQCQEFIRDNEIQPHYAVSTASAAKSILGDKTKAAIGNSKAAEIYGLEILKPN
IQDLDNNETRFVVLSKNDHEPTGRDKTSIIFSIYEDKPGMLYKILGVFEKESINLTKIESRPSKKGLGKYLFFVDFYGHR
KDKTVQNILNELDGLTYFLKVLGSYPEF

Sequences:

>Translated_268_residues
MCNNISFLGPKGTFTHEAAYILEGNLIPYCTIPAVMESVSNGECQFGVVPIENSIEGPVGLTLDSLAHKFDLKIFKEMVI
PINQNLIVNPKTVMNDITDVYSHSQAIAQCQEFIRDNEIQPHYAVSTASAAKSILGDKTKAAIGNSKAAEIYGLEILKPN
IQDLDNNETRFVVLSKNDHEPTGRDKTSIIFSIYEDKPGMLYKILGVFEKESINLTKIESRPSKKGLGKYLFFVDFYGHR
KDKTVQNILNELDGLTYFLKVLGSYPEF
>Mature_268_residues
MCNNISFLGPKGTFTHEAAYILEGNLIPYCTIPAVMESVSNGECQFGVVPIENSIEGPVGLTLDSLAHKFDLKIFKEMVI
PINQNLIVNPKTVMNDITDVYSHSQAIAQCQEFIRDNEIQPHYAVSTASAAKSILGDKTKAAIGNSKAAEIYGLEILKPN
IQDLDNNETRFVVLSKNDHEPTGRDKTSIIFSIYEDKPGMLYKILGVFEKESINLTKIESRPSKKGLGKYLFFVDFYGHR
KDKTVQNILNELDGLTYFLKVLGSYPEF

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=280, Percent_Identity=31.0714285714286, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324013, Length=291, Percent_Identity=26.4604810996564, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 29908; Mature: 29908

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNNISFLGPKGTFTHEAAYILEGNLIPYCTIPAVMESVSNGECQFGVVPIENSIEGPVG
CCCCCCCCCCCCCCCCCEEEEEECCCCCEECHHHHHHHCCCCCEEEEEEECCCCCCCCCC
LTLDSLAHKFDLKIFKEMVIPINQNLIVNPKTVMNDITDVYSHSQAIAQCQEFIRDNEIQ
CCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PHYAVSTASAAKSILGDKTKAAIGNSKAAEIYGLEILKPNIQDLDNNETRFVVLSKNDHE
CCEEEHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEECCCHHCCCCCCEEEEEEECCCCC
PTGRDKTSIIFSIYEDKPGMLYKILGVFEKESINLTKIESRPSKKGLGKYLFFVDFYGHR
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEEECCCC
KDKTVQNILNELDGLTYFLKVLGSYPEF
CCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCNNISFLGPKGTFTHEAAYILEGNLIPYCTIPAVMESVSNGECQFGVVPIENSIEGPVG
CCCCCCCCCCCCCCCCCEEEEEECCCCCEECHHHHHHHCCCCCEEEEEEECCCCCCCCCC
LTLDSLAHKFDLKIFKEMVIPINQNLIVNPKTVMNDITDVYSHSQAIAQCQEFIRDNEIQ
CCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PHYAVSTASAAKSILGDKTKAAIGNSKAAEIYGLEILKPNIQDLDNNETRFVVLSKNDHE
CCEEEHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEECCCHHCCCCCCEEEEEEECCCCC
PTGRDKTSIIFSIYEDKPGMLYKILGVFEKESINLTKIESRPSKKGLGKYLFFVDFYGHR
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEEECCCC
KDKTVQNILNELDGLTYFLKVLGSYPEF
CCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]