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The map label for this gene is ppsA [H]

Identifier: 148643048

GI number: 148643048

Start: 947690

End: 949972

Strand: Reverse

Name: ppsA [H]

Synonym: Msm_0988

Alternate gene names: 148643048

Gene position: 949972-947690 (Counterclockwise)

Preceding gene: 148643049

Following gene: 148643047

Centisome position: 51.26

GC content: 36.71

Gene sequence:

>2283_bases
ATGTATGTTGTAAAATTTGAGGATTTAGGCAAATCTGATATTGGAATTGCAGGGGGAAAAGGAGCTAACTTAGGAGAATT
GACTCAAGCAGGCATTCCTGTTCCGCCTGGTTTTGTAGTAACTGCCGAAACTTACGGAAAATTCATGGAAGATGCTGGAA
TTAATGGTAAGGTATTGGATATTCTTGCTAAAACAGATATCAACAATACTAAAGAACTTCAAGCTGCTGCTGAAGAAATT
AAATCCATCATTATTGGAACTCCAGTTCCAGAAGACATGTCCACTTTAATTATTGAAGCTTATAATCAACTTTGCCAAAG
AGTTGATGAAGATGATACTGATGTAGCTATTCGTTCTTCAGCTACTGCAGAAGATTTACCTGATGCTTCATTTGCAGGTC
AGCAAGACACTTTTTTACATGTTTCAGGTAGTGAAGATGTAATTGATTACATAAGAAAATGTTGGGCATCTTTATTTGAA
GCAAGAGCTATTTTTTACAGGGAAGAAAACGGTTTTGATCACTCAAAAGTATACATTGCTGTTGTTGTTCAAAAAATGGC
TATTGCAGATAAGGCAGGTGTAATGTTTACTGTAAACCCATCTACCGGTGAAGAAATAGCTTTAATTGAAGGATCATGGG
GTCTTGGTGAAGCTGTTGTGTCAGGTGACGTGACTCCTGATAATTATGTTGTAGACAAAAGCAATGATGAAGTTGTAAGT
GTCACTATAAGTGATAAAAAATTAATGTATGTAAAAGACGAAGACGGAACCAGTGTAAGAGTAGATGTTCCTGAAGATAA
AAGAAACGAAAGAGTTTTATCTGATGAAGAGCTTGTTGAGTTAACTGAAATGGGTAAAAGGATTCAAGCTCATTATGGCG
AACCAATGGATACTGAATGGGCTTTTGAAAGAGGCAATTTATTCTTATTACAGGCAAGGCCAATTACTACATTAGGTGAT
GACAATGGCGTAGCTGGTGAAGACTCTTCTATCAGTGATAAAAATGTCATTGTTAAAGGTCTTGGAGCAAGTCCAGGTAC
TGCTTCAGGTAAAGTCAAAATCATTTTAGACATTGATGAATTGGATAAAATTAAAGATGGAGACATTATGGTTACAACTA
TGACTACTCCTGATATGGTTCCAGCTATGAGAAGAGCTAGCGGAATCATTACTGATGAAGGGGGAGTAACTTGTCATGCA
TCTATCATTTCCCGTGAACTTGGAATTCCTTGTGTTGTAGGAACTGGTTCAGCTACAACTACATTAAAAGAGAATGTTGG
AGTTACTTTAGATGGTAAAAAAGGTTTGGTTTATGAAGGAATTCATGAAAACAAAGAGGAAGCAGTTCAAACAGCTACAT
CTAATGTTGAAGCAGCTCCAATCATAACTGTTACTGAAGTAAAAGCTAATGTAAGTATGCCTGAAGCAGCCCAAAGAGCA
GCAGCTACTGGTGCAGATGGAGTTGGATTGCTCAGAACTGAACATTTGATGTTGACTGCAGGTATTCACCCTGGAAAATT
CATTGCTGATGGAAGGGAAGATGAATTAATCGACACCATTGCAGAAAATGTAATGATTGTGGCAGACGCATTTTATCCAA
AACCTGTATGGTACAGAACTTTAGATGCGCCAACTGACGAATTCATCACTTTGGAAGGTGGAGAAAACGAACCTGAAGAA
CACAATCCAATGTTAGGTTGGAGAGGAATCAGAAGGGAACTTGACCAACCTGAAATTCTTAAATGTGAATTCAAAGCTAT
TAAAAAACTTTATGATAAAGGTTACACCAACTTAGGAATCATGATTCCATTATCACAAAGTCCTGCTGAACTTAAACAAG
CAAAAGAACTTTGTGCTAGTGTAGGTTTAATCCCTCATAAAGACATTGACTTTGGTATGATGGTAGAAATACCTGCAGCT
GCTTTAACTATTGAAGATTATCTTGAAATTGGTGTTGATTTCGTAAGTTTAGGAACTAATGATTTAACACAATATACTCT
TGCAGTAGACAGAAACAACGAATTTGTTGCAAAACATTATACTGAAGAACACCCTGCTGTAATGAAATTAATTGAAAGAA
CAATTAAAAAATGTGTTGAAGCTGGAGTTACTTGCAGTATTTGTGGTCAAGCAGGTAGTGTACCTCACATTGTTGAAAAA
CTTGTAGGATTTGGAATTACCAGTGTATCCTCAAATGCTGATGCTGTAGCTGAAGTTAGAAAAACTGTAGCTAGAGCTGA
GAAAAAAATTATTCTTGATGCTGCTCGCAAATCTCAAGAATAA

Upstream 100 bases:

>100_bases
TTTATACATTAAATATAATATTGGCGATGTTTTTTATTTATTGAGAACATGCTGTCAAGTATTAATATTAATTCATTTTA
AACCAAAGAGGTTATAATTC

Downstream 100 bases:

>100_bases
ATTTTATTTTCTTTTTTATTTTTTTTAAATATCACCTTATAAGGTTGTACTATGGAAGATAAACCAATATCTAAAAAAGA
CATACTTAAAGAATTAAATG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MYVVKFEDLGKSDIGIAGGKGANLGELTQAGIPVPPGFVVTAETYGKFMEDAGINGKVLDILAKTDINNTKELQAAAEEI
KSIIIGTPVPEDMSTLIIEAYNQLCQRVDEDDTDVAIRSSATAEDLPDASFAGQQDTFLHVSGSEDVIDYIRKCWASLFE
ARAIFYREENGFDHSKVYIAVVVQKMAIADKAGVMFTVNPSTGEEIALIEGSWGLGEAVVSGDVTPDNYVVDKSNDEVVS
VTISDKKLMYVKDEDGTSVRVDVPEDKRNERVLSDEELVELTEMGKRIQAHYGEPMDTEWAFERGNLFLLQARPITTLGD
DNGVAGEDSSISDKNVIVKGLGASPGTASGKVKIILDIDELDKIKDGDIMVTTMTTPDMVPAMRRASGIITDEGGVTCHA
SIISRELGIPCVVGTGSATTTLKENVGVTLDGKKGLVYEGIHENKEEAVQTATSNVEAAPIITVTEVKANVSMPEAAQRA
AATGADGVGLLRTEHLMLTAGIHPGKFIADGREDELIDTIAENVMIVADAFYPKPVWYRTLDAPTDEFITLEGGENEPEE
HNPMLGWRGIRRELDQPEILKCEFKAIKKLYDKGYTNLGIMIPLSQSPAELKQAKELCASVGLIPHKDIDFGMMVEIPAA
ALTIEDYLEIGVDFVSLGTNDLTQYTLAVDRNNEFVAKHYTEEHPAVMKLIERTIKKCVEAGVTCSICGQAGSVPHIVEK
LVGFGITSVSSNADAVAEVRKTVARAEKKIILDAARKSQE

Sequences:

>Translated_760_residues
MYVVKFEDLGKSDIGIAGGKGANLGELTQAGIPVPPGFVVTAETYGKFMEDAGINGKVLDILAKTDINNTKELQAAAEEI
KSIIIGTPVPEDMSTLIIEAYNQLCQRVDEDDTDVAIRSSATAEDLPDASFAGQQDTFLHVSGSEDVIDYIRKCWASLFE
ARAIFYREENGFDHSKVYIAVVVQKMAIADKAGVMFTVNPSTGEEIALIEGSWGLGEAVVSGDVTPDNYVVDKSNDEVVS
VTISDKKLMYVKDEDGTSVRVDVPEDKRNERVLSDEELVELTEMGKRIQAHYGEPMDTEWAFERGNLFLLQARPITTLGD
DNGVAGEDSSISDKNVIVKGLGASPGTASGKVKIILDIDELDKIKDGDIMVTTMTTPDMVPAMRRASGIITDEGGVTCHA
SIISRELGIPCVVGTGSATTTLKENVGVTLDGKKGLVYEGIHENKEEAVQTATSNVEAAPIITVTEVKANVSMPEAAQRA
AATGADGVGLLRTEHLMLTAGIHPGKFIADGREDELIDTIAENVMIVADAFYPKPVWYRTLDAPTDEFITLEGGENEPEE
HNPMLGWRGIRRELDQPEILKCEFKAIKKLYDKGYTNLGIMIPLSQSPAELKQAKELCASVGLIPHKDIDFGMMVEIPAA
ALTIEDYLEIGVDFVSLGTNDLTQYTLAVDRNNEFVAKHYTEEHPAVMKLIERTIKKCVEAGVTCSICGQAGSVPHIVEK
LVGFGITSVSSNADAVAEVRKTVARAEKKIILDAARKSQE
>Mature_760_residues
MYVVKFEDLGKSDIGIAGGKGANLGELTQAGIPVPPGFVVTAETYGKFMEDAGINGKVLDILAKTDINNTKELQAAAEEI
KSIIIGTPVPEDMSTLIIEAYNQLCQRVDEDDTDVAIRSSATAEDLPDASFAGQQDTFLHVSGSEDVIDYIRKCWASLFE
ARAIFYREENGFDHSKVYIAVVVQKMAIADKAGVMFTVNPSTGEEIALIEGSWGLGEAVVSGDVTPDNYVVDKSNDEVVS
VTISDKKLMYVKDEDGTSVRVDVPEDKRNERVLSDEELVELTEMGKRIQAHYGEPMDTEWAFERGNLFLLQARPITTLGD
DNGVAGEDSSISDKNVIVKGLGASPGTASGKVKIILDIDELDKIKDGDIMVTTMTTPDMVPAMRRASGIITDEGGVTCHA
SIISRELGIPCVVGTGSATTTLKENVGVTLDGKKGLVYEGIHENKEEAVQTATSNVEAAPIITVTEVKANVSMPEAAQRA
AATGADGVGLLRTEHLMLTAGIHPGKFIADGREDELIDTIAENVMIVADAFYPKPVWYRTLDAPTDEFITLEGGENEPEE
HNPMLGWRGIRRELDQPEILKCEFKAIKKLYDKGYTNLGIMIPLSQSPAELKQAKELCASVGLIPHKDIDFGMMVEIPAA
ALTIEDYLEIGVDFVSLGTNDLTQYTLAVDRNNEFVAKHYTEEHPAVMKLIERTIKKCVEAGVTCSICGQAGSVPHIVEK
LVGFGITSVSSNADAVAEVRKTVARAEKKIILDAARKSQE

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=795, Percent_Identity=37.7358490566038, Blast_Score=461, Evalue=1e-130,
Organism=Escherichia coli, GI1788756, Length=411, Percent_Identity=34.0632603406326, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789193, Length=400, Percent_Identity=30.75, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI48994992, Length=348, Percent_Identity=29.5977011494253, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1788726, Length=416, Percent_Identity=25, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17564524, Length=324, Percent_Identity=31.1728395061728, Blast_Score=110, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 82153; Mature: 82153

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVVKFEDLGKSDIGIAGGKGANLGELTQAGIPVPPGFVVTAETYGKFMEDAGINGKVLD
CEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEE
ILAKTDINNTKELQAAAEEIKSIIIGTPVPEDMSTLIIEAYNQLCQRVDEDDTDVAIRSS
EEEECCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
ATAEDLPDASFAGQQDTFLHVSGSEDVIDYIRKCWASLFEARAIFYREENGFDHSKVYIA
CCHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHEEHH
VVVQKMAIADKAGVMFTVNPSTGEEIALIEGSWGLGEAVVSGDVTPDNYVVDKSNDEVVS
HHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCHHEECCCCCCCEEEECCCCCEEE
VTISDKKLMYVKDEDGTSVRVDVPEDKRNERVLSDEELVELTEMGKRIQAHYGEPMDTEW
EEECCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
AFERGNLFLLQARPITTLGDDNGVAGEDSSISDKNVIVKGLGASPGTASGKVKIILDIDE
EEECCCEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECHH
LDKIKDGDIMVTTMTTPDMVPAMRRASGIITDEGGVTCHASIISRELGIPCVVGTGSATT
HCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCCCCEEEHHHHHHHCCCEEEEECCCCCE
TLKENVGVTLDGKKGLVYEGIHENKEEAVQTATSNVEAAPIITVTEVKANVSMPEAAQRA
EECCCCCEEEECCCCCEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHH
AATGADGVGLLRTEHLMLTAGIHPGKFIADGREDELIDTIAENVMIVADAFYPKPVWYRT
HCCCCCCCEEEEECEEEEEECCCCCCEECCCCCHHHHHHHHCCEEEEEECCCCCCCEEEE
LDAPTDEFITLEGGENEPEEHNPMLGWRGIRRELDQPEILKCEFKAIKKLYDKGYTNLGI
CCCCCCCEEEEECCCCCCHHCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCEEE
MIPLSQSPAELKQAKELCASVGLIPHKDIDFGMMVEIPAAALTIEDYLEIGVDFVSLGTN
EEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCEEEECCCC
DLTQYTLAVDRNNEFVAKHYTEEHPAVMKLIERTIKKCVEAGVTCSICGQAGSVPHIVEK
CCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHH
LVGFGITSVSSNADAVAEVRKTVARAEKKIILDAARKSQE
HHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYVVKFEDLGKSDIGIAGGKGANLGELTQAGIPVPPGFVVTAETYGKFMEDAGINGKVLD
CEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEE
ILAKTDINNTKELQAAAEEIKSIIIGTPVPEDMSTLIIEAYNQLCQRVDEDDTDVAIRSS
EEEECCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
ATAEDLPDASFAGQQDTFLHVSGSEDVIDYIRKCWASLFEARAIFYREENGFDHSKVYIA
CCHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHEEHH
VVVQKMAIADKAGVMFTVNPSTGEEIALIEGSWGLGEAVVSGDVTPDNYVVDKSNDEVVS
HHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCCHHEECCCCCCCEEEECCCCCEEE
VTISDKKLMYVKDEDGTSVRVDVPEDKRNERVLSDEELVELTEMGKRIQAHYGEPMDTEW
EEECCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
AFERGNLFLLQARPITTLGDDNGVAGEDSSISDKNVIVKGLGASPGTASGKVKIILDIDE
EEECCCEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECHH
LDKIKDGDIMVTTMTTPDMVPAMRRASGIITDEGGVTCHASIISRELGIPCVVGTGSATT
HCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCCCCEEEHHHHHHHCCCEEEEECCCCCE
TLKENVGVTLDGKKGLVYEGIHENKEEAVQTATSNVEAAPIITVTEVKANVSMPEAAQRA
EECCCCCEEEECCCCCEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHH
AATGADGVGLLRTEHLMLTAGIHPGKFIADGREDELIDTIAENVMIVADAFYPKPVWYRT
HCCCCCCCEEEEECEEEEEECCCCCCEECCCCCHHHHHHHHCCEEEEEECCCCCCCEEEE
LDAPTDEFITLEGGENEPEEHNPMLGWRGIRRELDQPEILKCEFKAIKKLYDKGYTNLGI
CCCCCCCEEEEECCCCCCHHCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCEEE
MIPLSQSPAELKQAKELCASVGLIPHKDIDFGMMVEIPAAALTIEDYLEIGVDFVSLGTN
EEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCEEEECCCC
DLTQYTLAVDRNNEFVAKHYTEEHPAVMKLIERTIKKCVEAGVTCSICGQAGSVPHIVEK
CCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHH
LVGFGITSVSSNADAVAEVRKTVARAEKKIILDAARKSQE
HHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]