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The map label for this gene is rpoB2 [H]

Identifier: 148642971

GI number: 148642971

Start: 876156

End: 877700

Strand: Reverse

Name: rpoB2 [H]

Synonym: Msm_0911

Alternate gene names: 148642971

Gene position: 877700-876156 (Counterclockwise)

Preceding gene: 148642972

Following gene: 148642970

Centisome position: 47.36

GC content: 33.33

Gene sequence:

>1545_bases
ATGACAGAGAATAACTGGAAATTAGTTGATGCATTTTTTGATAAATATGATTTAGTTGACCATCATATTAAATCATACAA
CGATTTTATAAATAGTGGGATTCAAAATATCATAGATATTACTGAACCTATTACTTTAGAAAACGGTAAATATACTTTAA
AAACCGGTAAACTCTTTATTGAAAAGCCATTCACTAAAGAAGCAGACGGTTCCAAAAGTATGATTTATCCTGCTGAAGCA
AGACTTAGGAATTTAAATTATTCTGCACACATGTATCTTGAGATGGCATTAAATGAAGAAGGAGAAGACAATCCTTTAGA
AAAAGTATATATTGGTGAATTGCCAGTAATGCTTAAATCAGATATTTGTCATCTTCATGGTCTTAGTGATGAAGAATTAA
TTGAACAGGGTGAAGACCCTCAAGATTTAGGTGGTTACTTCATTGTAAACGGTTCTGAAAGAGCTGTTGTAACAATGGAA
GAAATTGCACCAAATAAAATTATTCTTGAGCGTATTGGTGATGTTGAAGACAGAAGAGCTAAGGCTATTGTAACTTCTAT
TAAAAGTGGTTTCAGAGCAAGAATTTCTCTTGAATATAAAAAACCACGTAAAAGCGGAGTATTTTTAAGAATTTCTTTCC
CTTATGTTCCAGGGGAAATTCCACTTGTTATTTTATTAAGAGCTCTTGGTTTGGCTACAGGTCAGGAAATTATTACTGCA
ATTTCCAATGATCCAAATTTCCAAATGATTATTGCAGATGATATTCAGGTATCCCTTTCTCAATTGAAATTAGATCAAAA
AGAAATGGACGGCATGGATAAAGAAGAAAGAAGACAATATTTACAATTGGCTGCTATTAAATACATAGGTAATCGTGTAG
CTAAAGGTATGACTGAAGAATACCGTATTAAACGTGCTGAAGATGTAATTAACCGTTACTTATTACCTCACATGGGTATT
GAATCAGAAAGACGTGAAGCTAAAGCTACTTATTTAGCTGAAATGACTGAAATGTTGCTTCAGGTTATTGATGAACAAAG
AGAACCTCACGATAAGGACCATTACACAAATAAAAGACTCAGAGTATCTGGAGATTTAATGGAAGATTTATTCAGAGTTG
CTTTTACAAGTTTAACCAGAGATATGAGTTATCAACTTGAAAGAAGTATTTCCCGTGGTAAAGAGCTTTCAATCAAACAG
GCAGTTCGTAGTGATGTTTTAACTGAAAATATTAAACATGCTATTGCAACTGGTAACTGGGTTGGTGGAAGAGCAGGTGT
AAGTCAATTATTAGATAGGACTAGTTATATGGGAACTCTTTCTCATTTAAGACGTGTTGTATCTCCTTTAACAAGAAGTC
AACCTCACTTTGAAGCAAGGGATTTGCACCCAACACAATTTGGTAAGATTTGTCCGAATGAAACCCCAGAGGGTCCTAAC
TGTGGTCTGGTAAAGAATTTAGCTTTAATGTGTAATATTTCTGAAGGTTCTGATGAACAAGAAATCATTGATGTTATTAA
AAAGATGAATGTTTTAGAGGATTAA

Upstream 100 bases:

>100_bases
AGAATAATAATCAAATGAAGAATTTTAATTAGGTTTCCTAATCTTATTTTTATTTAGTTAATTATTTTTAAGGTGTTTTA
TAGAATGCTGGAGGAAGTCC

Downstream 100 bases:

>100_bases
AGGAACGATAATATGGATAGTGCTAAAATTTATATTAATGGAGAACTTTTAGGATATTGTAAAGACCCTGAAAGTTTCAC
CAATGAAATGAGAGAAAAAA

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTENNWKLVDAFFDKYDLVDHHIKSYNDFINSGIQNIIDITEPITLENGKYTLKTGKLFIEKPFTKEADGSKSMIYPAEA
RLRNLNYSAHMYLEMALNEEGEDNPLEKVYIGELPVMLKSDICHLHGLSDEELIEQGEDPQDLGGYFIVNGSERAVVTME
EIAPNKIILERIGDVEDRRAKAIVTSIKSGFRARISLEYKKPRKSGVFLRISFPYVPGEIPLVILLRALGLATGQEIITA
ISNDPNFQMIIADDIQVSLSQLKLDQKEMDGMDKEERRQYLQLAAIKYIGNRVAKGMTEEYRIKRAEDVINRYLLPHMGI
ESERREAKATYLAEMTEMLLQVIDEQREPHDKDHYTNKRLRVSGDLMEDLFRVAFTSLTRDMSYQLERSISRGKELSIKQ
AVRSDVLTENIKHAIATGNWVGGRAGVSQLLDRTSYMGTLSHLRRVVSPLTRSQPHFEARDLHPTQFGKICPNETPEGPN
CGLVKNLALMCNISEGSDEQEIIDVIKKMNVLED

Sequences:

>Translated_514_residues
MTENNWKLVDAFFDKYDLVDHHIKSYNDFINSGIQNIIDITEPITLENGKYTLKTGKLFIEKPFTKEADGSKSMIYPAEA
RLRNLNYSAHMYLEMALNEEGEDNPLEKVYIGELPVMLKSDICHLHGLSDEELIEQGEDPQDLGGYFIVNGSERAVVTME
EIAPNKIILERIGDVEDRRAKAIVTSIKSGFRARISLEYKKPRKSGVFLRISFPYVPGEIPLVILLRALGLATGQEIITA
ISNDPNFQMIIADDIQVSLSQLKLDQKEMDGMDKEERRQYLQLAAIKYIGNRVAKGMTEEYRIKRAEDVINRYLLPHMGI
ESERREAKATYLAEMTEMLLQVIDEQREPHDKDHYTNKRLRVSGDLMEDLFRVAFTSLTRDMSYQLERSISRGKELSIKQ
AVRSDVLTENIKHAIATGNWVGGRAGVSQLLDRTSYMGTLSHLRRVVSPLTRSQPHFEARDLHPTQFGKICPNETPEGPN
CGLVKNLALMCNISEGSDEQEIIDVIKKMNVLED
>Mature_513_residues
TENNWKLVDAFFDKYDLVDHHIKSYNDFINSGIQNIIDITEPITLENGKYTLKTGKLFIEKPFTKEADGSKSMIYPAEAR
LRNLNYSAHMYLEMALNEEGEDNPLEKVYIGELPVMLKSDICHLHGLSDEELIEQGEDPQDLGGYFIVNGSERAVVTMEE
IAPNKIILERIGDVEDRRAKAIVTSIKSGFRARISLEYKKPRKSGVFLRISFPYVPGEIPLVILLRALGLATGQEIITAI
SNDPNFQMIIADDIQVSLSQLKLDQKEMDGMDKEERRQYLQLAAIKYIGNRVAKGMTEEYRIKRAEDVINRYLLPHMGIE
SERREAKATYLAEMTEMLLQVIDEQREPHDKDHYTNKRLRVSGDLMEDLFRVAFTSLTRDMSYQLERSISRGKELSIKQA
VRSDVLTENIKHAIATGNWVGGRAGVSQLLDRTSYMGTLSHLRRVVSPLTRSQPHFEARDLHPTQFGKICPNETPEGPNC
GLVKNLALMCNISEGSDEQEIIDVIKKMNVLED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=545, Percent_Identity=34.3119266055046, Blast_Score=315, Evalue=9e-86,
Organism=Homo sapiens, GI238908503, Length=525, Percent_Identity=29.3333333333333, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI238908505, Length=475, Percent_Identity=28.6315789473684, Blast_Score=208, Evalue=8e-54,
Organism=Homo sapiens, GI33469941, Length=514, Percent_Identity=21.5953307392996, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1790419, Length=168, Percent_Identity=29.7619047619048, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552304, Length=544, Percent_Identity=32.9044117647059, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25144348, Length=515, Percent_Identity=28.3495145631068, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17506623, Length=531, Percent_Identity=24.4821092278719, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324725, Length=562, Percent_Identity=34.1637010676157, Blast_Score=310, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6324781, Length=518, Percent_Identity=30.5019305019305, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6325267, Length=545, Percent_Identity=24.5871559633028, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17136444, Length=547, Percent_Identity=34.7349177330896, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI17647877, Length=512, Percent_Identity=28.515625, Blast_Score=214, Evalue=9e-56,
Organism=Drosophila melanogaster, GI17136446, Length=525, Percent_Identity=21.9047619047619, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645 [H]

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 58514; Mature: 58383

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENNWKLVDAFFDKYDLVDHHIKSYNDFINSGIQNIIDITEPITLENGKYTLKTGKLFI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEECEEEE
EKPFTKEADGSKSMIYPAEARLRNLNYSAHMYLEMALNEEGEDNPLEKVYIGELPVMLKS
ECCCCCCCCCCCCEEECHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCHHHHH
DICHLHGLSDEELIEQGEDPQDLGGYFIVNGSERAVVTMEEIAPNKIILERIGDVEDRRA
CHHHHCCCCHHHHHHCCCCHHHCCCEEEECCCCCEEEEHHHHCCCHHHHHHHCCCHHHHH
KAIVTSIKSGFRARISLEYKKPRKSGVFLRISFPYVPGEIPLVILLRALGLATGQEIITA
HHHHHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHH
ISNDPNFQMIIADDIQVSLSQLKLDQKEMDGMDKEERRQYLQLAAIKYIGNRVAKGMTEE
HCCCCCEEEEEECCHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
YRIKRAEDVINRYLLPHMGIESERREAKATYLAEMTEMLLQVIDEQREPHDKDHYTNKRL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEE
RVSGDLMEDLFRVAFTSLTRDMSYQLERSISRGKELSIKQAVRSDVLTENIKHAIATGNW
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
VGGRAGVSQLLDRTSYMGTLSHLRRVVSPLTRSQPHFEARDLHPTQFGKICPNETPEGPN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
CGLVKNLALMCNISEGSDEQEIIDVIKKMNVLED
CCHHHCEEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TENNWKLVDAFFDKYDLVDHHIKSYNDFINSGIQNIIDITEPITLENGKYTLKTGKLFI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEECEEEE
EKPFTKEADGSKSMIYPAEARLRNLNYSAHMYLEMALNEEGEDNPLEKVYIGELPVMLKS
ECCCCCCCCCCCCEEECHHHHHHCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCHHHHH
DICHLHGLSDEELIEQGEDPQDLGGYFIVNGSERAVVTMEEIAPNKIILERIGDVEDRRA
CHHHHCCCCHHHHHHCCCCHHHCCCEEEECCCCCEEEEHHHHCCCHHHHHHHCCCHHHHH
KAIVTSIKSGFRARISLEYKKPRKSGVFLRISFPYVPGEIPLVILLRALGLATGQEIITA
HHHHHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHH
ISNDPNFQMIIADDIQVSLSQLKLDQKEMDGMDKEERRQYLQLAAIKYIGNRVAKGMTEE
HCCCCCEEEEEECCHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
YRIKRAEDVINRYLLPHMGIESERREAKATYLAEMTEMLLQVIDEQREPHDKDHYTNKRL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEE
RVSGDLMEDLFRVAFTSLTRDMSYQLERSISRGKELSIKQAVRSDVLTENIKHAIATGNW
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
VGGRAGVSQLLDRTSYMGTLSHLRRVVSPLTRSQPHFEARDLHPTQFGKICPNETPEGPN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
CGLVKNLALMCNISEGSDEQEIIDVIKKMNVLED
CCHHHCEEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]