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The map label for this gene is rpoB1 [H]

Identifier: 148642970

GI number: 148642970

Start: 874332

End: 876143

Strand: Reverse

Name: rpoB1 [H]

Synonym: Msm_0910

Alternate gene names: 148642970

Gene position: 876143-874332 (Counterclockwise)

Preceding gene: 148642971

Following gene: 148642969

Centisome position: 47.28

GC content: 35.65

Gene sequence:

>1812_bases
ATGGATAGTGCTAAAATTTATATTAATGGAGAACTTTTAGGATATTGTAAAGACCCTGAAAGTTTCACCAATGAAATGAG
AGAAAAAAGGAGAAATGGTGAGGTTTCTCATGAAATGAATATTACTTATTATGATAAAAATAATGAGATTTATATATTCA
ATGACCCAGGTAGGGCAAGAAGACCATTAATCATTGTTAAAGATGGTAAACCAGTTCTTAGTGATAAACATTTAGAAAAA
GTAGCTAAAGGCGAATTAAAATGGGATGATTTAATAGACAATGGTTTTATTGAATACTTGGATGCTGAAGAGGAAGAAAA
CTCCTATATTGCTATGAGTATTGCTGATTTAAATGAGGAACATACTCATTTGGAAATTGACCCGGCTACTATGCTTGGAA
TTTGTGCAGGTATTATTCCGTTTTCCGATCACAATTCTTCACCAAGGAATACTATGGAAGCAGGTATGACAAAACAAGCA
TTAGGATTATATGTTTCTAACTATGCATTACGTACAGATACAAGGGCTCACTTATTACACCACCCGCAAACTCCTATTGT
AAAAACACGTATTATTGATTCAACTAATTATGACAAAAGACCATCAGGTCAGAACTTTGTTGTAGCTCTTATGTCTTACG
AAGGATATAACATGGAAGATGCAATGGTTATTAACAAAGGTTCTCTTGAAAGAGGATTGGCTAGGTCTTCATTTTTCAGA
GCTTATGATACTGCTGAAAAAAGGTATCCTGGTGGACAAGAGGATAAATTTGAGGTTCCTGATAAAAACATTAAAGGTTA
CCGTTCCGAAGATGCTTATAGGCACTTAGATGATGACGGTGTTGTTAACCCGGAATCATATGTTGAATCTGGTGATGTAT
TAATCGGTAAAACTTCACCACCAAGATTCTTGGAAGAAATCGACGAATTCGGAACTGTAGCTGAAAAAAGAAGGGAAACT
TCTGTAACAGTAAGACACGGTGAAAAAGGTATCGTTGATGCTGTTCTTTTAACTGAAACTGTTGAAGGAAGCAGATTGGC
TAAAATTAGAGTAAGAGATACCAGGCAACCTGAATTTGGGGATAAATTTGCATCAAGGCACGGTCAGAAAGGGGTTCTTG
GTTTAATATTATCTCCTGAAGATGTTCCTTTCACAGAATCTGGTGTTGTTCCTGATTTAATTGTTAACCCTCACGCTATT
CCTTCCAGGATGTCTGTAGGACAAGTGCTTGAAATGGTAGCAGGTAAAGCAGGCTGTCTTGAAGGTGAACGTGTTGACGG
AACTCCATTCAATACAGAACTTGAAAAAGAAATCAAACAACAGCTTAAAAATCACGGATTTGAATCTGCAGGTTGCGAAT
CATTATATAATGGTGTAACCGGAGAAAGAATCGAAGCTGAAATATTTGTTGGTGTGGCATATTACCAAAAATTACACCAC
ATGACTACTGATAAAGTTTATGCTCGTTCAAGAGGTCCTGTACAAGTTCTTACCCGTCAGCCTACTGAAGGAAGAGCTCG
TGAAGGTGGTTTAAGGTTTGGAGAAATGGAAAGAGATTGTCTTATTGCACACGGTGCAGCTCTTACCTTAAAAGAAAGAC
TTTTAGATGAGTCTGACAAATATGAAGCTATTGTCTGTGAAAATTGTGGTATGTTAGCAGTATATGATAAGAATAAAAAT
AAAAAGTACTGTCCAATTTGTGGAGATGTTGAAACTTATCCGATAGAAATTTCATACGCATTTAAATTATTATTAGATGA
ACTTAAGAGTTTATGTATTTTCCCTAAATTAGTTTTAGGAGACAAAACATAA

Upstream 100 bases:

>100_bases
TTTAGCTTTAATGTGTAATATTTCTGAAGGTTCTGATGAACAAGAAATCATTGATGTTATTAAAAAGATGAATGTTTTAG
AGGATTAAAGGAACGATAAT

Downstream 100 bases:

>100_bases
TTATATAATATGAAGGAGTCAATATTTTGAAAGGATTTATAAAAAAAATCAAACAAATTAACTTTGGGCTTATGTCTCCT
GAGGATATTCGTGAAATGTC

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MDSAKIYINGELLGYCKDPESFTNEMREKRRNGEVSHEMNITYYDKNNEIYIFNDPGRARRPLIIVKDGKPVLSDKHLEK
VAKGELKWDDLIDNGFIEYLDAEEEENSYIAMSIADLNEEHTHLEIDPATMLGICAGIIPFSDHNSSPRNTMEAGMTKQA
LGLYVSNYALRTDTRAHLLHHPQTPIVKTRIIDSTNYDKRPSGQNFVVALMSYEGYNMEDAMVINKGSLERGLARSSFFR
AYDTAEKRYPGGQEDKFEVPDKNIKGYRSEDAYRHLDDDGVVNPESYVESGDVLIGKTSPPRFLEEIDEFGTVAEKRRET
SVTVRHGEKGIVDAVLLTETVEGSRLAKIRVRDTRQPEFGDKFASRHGQKGVLGLILSPEDVPFTESGVVPDLIVNPHAI
PSRMSVGQVLEMVAGKAGCLEGERVDGTPFNTELEKEIKQQLKNHGFESAGCESLYNGVTGERIEAEIFVGVAYYQKLHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALTLKERLLDESDKYEAIVCENCGMLAVYDKNKN
KKYCPICGDVETYPIEISYAFKLLLDELKSLCIFPKLVLGDKT

Sequences:

>Translated_603_residues
MDSAKIYINGELLGYCKDPESFTNEMREKRRNGEVSHEMNITYYDKNNEIYIFNDPGRARRPLIIVKDGKPVLSDKHLEK
VAKGELKWDDLIDNGFIEYLDAEEEENSYIAMSIADLNEEHTHLEIDPATMLGICAGIIPFSDHNSSPRNTMEAGMTKQA
LGLYVSNYALRTDTRAHLLHHPQTPIVKTRIIDSTNYDKRPSGQNFVVALMSYEGYNMEDAMVINKGSLERGLARSSFFR
AYDTAEKRYPGGQEDKFEVPDKNIKGYRSEDAYRHLDDDGVVNPESYVESGDVLIGKTSPPRFLEEIDEFGTVAEKRRET
SVTVRHGEKGIVDAVLLTETVEGSRLAKIRVRDTRQPEFGDKFASRHGQKGVLGLILSPEDVPFTESGVVPDLIVNPHAI
PSRMSVGQVLEMVAGKAGCLEGERVDGTPFNTELEKEIKQQLKNHGFESAGCESLYNGVTGERIEAEIFVGVAYYQKLHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALTLKERLLDESDKYEAIVCENCGMLAVYDKNKN
KKYCPICGDVETYPIEISYAFKLLLDELKSLCIFPKLVLGDKT
>Mature_603_residues
MDSAKIYINGELLGYCKDPESFTNEMREKRRNGEVSHEMNITYYDKNNEIYIFNDPGRARRPLIIVKDGKPVLSDKHLEK
VAKGELKWDDLIDNGFIEYLDAEEEENSYIAMSIADLNEEHTHLEIDPATMLGICAGIIPFSDHNSSPRNTMEAGMTKQA
LGLYVSNYALRTDTRAHLLHHPQTPIVKTRIIDSTNYDKRPSGQNFVVALMSYEGYNMEDAMVINKGSLERGLARSSFFR
AYDTAEKRYPGGQEDKFEVPDKNIKGYRSEDAYRHLDDDGVVNPESYVESGDVLIGKTSPPRFLEEIDEFGTVAEKRRET
SVTVRHGEKGIVDAVLLTETVEGSRLAKIRVRDTRQPEFGDKFASRHGQKGVLGLILSPEDVPFTESGVVPDLIVNPHAI
PSRMSVGQVLEMVAGKAGCLEGERVDGTPFNTELEKEIKQQLKNHGFESAGCESLYNGVTGERIEAEIFVGVAYYQKLHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALTLKERLLDESDKYEAIVCENCGMLAVYDKNKN
KKYCPICGDVETYPIEISYAFKLLLDELKSLCIFPKLVLGDKT

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=612, Percent_Identity=43.6274509803922, Blast_Score=535, Evalue=1e-152,
Organism=Homo sapiens, GI238908503, Length=600, Percent_Identity=42.6666666666667, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI238908505, Length=600, Percent_Identity=42.6666666666667, Blast_Score=501, Evalue=1e-142,
Organism=Homo sapiens, GI33469941, Length=514, Percent_Identity=37.5486381322957, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI212286172, Length=514, Percent_Identity=37.5486381322957, Blast_Score=331, Evalue=2e-90,
Organism=Escherichia coli, GI1790419, Length=303, Percent_Identity=31.3531353135314, Blast_Score=133, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17552304, Length=612, Percent_Identity=42.3202614379085, Blast_Score=530, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI25144348, Length=608, Percent_Identity=40.953947368421, Blast_Score=493, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17506623, Length=640, Percent_Identity=32.8125, Blast_Score=297, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6324725, Length=645, Percent_Identity=43.7209302325581, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6324781, Length=604, Percent_Identity=43.7086092715232, Blast_Score=501, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6325267, Length=479, Percent_Identity=38.6221294363257, Blast_Score=315, Evalue=1e-86,
Organism=Drosophila melanogaster, GI17136444, Length=614, Percent_Identity=44.1368078175896, Blast_Score=535, Evalue=1e-152,
Organism=Drosophila melanogaster, GI17647877, Length=602, Percent_Identity=41.3621262458472, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17136446, Length=625, Percent_Identity=32.8, Blast_Score=316, Evalue=3e-86,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 67778; Mature: 67778

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01166 RNA_POL_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSAKIYINGELLGYCKDPESFTNEMREKRRNGEVSHEMNITYYDKNNEIYIFNDPGRAR
CCCCEEEECCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEECCCCCCC
RPLIIVKDGKPVLSDKHLEKVAKGELKWDDLIDNGFIEYLDAEEEENSYIAMSIADLNEE
CCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCC
HTHLEIDPATMLGICAGIIPFSDHNSSPRNTMEAGMTKQALGLYVSNYALRTDTRAHLLH
CCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHCEEEECCCHHHHHC
HPQTPIVKTRIIDSTNYDKRPSGQNFVVALMSYEGYNMEDAMVINKGSLERGLARSSFFR
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHH
AYDTAEKRYPGGQEDKFEVPDKNIKGYRSEDAYRHLDDDGVVNPESYVESGDVLIGKTSP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHCCCCEEEECCCC
PRFLEEIDEFGTVAEKRRETSVTVRHGEKGIVDAVLLTETVEGSRLAKIRVRDTRQPEFG
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCC
DKFASRHGQKGVLGLILSPEDVPFTESGVVPDLIVNPHAIPSRMSVGQVLEMVAGKAGCL
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCC
EGERVDGTPFNTELEKEIKQQLKNHGFESAGCESLYNGVTGERIEAEIFVGVAYYQKLHH
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEEEHHHHHHHHHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALTLKERLLDESDK
CCHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCC
YEAIVCENCGMLAVYDKNKNKKYCPICGDVETYPIEISYAFKLLLDELKSLCIFPKLVLG
CCEEEECCCCEEEEEECCCCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCHHHCC
DKT
CCC
>Mature Secondary Structure
MDSAKIYINGELLGYCKDPESFTNEMREKRRNGEVSHEMNITYYDKNNEIYIFNDPGRAR
CCCCEEEECCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEECCCCCCC
RPLIIVKDGKPVLSDKHLEKVAKGELKWDDLIDNGFIEYLDAEEEENSYIAMSIADLNEE
CCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCC
HTHLEIDPATMLGICAGIIPFSDHNSSPRNTMEAGMTKQALGLYVSNYALRTDTRAHLLH
CCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHCEEEECCCHHHHHC
HPQTPIVKTRIIDSTNYDKRPSGQNFVVALMSYEGYNMEDAMVINKGSLERGLARSSFFR
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHH
AYDTAEKRYPGGQEDKFEVPDKNIKGYRSEDAYRHLDDDGVVNPESYVESGDVLIGKTSP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHCCCCEEEECCCC
PRFLEEIDEFGTVAEKRRETSVTVRHGEKGIVDAVLLTETVEGSRLAKIRVRDTRQPEFG
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCC
DKFASRHGQKGVLGLILSPEDVPFTESGVVPDLIVNPHAIPSRMSVGQVLEMVAGKAGCL
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCC
EGERVDGTPFNTELEKEIKQQLKNHGFESAGCESLYNGVTGERIEAEIFVGVAYYQKLHH
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEEEHHHHHHHHHH
MTTDKVYARSRGPVQVLTRQPTEGRAREGGLRFGEMERDCLIAHGAALTLKERLLDESDK
CCHHHHHHCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCC
YEAIVCENCGMLAVYDKNKNKKYCPICGDVETYPIEISYAFKLLLDELKSLCIFPKLVLG
CCEEEECCCCEEEEEECCCCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCHHHCC
DKT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2843811; 2834336 [H]