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The map label for this gene is gdh [H]

Identifier: 148642948

GI number: 148642948

Start: 849751

End: 851094

Strand: Reverse

Name: gdh [H]

Synonym: Msm_0888

Alternate gene names: 148642948

Gene position: 851094-849751 (Counterclockwise)

Preceding gene: 148642951

Following gene: 148642947

Centisome position: 45.93

GC content: 35.94

Gene sequence:

>1344_bases
ATGTCTTACGTAGATGATGTAATAGAATTAACTGTAAAACAGAACCCTTCTGAACCAGAATTCCATCAGGCTGTAAAAGA
AGTATTGGAATCTTTAAGGGTTGTTATTGAAGCAAATGAAGAAGAATATAAAAAGAATGCACTTCTTGAAAGGTTAGTTA
ACCCGGAAAGACAATTAAAATTCCGTGTTCCATGGGTAGATGACAATGGACAAGTACAAGTAAATACTGGATACCGTGTA
CAATTCAACAGTGCAATTGGGCCTTACAAAGGTGGATTACGTTTCCACCCTTCAGTAAATGTGGGTATTATTAAATTCTT
AGGTTTTGAACAAATTTTCAAAAACTCCTTAACAGGCCTTGCAATTGGTGGAGGTAAAGGAGGTTCCGACTTTGATCCTA
AAGGAAAATCTGACAGGGAAATCATGGCATTCTGTCAAAGTTTCATGACTGAATTATTCAAATACATTGGTGCAGACACT
GATGTTCCAGCAGGAGATATTGGTGTTGGTGGTAGAGAAATTGGTTTCTTATATGGTCAATACAAAAGGATTAGGGGATT
ATCTGAAGGAGTATTAACTGGTAAAGCATTATCTTATGGTGGTTCATTAGCAAGAACTGAAGCTACAGGATACGGATTAT
TATACTTTACTGATGCAATGTTAAAAGCAAATGACATTGATATTAAAGGAAAAACTATTGCAGTATCCGGTGCAGGTAAT
GTAGCTATTTATGCTATTGAAAAAGCACAACAATTAGGCGGTAATCCTGTAACCTGTTCTGATTCTACCGGTTGGATTTA
TGATCCTGAAGGAATTGATGTAGAATTATTAAAAGAAGTAAAAGAAGTAAAAGAAGTAAAACGTGCAAGACTAACTGAAT
ATGCTGAAGCAAGACCAAGTGCAGAATATCACGAAGGTAAAGGTGTATGGTCTGTTAAATGTGATATTGCTCTTCCATGT
GCAACTCAAAACGAATTACTTTTAGATGATGCTAAACAGTTAGTAGCTAATGGTGTTGTTGCAGTTGCTGAAGGTGCAAA
CATGCCTACTTCTATTGAAGCTACTGAATACTTACAAGACAATGATGTATTATTTGGACCAGGAAAAGCTTCCAATGCTG
GTGGTGTAGCTACTTCTGCATTAGAAATGGCTCAAAACTCTCAAAGATTCTCATGGGACTTTGATAAAGTTGATAAAAGA
CTTAAAGTCATTATGGAAAACATCTTTGCTAATGTTGATGAAGCAGCTAAAACCTATGGATTTGAGAAAAATTACGTAGT
CGGTGCAAACATAGCTGGTTTTGAAAAAGTAGTAGATGCAATGAATGCTCAAGGAATCGTTTAG

Upstream 100 bases:

>100_bases
TAGAAAATATTATTATTTATTCAAATAGATTTTTGAATTTTTTAATCAATTTCTAGAAAATTCCGTAATTATATATTTTA
ATTAACGGAGGAGAAATATT

Downstream 100 bases:

>100_bases
ATTTATTTAACTTGGCTATAGTTAATTCTATAGCTTTTAACTTTTTTTTAAGAAAAATTTCATAAAAACATTGTTTTTTG
TCTATTTGACTGGCTAAATT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSYVDDVIELTVKQNPSEPEFHQAVKEVLESLRVVIEANEEEYKKNALLERLVNPERQLKFRVPWVDDNGQVQVNTGYRV
QFNSAIGPYKGGLRFHPSVNVGIIKFLGFEQIFKNSLTGLAIGGGKGGSDFDPKGKSDREIMAFCQSFMTELFKYIGADT
DVPAGDIGVGGREIGFLYGQYKRIRGLSEGVLTGKALSYGGSLARTEATGYGLLYFTDAMLKANDIDIKGKTIAVSGAGN
VAIYAIEKAQQLGGNPVTCSDSTGWIYDPEGIDVELLKEVKEVKEVKRARLTEYAEARPSAEYHEGKGVWSVKCDIALPC
ATQNELLLDDAKQLVANGVVAVAEGANMPTSIEATEYLQDNDVLFGPGKASNAGGVATSALEMAQNSQRFSWDFDKVDKR
LKVIMENIFANVDEAAKTYGFEKNYVVGANIAGFEKVVDAMNAQGIV

Sequences:

>Translated_447_residues
MSYVDDVIELTVKQNPSEPEFHQAVKEVLESLRVVIEANEEEYKKNALLERLVNPERQLKFRVPWVDDNGQVQVNTGYRV
QFNSAIGPYKGGLRFHPSVNVGIIKFLGFEQIFKNSLTGLAIGGGKGGSDFDPKGKSDREIMAFCQSFMTELFKYIGADT
DVPAGDIGVGGREIGFLYGQYKRIRGLSEGVLTGKALSYGGSLARTEATGYGLLYFTDAMLKANDIDIKGKTIAVSGAGN
VAIYAIEKAQQLGGNPVTCSDSTGWIYDPEGIDVELLKEVKEVKEVKRARLTEYAEARPSAEYHEGKGVWSVKCDIALPC
ATQNELLLDDAKQLVANGVVAVAEGANMPTSIEATEYLQDNDVLFGPGKASNAGGVATSALEMAQNSQRFSWDFDKVDKR
LKVIMENIFANVDEAAKTYGFEKNYVVGANIAGFEKVVDAMNAQGIV
>Mature_446_residues
SYVDDVIELTVKQNPSEPEFHQAVKEVLESLRVVIEANEEEYKKNALLERLVNPERQLKFRVPWVDDNGQVQVNTGYRVQ
FNSAIGPYKGGLRFHPSVNVGIIKFLGFEQIFKNSLTGLAIGGGKGGSDFDPKGKSDREIMAFCQSFMTELFKYIGADTD
VPAGDIGVGGREIGFLYGQYKRIRGLSEGVLTGKALSYGGSLARTEATGYGLLYFTDAMLKANDIDIKGKTIAVSGAGNV
AIYAIEKAQQLGGNPVTCSDSTGWIYDPEGIDVELLKEVKEVKEVKRARLTEYAEARPSAEYHEGKGVWSVKCDIALPCA
TQNELLLDDAKQLVANGVVAVAEGANMPTSIEATEYLQDNDVLFGPGKASNAGGVATSALEMAQNSQRFSWDFDKVDKRL
KVIMENIFANVDEAAKTYGFEKNYVVGANIAGFEKVVDAMNAQGIV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=369, Percent_Identity=30.6233062330623, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4885281, Length=383, Percent_Identity=29.7650130548303, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1788059, Length=436, Percent_Identity=59.4036697247706, Blast_Score=515, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17544676, Length=412, Percent_Identity=27.1844660194175, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319256, Length=457, Percent_Identity=51.2035010940919, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324951, Length=454, Percent_Identity=50.4405286343612, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553475, Length=390, Percent_Identity=29.7435897435897, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45549226, Length=390, Percent_Identity=29.7435897435897, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24649283, Length=371, Percent_Identity=30.7277628032345, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=30.952380952381, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48641; Mature: 48509

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYVDDVIELTVKQNPSEPEFHQAVKEVLESLRVVIEANEEEYKKNALLERLVNPERQLK
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCHHEE
FRVPWVDDNGQVQVNTGYRVQFNSAIGPYKGGLRFHPSVNVGIIKFLGFEQIFKNSLTGL
EECCEECCCCEEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHCHHHHHHCCCCEE
AIGGGKGGSDFDPKGKSDREIMAFCQSFMTELFKYIGADTDVPAGDIGVGGREIGFLYGQ
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
YKRIRGLSEGVLTGKALSYGGSLARTEATGYGLLYFTDAMLKANDIDIKGKTIAVSGAGN
HHHHCCCHHCHHCCCHHHCCCCEEEECCCCCEEEEEEHHHHCCCCCEECCCEEEEECCCC
VAIYAIEKAQQLGGNPVTCSDSTGWIYDPEGIDVELLKEVKEVKEVKRARLTEYAEARPS
EEEEEEEHHHHCCCCCEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEYHEGKGVWSVKCDIALPCATQNELLLDDAKQLVANGVVAVAEGANMPTSIEATEYLQD
CCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCC
NDVLFGPGKASNAGGVATSALEMAQNSQRFSWDFDKVDKRLKVIMENIFANVDEAAKTYG
CCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCC
FEKNYVVGANIAGFEKVVDAMNAQGIV
CCCCEEEECCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SYVDDVIELTVKQNPSEPEFHQAVKEVLESLRVVIEANEEEYKKNALLERLVNPERQLK
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCHHEE
FRVPWVDDNGQVQVNTGYRVQFNSAIGPYKGGLRFHPSVNVGIIKFLGFEQIFKNSLTGL
EECCEECCCCEEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHCHHHHHHCCCCEE
AIGGGKGGSDFDPKGKSDREIMAFCQSFMTELFKYIGADTDVPAGDIGVGGREIGFLYGQ
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
YKRIRGLSEGVLTGKALSYGGSLARTEATGYGLLYFTDAMLKANDIDIKGKTIAVSGAGN
HHHHCCCHHCHHCCCHHHCCCCEEEECCCCCEEEEEEHHHHCCCCCEECCCEEEEECCCC
VAIYAIEKAQQLGGNPVTCSDSTGWIYDPEGIDVELLKEVKEVKEVKRARLTEYAEARPS
EEEEEEEHHHHCCCCCEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEYHEGKGVWSVKCDIALPCATQNELLLDDAKQLVANGVVAVAEGANMPTSIEATEYLQD
CCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCC
NDVLFGPGKASNAGGVATSALEMAQNSQRFSWDFDKVDKRLKVIMENIFANVDEAAKTYG
CCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCC
FEKNYVVGANIAGFEKVVDAMNAQGIV
CCCCEEEECCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]