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The map label for this gene is putP [H]

Identifier: 148642446

GI number: 148642446

Start: 367505

End: 369118

Strand: Reverse

Name: putP [H]

Synonym: Msm_0386

Alternate gene names: 148642446

Gene position: 369118-367505 (Counterclockwise)

Preceding gene: 148642449

Following gene: 148642444

Centisome position: 19.92

GC content: 36.31

Gene sequence:

>1614_bases
ATGGACATTATGATTTTAGCGATTGTATTTATAATCTACATTTTTGCTTTAGTATTGGTAGGTTATTATGCATGGAAAGG
AACAGAATCCTCAGAGGATTTCATGATTGGTGGGAAGGATACACATCCTGCGATCATGGCTTTAAGTTATGGAGCAACTT
TTATTTCGACTGCAGCTATTGTCGGTTTTGGTGGAGTTGCTAGTGAATATGGTATGAGTGTTCTATGGTTGGCATTTTTA
AACATAATCATTGGGGTATTCATAGCTTTTGTATTTTTAGGTAAAAGAACCCGTAGAATGGGACACAGTTTAGGTTCCCT
AACGTTTCCCGAGTTCTTGGGCAAACGTTTTAATAGTAAATTTATTCATTATTTCTCAGGTTTAGTTATATTTTGTGCTA
TGCCGCTTTATGCAGCAGTAGTTCTTATTGGTGCTGCAAGATTCTTAGAATCTTCTTTAATGATTGATTTCGGAATATCC
TTATTTATTTTATCAGTTATAATTACATTCTATGTATTCTTTGGAGGTATTAAGGGAGTAATGTATACTGATGCGCTACA
GGGAGTAATTATGGTTTGTGCAATGGTATTTTTACTTGGATTTGTTTACTGGTTGCTTGGTGGTGTAACTCATGCAAACA
CAGCATTAACAGATATGGTGGGATTATATCCGGCTAGTGCTACAGCTACAGGGGGAACAGGTTGGACAACATTCCCTGAG
TTGGGTTCACCGTTTTGGTGGAGTTTGGTTTCATCTACTCTTATTGGTGTAGGTATTGGTGTATTGGCTCAACCTCAACT
GATTGTAAGATTTATGACTGTTAAATCTGACAGGGAATTAAACAGATCAGTTCTTGTTGGAGGATTATTTATTGCAATAA
TGCCTACTACAGCTTATATTGTAGGTTCACTTTCCAATGTGTATTTCTTTGATAAATTGGGAAAAATAGCTGTAGATGTT
GTTGGCGGAAATGTTGACAAAGTTATTCCAACATTTATTACGATGGCATTGCCTGAATGGTTTGTATATATTTTCCTGCT
ATCATTATTGGCAGCGGCAATGTCTACAATCAGTTCCCAGCTTCATACTCAGGGAACTGCATTTGGTGTAGATATCTATG
GTACTTTAAGAAATAAAGCTAAGCAGAAATCAGACCAAATTACAGTTACAAGACTTGGTATTCTTATAGCTATTATTTTA
GCTTTAGTATTGGCATTTTCTCTTCCGGGAAGTATTGTTGCTCTTGGAACAAGTCTGTTCTTTGAAATCTGTGCGGCTGC
ATTTTTACCTGTGTTCTTGGGAGCATTGTATTGGAAAGGAATCACTGCTAAAGGTGCTATTGCAGGTATTGTTTCCGGAA
CATTTGTTAGTTTATTCTGGTTACTCTTTGTATTTGCAAAAACAGCTAAAGGTTTAGGATTGTGTAAAGCAATATTTGGT
TGTGCAACTTTATTGCCGGCCATGCCATGGCCTTATATTGATGTAATGTTAATAGCTGTTCCTGTTTCTGCTATATTTAC
ATTGGTTGTTAGTTTACTTACAAAACCTCCTGCTCAAGAGGTTATTGATAAGGCATTTGAAAATATTGGCAACAAAGGAA
GTGAGGTACAATGA

Upstream 100 bases:

>100_bases
TTTCTTTGTTCTAATTTTTTAATATAAAATTATATATAACTATTCATATTAAAAATTTAATCATATATTAATTTTTTTTA
ATATATTCGGAGTTTAAATT

Downstream 100 bases:

>100_bases
TGGTTTTAGGAATTGAAGACCCTTGGATTTGGGGTGTTTATGTATTAATCATTTTATCCACTTTGTTATGCGTAGTCTAC
GGGATTGTGTATTGGAATAG

Product: sodium/proline symporter (proline permease), PutP

Products: NA

Alternate protein names: Proline permease [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MDIMILAIVFIIYIFALVLVGYYAWKGTESSEDFMIGGKDTHPAIMALSYGATFISTAAIVGFGGVASEYGMSVLWLAFL
NIIIGVFIAFVFLGKRTRRMGHSLGSLTFPEFLGKRFNSKFIHYFSGLVIFCAMPLYAAVVLIGAARFLESSLMIDFGIS
LFILSVIITFYVFFGGIKGVMYTDALQGVIMVCAMVFLLGFVYWLLGGVTHANTALTDMVGLYPASATATGGTGWTTFPE
LGSPFWWSLVSSTLIGVGIGVLAQPQLIVRFMTVKSDRELNRSVLVGGLFIAIMPTTAYIVGSLSNVYFFDKLGKIAVDV
VGGNVDKVIPTFITMALPEWFVYIFLLSLLAAAMSTISSQLHTQGTAFGVDIYGTLRNKAKQKSDQITVTRLGILIAIIL
ALVLAFSLPGSIVALGTSLFFEICAAAFLPVFLGALYWKGITAKGAIAGIVSGTFVSLFWLLFVFAKTAKGLGLCKAIFG
CATLLPAMPWPYIDVMLIAVPVSAIFTLVVSLLTKPPAQEVIDKAFENIGNKGSEVQ

Sequences:

>Translated_537_residues
MDIMILAIVFIIYIFALVLVGYYAWKGTESSEDFMIGGKDTHPAIMALSYGATFISTAAIVGFGGVASEYGMSVLWLAFL
NIIIGVFIAFVFLGKRTRRMGHSLGSLTFPEFLGKRFNSKFIHYFSGLVIFCAMPLYAAVVLIGAARFLESSLMIDFGIS
LFILSVIITFYVFFGGIKGVMYTDALQGVIMVCAMVFLLGFVYWLLGGVTHANTALTDMVGLYPASATATGGTGWTTFPE
LGSPFWWSLVSSTLIGVGIGVLAQPQLIVRFMTVKSDRELNRSVLVGGLFIAIMPTTAYIVGSLSNVYFFDKLGKIAVDV
VGGNVDKVIPTFITMALPEWFVYIFLLSLLAAAMSTISSQLHTQGTAFGVDIYGTLRNKAKQKSDQITVTRLGILIAIIL
ALVLAFSLPGSIVALGTSLFFEICAAAFLPVFLGALYWKGITAKGAIAGIVSGTFVSLFWLLFVFAKTAKGLGLCKAIFG
CATLLPAMPWPYIDVMLIAVPVSAIFTLVVSLLTKPPAQEVIDKAFENIGNKGSEVQ
>Mature_537_residues
MDIMILAIVFIIYIFALVLVGYYAWKGTESSEDFMIGGKDTHPAIMALSYGATFISTAAIVGFGGVASEYGMSVLWLAFL
NIIIGVFIAFVFLGKRTRRMGHSLGSLTFPEFLGKRFNSKFIHYFSGLVIFCAMPLYAAVVLIGAARFLESSLMIDFGIS
LFILSVIITFYVFFGGIKGVMYTDALQGVIMVCAMVFLLGFVYWLLGGVTHANTALTDMVGLYPASATATGGTGWTTFPE
LGSPFWWSLVSSTLIGVGIGVLAQPQLIVRFMTVKSDRELNRSVLVGGLFIAIMPTTAYIVGSLSNVYFFDKLGKIAVDV
VGGNVDKVIPTFITMALPEWFVYIFLLSLLAAAMSTISSQLHTQGTAFGVDIYGTLRNKAKQKSDQITVTRLGILIAIIL
ALVLAFSLPGSIVALGTSLFFEICAAAFLPVFLGALYWKGITAKGAIAGIVSGTFVSLFWLLFVFAKTAKGLGLCKAIFG
CATLLPAMPWPYIDVMLIAVPVSAIFTLVVSLLTKPPAQEVIDKAFENIGNKGSEVQ

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. Since most S.aureus strains are L- proline auxotrophs, this transporter may aid the bacterial persistence during an infection of tissues with low proline concentrations (By similarity) [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=532, Percent_Identity=31.7669172932331, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI14140236, Length=538, Percent_Identity=23.4200743494424, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI4507031, Length=463, Percent_Identity=26.133909287257, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI4507035, Length=373, Percent_Identity=24.1286863270777, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI110835708, Length=552, Percent_Identity=23.5507246376812, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI206597483, Length=451, Percent_Identity=25.7206208425721, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI206597487, Length=451, Percent_Identity=25.7206208425721, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI157671931, Length=454, Percent_Identity=24.2290748898678, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4507033, Length=574, Percent_Identity=23.1707317073171, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI17941285, Length=472, Percent_Identity=23.0932203389831, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1787251, Length=466, Percent_Identity=25.9656652360515, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082237, Length=462, Percent_Identity=25.974025974026, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1790503, Length=451, Percent_Identity=21.2860310421286, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790113, Length=398, Percent_Identity=25.1256281407035, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115533094, Length=388, Percent_Identity=24.4845360824742, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 57788; Mature: 57788

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA