Click here to switch to the map view.

The map label for this gene is pdxT

Identifier: 148642431

GI number: 148642431

Start: 352542

End: 353129

Strand: Reverse

Name: pdxT

Synonym: Msm_0371

Alternate gene names: 148642431

Gene position: 353129-352542 (Counterclockwise)

Preceding gene: 148642432

Following gene: 148642430

Centisome position: 19.06

GC content: 32.48

Gene sequence:

>588_bases
ATGATTACAATAGGAATACTAAATTTACAAGGTGCCGTAAGTGAACATTACGACATTACAAAGAAAGCTATTGAAAATAT
GGGTATTGAAGCAGAAGCAATTTCTGTAAGATATGCTGATGAAGTAGCGAACTGTGATGGTGTAATCATTTCTGGTGGTG
AAAGTACTGTGATAGGAAAAATCATCAAAGAACGAGGGATAGATAAAGTTATTAAAGATAACAGAATTCCTGTTTTTGGA
ACTTGCGCAGGAATGGTTTTGCTTGGTAAAAAAACTGATTTTGATCAGCCTTTATTGGGAATTATGGATATTTCAGTTAA
AAGGAATGCATTTGGAAGACAGGTAGATTCATTTGAAAGTCCAATTTCCATTTTAGGGGAAGATTATTTGGGAGTTTTCA
TTAGAGCACCATCCCTTGATGATTATGATAAAACCAAAGAAGATATTAAAGTTTTATCAAAATTGGATGATGAAATAATA
GCTATTCAGCAAGGACATAATATAGCTATTGCATTTCATCCGGAATTAACTGATGATACTAGAATTCACGAATATTTTAT
AGAGGAGGTTTTAAATTGTGTGGAATAG

Upstream 100 bases:

>100_bases
AGTATATTAGCAAATATTCTATATCTTAATAATTTTTACTATAACTTTTAGAATTACTGCTATTTTTTTAAAATAATGTT
TAGAATGATTAGTGAATTAT

Downstream 100 bases:

>100_bases
CTGGTATAGTTTATAAAGATAAAAAATTGCATTGTGCAGGTAATGACATGACCAATATGCTTCATCAACTTCAGCATAGA
GGTCCTGATTCTGCAGGTTA

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MITIGILNLQGAVSEHYDITKKAIENMGIEAEAISVRYADEVANCDGVIISGGESTVIGKIIKERGIDKVIKDNRIPVFG
TCAGMVLLGKKTDFDQPLLGIMDISVKRNAFGRQVDSFESPISILGEDYLGVFIRAPSLDDYDKTKEDIKVLSKLDDEII
AIQQGHNIAIAFHPELTDDTRIHEYFIEEVLNCVE

Sequences:

>Translated_195_residues
MITIGILNLQGAVSEHYDITKKAIENMGIEAEAISVRYADEVANCDGVIISGGESTVIGKIIKERGIDKVIKDNRIPVFG
TCAGMVLLGKKTDFDQPLLGIMDISVKRNAFGRQVDSFESPISILGEDYLGVFIRAPSLDDYDKTKEDIKVLSKLDDEII
AIQQGHNIAIAFHPELTDDTRIHEYFIEEVLNCVE
>Mature_195_residues
MITIGILNLQGAVSEHYDITKKAIENMGIEAEAISVRYADEVANCDGVIISGGESTVIGKIIKERGIDKVIKDNRIPVFG
TCAGMVLLGKKTDFDQPLLGIMDISVKRNAFGRQVDSFESPISILGEDYLGVFIRAPSLDDYDKTKEDIKVLSKLDDEII
AIQQGHNIAIAFHPELTDDTRIHEYFIEEVLNCVE

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=211, Percent_Identity=34.1232227488152, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323995, Length=216, Percent_Identity=35.1851851851852, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321048, Length=216, Percent_Identity=34.7222222222222, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_METS3 (A5UK48)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001272944.1
- ProteinModelPortal:   A5UK48
- SMR:   A5UK48
- STRING:   A5UK48
- GeneID:   5216526
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0371
- eggNOG:   arNOG08412
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13527
- HAMAP:   MF_01615
- InterPro:   IPR002161
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21542; Mature: 21542

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 82-82 ACT_SITE 173-173 ACT_SITE 175-175 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIGILNLQGAVSEHYDITKKAIENMGIEAEAISVRYADEVANCDGVIISGGESTVIGK
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCEEEEECCCCHHHHH
IIKERGIDKVIKDNRIPVFGTCAGMVLLGKKTDFDQPLLGIMDISVKRNAFGRQVDSFES
HHHHCCCHHHHHCCCCCEEEECCCEEEECCCCCCCCCCHHHEEEEECCCHHHCHHHHHCC
PISILGEDYLGVFIRAPSLDDYDKTKEDIKVLSKLDDEIIAIQQGHNIAIAFHPELTDDT
CHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCH
RIHEYFIEEVLNCVE
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MITIGILNLQGAVSEHYDITKKAIENMGIEAEAISVRYADEVANCDGVIISGGESTVIGK
CEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCEEEEECCCCHHHHH
IIKERGIDKVIKDNRIPVFGTCAGMVLLGKKTDFDQPLLGIMDISVKRNAFGRQVDSFES
HHHHCCCHHHHHCCCCCEEEECCCEEEECCCCCCCCCCHHHEEEEECCCHHHCHHHHHCC
PISILGEDYLGVFIRAPSLDDYDKTKEDIKVLSKLDDEIIAIQQGHNIAIAFHPELTDDT
CHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCH
RIHEYFIEEVLNCVE
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA