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The map label for this gene is endA [H]

Identifier: 148642277

GI number: 148642277

Start: 203517

End: 204032

Strand: Reverse

Name: endA [H]

Synonym: Msm_0217

Alternate gene names: 148642277

Gene position: 204032-203517 (Counterclockwise)

Preceding gene: 148642278

Following gene: 148642276

Centisome position: 11.01

GC content: 31.2

Gene sequence:

>516_bases
ATGCGCGGAAATTTATCTAATGATATTGTATCTATAAAAATTGAAGAAGGTAGTAAAAGACCGATAGCTTTACATGAAAA
AAGTCTATTTGGTAAAATTGAAGCAGATACTTTGAATCTTTCTTTAATTGAAGCTTGTTATTTACATGAGAAAGGTCGTT
TGGATGTTTATGAAGATGAAATCAAATGCAGTTTGGATTATCTTATTGACCTTTTGAAAAATAAGGAGATTTATGGAAAA
TACATTGTTTTTCGTGATTTGAAAGACCGTGGTTATGTTATTAAAACCGGATTTAAATATGGTTCAGATTTCCGTTTGTA
TAATCGTGGAGGAGGTCCTGGAAAAGGACATTCCGACTATCTTGTTAAAATTATTTATGAAACTTATGAGATTGATGCAC
TGGATTTCTCAAGTTATGTTAGAGTTTCTCATGGTGTTAACAAGAAACTTCTTCTGGCTATTGTAGATGACGATTTGGAT
ATTACATACTACAATGTTGAATGGACAAGACCATAA

Upstream 100 bases:

>100_bases
GAGTTTAGCTCTTATTTCTTTTTTTTTCTAAAGGTATATATAATACTGTAATCATAAATAACTCCATTAAATATAATTTT
TTTCTTTTGATGGTGTTTTA

Downstream 100 bases:

>100_bases
GTTTTAATTAAAGAAATGATTTATATTTTTATAGGTAATTAGATTTACCATTATAATTCTTAATTATATATTTAATTTAA
TGTATTTACTGTAGATAAGG

Product: tRNA-splicing endonuclease subunit alpha

Products: NA

Alternate protein names: tRNA-intron endonuclease [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MRGNLSNDIVSIKIEEGSKRPIALHEKSLFGKIEADTLNLSLIEACYLHEKGRLDVYEDEIKCSLDYLIDLLKNKEIYGK
YIVFRDLKDRGYVIKTGFKYGSDFRLYNRGGGPGKGHSDYLVKIIYETYEIDALDFSSYVRVSHGVNKKLLLAIVDDDLD
ITYYNVEWTRP

Sequences:

>Translated_171_residues
MRGNLSNDIVSIKIEEGSKRPIALHEKSLFGKIEADTLNLSLIEACYLHEKGRLDVYEDEIKCSLDYLIDLLKNKEIYGK
YIVFRDLKDRGYVIKTGFKYGSDFRLYNRGGGPGKGHSDYLVKIIYETYEIDALDFSSYVRVSHGVNKKLLLAIVDDDLD
ITYYNVEWTRP
>Mature_171_residues
MRGNLSNDIVSIKIEEGSKRPIALHEKSLFGKIEADTLNLSLIEACYLHEKGRLDVYEDEIKCSLDYLIDLLKNKEIYGK
YIVFRDLKDRGYVIKTGFKYGSDFRLYNRGGGPGKGHSDYLVKIIYETYEIDALDFSSYVRVSHGVNKKLLLAIVDDDLD
ITYYNVEWTRP

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- InterPro:   IPR016442 [H]

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N [H]

EC number: =3.1.27.9 [H]

Molecular weight: Translated: 19731; Mature: 19731

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGNLSNDIVSIKIEEGSKRPIALHEKSLFGKIEADTLNLSLIEACYLHEKGRLDVYEDE
CCCCCCCCEEEEEECCCCCCCEEEECCCCCCEEECCEECHHEEEEEHHCCCCCCEEEHHH
IKCSLDYLIDLLKNKEIYGKYIVFRDLKDRGYVIKTGFKYGSDFRLYNRGGGPGKGHSDY
HEEEHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCE
LVKIIYETYEIDALDFSSYVRVSHGVNKKLLLAIVDDDLDITYYNVEWTRP
EEEEEEEEEEEEEECCCCEEEECCCCCCEEEEEEECCCCEEEEEEEEECCC
>Mature Secondary Structure
MRGNLSNDIVSIKIEEGSKRPIALHEKSLFGKIEADTLNLSLIEACYLHEKGRLDVYEDE
CCCCCCCCEEEEEECCCCCCCEEEECCCCCCEEECCEECHHEEEEEHHCCCCCCEEEHHH
IKCSLDYLIDLLKNKEIYGKYIVFRDLKDRGYVIKTGFKYGSDFRLYNRGGGPGKGHSDY
HEEEHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCE
LVKIIYETYEIDALDFSSYVRVSHGVNKKLLLAIVDDDLDITYYNVEWTRP
EEEEEEEEEEEEEECCCCEEEECCCCCCEEEEEEECCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9200602; 9371463 [H]