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The map label for this gene is ppa [H]

Identifier: 148642258

GI number: 148642258

Start: 190663

End: 191070

Strand: Reverse

Name: ppa [H]

Synonym: Msm_0198

Alternate gene names: 148642258

Gene position: 191070-190663 (Counterclockwise)

Preceding gene: 148642259

Following gene: 148642257

Centisome position: 10.31

GC content: 34.56

Gene sequence:

>408_bases
ATGTTAGATAGAGTATTGTACTCTCCTGTTGTTTATCCGGCAGATTACGGTTTTATACCTAAATCTACATATGATGACGG
CGACCCTATGGATATTCTTGTTCTTATGGAACAACCTACATTCCCAGGATGTCTTATTGAAGCAAGACCTATTGGAATTA
TGGGTATGATTGACGGTGGAGACAAAGATTACAAAATCTTAGCTGTACCTGAAGACGATCCACGTTTCAGTGATGTTCAA
GACATCAGTGATGTTCCATCTCATTTATTAAAAGAAATTGAACACTTTTTCTCAGTTTATAAAAATCTTGAAGGAAAAGT
TGTTGAAACTAAAGGCTGGGAAGGTAAAGAGTCAGCTAAAAAAGAACTTTTAAGATCTTTAGAAATGTATAAAGAAAAAT
ACGAATAG

Upstream 100 bases:

>100_bases
TGAATCAGGTTCTGATATTCCTGATGTTGTAACAGCAGTTATTGAAGTGCCTAAAGGTTCAAGAAATAAATACGAATATG
ATAAGGAAAGAGAAGCTTTC

Downstream 100 bases:

>100_bases
AGGGATTGTTTTGTATTACATGACTAAAATTGAGGATACTGTAAGAATACCACCTTATAGATTTGATGATCCTCTTGAAG
AGGTAGCTATTCAAACTTTA

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MLDRVLYSPVVYPADYGFIPKSTYDDGDPMDILVLMEQPTFPGCLIEARPIGIMGMIDGGDKDYKILAVPEDDPRFSDVQ
DISDVPSHLLKEIEHFFSVYKNLEGKVVETKGWEGKESAKKELLRSLEMYKEKYE

Sequences:

>Translated_135_residues
MLDRVLYSPVVYPADYGFIPKSTYDDGDPMDILVLMEQPTFPGCLIEARPIGIMGMIDGGDKDYKILAVPEDDPRFSDVQ
DISDVPSHLLKEIEHFFSVYKNLEGKVVETKGWEGKESAKKELLRSLEMYKEKYE
>Mature_135_residues
MLDRVLYSPVVYPADYGFIPKSTYDDGDPMDILVLMEQPTFPGCLIEARPIGIMGMIDGGDKDYKILAVPEDDPRFSDVQ
DISDVPSHLLKEIEHFFSVYKNLEGKVVETKGWEGKESAKKELLRSLEMYKEKYE

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=136, Percent_Identity=39.7058823529412, Blast_Score=108, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319483, Length=139, Percent_Identity=28.0575539568345, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 15427; Mature: 15427

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRVLYSPVVYPADYGFIPKSTYDDGDPMDILVLMEQPTFPGCLIEARPIGIMGMIDGG
CCCCHHCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCEEEEEECCC
DKDYKILAVPEDDPRFSDVQDISDVPSHLLKEIEHFFSVYKNLEGKVVETKGWEGKESAK
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHH
KELLRSLEMYKEKYE
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDRVLYSPVVYPADYGFIPKSTYDDGDPMDILVLMEQPTFPGCLIEARPIGIMGMIDGG
CCCCHHCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCEEEEEECCC
DKDYKILAVPEDDPRFSDVQDISDVPSHLLKEIEHFFSVYKNLEGKVVETKGWEGKESAK
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHH
KELLRSLEMYKEKYE
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]