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The map label for this gene is rpoE1 [H]

Identifier: 148642257

GI number: 148642257

Start: 190086

End: 190643

Strand: Reverse

Name: rpoE1 [H]

Synonym: Msm_0197

Alternate gene names: 148642257

Gene position: 190643-190086 (Counterclockwise)

Preceding gene: 148642258

Following gene: 148642256

Centisome position: 10.29

GC content: 31.54

Gene sequence:

>558_bases
ATGACTAAAATTGAGGATACTGTAAGAATACCACCTTATAGATTTGATGATCCTCTTGAAGAGGTAGCTATTCAAACTTT
AAATGATACATATAACGGCCGTTTAGATAAAAAATTAGGTTTACTTATTTGTGTTAATGAAATTGAAGAAATTGGTGAAG
GTAGACTCATCATGGGAGATGGAGCTTCTTACCATAATGTTGTGTTTAATGCAATATTCTTCAAACCTGAACAGCAAGAA
GTTATTGATGGTGAAGTAATAGAAATAGCTGAATTTGGTGCTTTTGTCAGAATTGGACCTATGGACGGTTTGGTTCATGT
TTCCCAAGTCACTGATGATTATATTAATTACGATTCTAAAAGAGGAGCACTCCTTGCAAAAGAATCTAATAAAACTTTAG
ATGAAGGAGATTTAGTTAGAACAAGAATTGTAGCTATCAGTCTTAAAGATAACTCTACCAAAAATACTAAAATAGGTCTT
ACCATGAGACAAAATAATTTAGGTAGATTTGAATGGATTGAAGAAGCTAAAAATAAAAATAAGAAGGCTAAATCATGA

Upstream 100 bases:

>100_bases
ACTAAAGGCTGGGAAGGTAAAGAGTCAGCTAAAAAAGAACTTTTAAGATCTTTAGAAATGTATAAAGAAAAATACGAATA
GAGGGATTGTTTTGTATTAC

Downstream 100 bases:

>100_bases
ATGCATGTACAGTTTGTAAATATATTACTAATAAAGAACGTTGTCCAGTCTGCGGTAACCCAACTTCAGATAACTGGAGC
GGTCTTTTAATAATTACAAA

Product: DNA-directed RNA polymerase subunit E'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTKIEDTVRIPPYRFDDPLEEVAIQTLNDTYNGRLDKKLGLLICVNEIEEIGEGRLIMGDGASYHNVVFNAIFFKPEQQE
VIDGEVIEIAEFGAFVRIGPMDGLVHVSQVTDDYINYDSKRGALLAKESNKTLDEGDLVRTRIVAISLKDNSTKNTKIGL
TMRQNNLGRFEWIEEAKNKNKKAKS

Sequences:

>Translated_185_residues
MTKIEDTVRIPPYRFDDPLEEVAIQTLNDTYNGRLDKKLGLLICVNEIEEIGEGRLIMGDGASYHNVVFNAIFFKPEQQE
VIDGEVIEIAEFGAFVRIGPMDGLVHVSQVTDDYINYDSKRGALLAKESNKTLDEGDLVRTRIVAISLKDNSTKNTKIGL
TMRQNNLGRFEWIEEAKNKNKKAKS
>Mature_184_residues
TKIEDTVRIPPYRFDDPLEEVAIQTLNDTYNGRLDKKLGLLICVNEIEEIGEGRLIMGDGASYHNVVFNAIFFKPEQQEV
IDGEVIEIAEFGAFVRIGPMDGLVHVSQVTDDYINYDSKRGALLAKESNKTLDEGDLVRTRIVAISLKDNSTKNTKIGLT
MRQNNLGRFEWIEEAKNKNKKAKS

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG1095

COG function: function code K; DNA-directed RNA polymerase, subunit E'

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI24475814, Length=158, Percent_Identity=25.3164556962025, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI65301161, Length=158, Percent_Identity=25.3164556962025, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17566938, Length=193, Percent_Identity=27.979274611399, Blast_Score=63, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322705, Length=165, Percent_Identity=27.8787878787879, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR005576
- InterPro:   IPR004519 [H]

Pfam domain/function: PF03876 RNA_pol_Rpb7_N; PF00575 S1 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 20893; Mature: 20762

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIEDTVRIPPYRFDDPLEEVAIQTLNDTYNGRLDKKLGLLICVNEIEEIGEGRLIMGD
CCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCEECCCCEEEEEEHHHHHHCCCCEEEEC
GASYHNVVFNAIFFKPEQQEVIDGEVIEIAEFGAFVRIGPMDGLVHVSQVTDDYINYDSK
CCCHHHHEEEHEEECCCHHHHCCCCEEEEECCCCEEEECCCCCEEEHHHHHHHHHCCCCC
RGALLAKESNKTLDEGDLVRTRIVAISLKDNSTKNTKIGLTMRQNNLGRFEWIEEAKNKN
CCEEEEECCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHCCC
KKAKS
CCCCC
>Mature Secondary Structure 
TKIEDTVRIPPYRFDDPLEEVAIQTLNDTYNGRLDKKLGLLICVNEIEEIGEGRLIMGD
CCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCEECCCCEEEEEEHHHHHHCCCCEEEEC
GASYHNVVFNAIFFKPEQQEVIDGEVIEIAEFGAFVRIGPMDGLVHVSQVTDDYINYDSK
CCCHHHHEEEHEEECCCHHHHCCCCEEEEECCCCEEEECCCCCEEEHHHHHHHHHCCCCC
RGALLAKESNKTLDEGDLVRTRIVAISLKDNSTKNTKIGLTMRQNNLGRFEWIEEAKNKN
CCEEEEECCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHCCC
KKAKS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]