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The map label for this gene is 148642218

Identifier: 148642218

GI number: 148642218

Start: 149795

End: 150394

Strand: Reverse

Name: 148642218

Synonym: Msm_0158

Alternate gene names: NA

Gene position: 150394-149795 (Counterclockwise)

Preceding gene: 148642220

Following gene: 148642217

Centisome position: 8.12

GC content: 26.5

Gene sequence:

>600_bases
ATGGATAGTGATTATATTTCTATTGTTAAAGATGGATCTGGTGTTGGAAATATCAGTTTTCATGAAAAAAACACATCTGT
TGTTAATACATCACAATATATTTTGCCTAAGGAAAATCTAAATATTCATTTTATTTTCTATTTACTTCAAACAATTAATT
TAAATAAATATAAAACAGGGTCAACAATCCCCCACATATATTTTAAAGATTATTCAATTGAAAAAGTCAAAATACCTAAA
TATGATGAACAGAAAAAAATAGGAATATTGCTTAAGAATCTAGATGCAAAAATAGAAATTTTAGATAATAAACTACAAAT
GTGCCAGAATTTCAAGAAGTATTTAATGCAGCAGATTTTCACACAGAAATTAAGATTTACAGATTACATAGAGGAGTGGA
AAACTATAAAACTAAAAGATGTTGGCGAAATAAATACTGGAAACACTCCTTCAACTAAAGTTAATGAATATTATTCTCCT
AAAAAATATTTATGGGTAACTCCATCAGATATATCCTCAAAATACATTTTTGACACAGCGAAAAGATTATCTGACGAAGG
TGCTAAAAAAGGACGCTTTGTTAAAAAAATAGTGTATTAG

Upstream 100 bases:

>100_bases
TAAATCATCTAGCATATCTATTAATCAATTGGATGATAATGTTGGCAATTATCCTTTATATGGCGCTAGTGGATTTTTAA
AATGCATAGATTTTTATGAA

Downstream 100 bases:

>100_bases
TCACATGCATAGCCAGTATTGGAAAAAATTGTATTATTCTAGAAGATGGATATTTTAATCAACAAATTAATTCTATAACT
CCATCACAAAGTTTTGATTT

Product: type I restriction-modification system methylase, subunit S

Products: NA

Alternate protein names: S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MDSDYISIVKDGSGVGNISFHEKNTSVVNTSQYILPKENLNIHFIFYLLQTINLNKYKTGSTIPHIYFKDYSIEKVKIPK
YDEQKKIGILLKNLDAKIEILDNKLQMCQNFKKYLMQQIFTQKLRFTDYIEEWKTIKLKDVGEINTGNTPSTKVNEYYSP
KKYLWVTPSDISSKYIFDTAKRLSDEGAKKGRFVKKIVY

Sequences:

>Translated_199_residues
MDSDYISIVKDGSGVGNISFHEKNTSVVNTSQYILPKENLNIHFIFYLLQTINLNKYKTGSTIPHIYFKDYSIEKVKIPK
YDEQKKIGILLKNLDAKIEILDNKLQMCQNFKKYLMQQIFTQKLRFTDYIEEWKTIKLKDVGEINTGNTPSTKVNEYYSP
KKYLWVTPSDISSKYIFDTAKRLSDEGAKKGRFVKKIVY
>Mature_199_residues
MDSDYISIVKDGSGVGNISFHEKNTSVVNTSQYILPKENLNIHFIFYLLQTINLNKYKTGSTIPHIYFKDYSIEKVKIPK
YDEQKKIGILLKNLDAKIEILDNKLQMCQNFKKYLMQQIFTQKLRFTDYIEEWKTIKLKDVGEINTGNTPSTKVNEYYSP
KKYLWVTPSDISSKYIFDTAKRLSDEGAKKGRFVKKIVY

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 23295; Mature: 23295

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSDYISIVKDGSGVGNISFHEKNTSVVNTSQYILPKENLNIHFIFYLLQTINLNKYKTG
CCCCEEEEEECCCCCCCEEEECCCCCEEECCEEEEECCCCCEEEHHHHHHHCCCCCEECC
STIPHIYFKDYSIEKVKIPKYDEQKKIGILLKNLDAKIEILDNKLQMCQNFKKYLMQQIF
CCCCEEEEECCCEEEEECCCCCCCHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHH
TQKLRFTDYIEEWKTIKLKDVGEINTGNTPSTKVNEYYSPKKYLWVTPSDISSKYIFDTA
HHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHH
KRLSDEGAKKGRFVKKIVY
HHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MDSDYISIVKDGSGVGNISFHEKNTSVVNTSQYILPKENLNIHFIFYLLQTINLNKYKTG
CCCCEEEEEECCCCCCCEEEECCCCCEEECCEEEEECCCCCEEEHHHHHHHCCCCCEECC
STIPHIYFKDYSIEKVKIPKYDEQKKIGILLKNLDAKIEILDNKLQMCQNFKKYLMQQIF
CCCCEEEEECCCEEEEECCCCCCCHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHH
TQKLRFTDYIEEWKTIKLKDVGEINTGNTPSTKVNEYYSPKKYLWVTPSDISSKYIFDTA
HHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHH
KRLSDEGAKKGRFVKKIVY
HHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]