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The map label for this gene is asnB [C]

Identifier: 148642220

GI number: 148642220

Start: 150953

End: 152380

Strand: Reverse

Name: asnB [C]

Synonym: Msm_0160

Alternate gene names: 148642220

Gene position: 152380-150953 (Counterclockwise)

Preceding gene: 148642221

Following gene: 148642218

Centisome position: 8.22

GC content: 34.52

Gene sequence:

>1428_bases
ATGTGTTCAATAGTTGGTCTTCAAGGTAATTTCAAAGCTGATGATTTAATAGCTATGTTAAAAACATCAAAAAATAGGGG
AAAAGATGCTTCAGGAATATTTTTAGATGGTAAAATACAAAAAGGTATTGATTTAAATGATTTCAATGATAAAAACGCTT
ATTATTTAGGTTTAGGCCATAATCTGCTGGCTGTCCATAATTTGGAAAACAGAACTTCCCATACTCAGCCGATAGCTAAA
AACACTCTGACTCTGGTTTTCAATGGTGAGATTTATAATTATTCTTCCCTAAAGAATTTGCTGGCTAAACTGACAAAAAA
TGACAATATAAATTCTGATTCTGAGTTATTAATTAATCTGATTGATTTTTATTATAAGGATGACCTTTTAAGAGCAGTAA
CTCAGGTCAATAATCTTTTGGATGGGGATTATGCTTATGCTGTTACAGATGGCATTAACTTGGCTGTTTCAAGAGATCCT
TTAGGTGTCAAACCTTTATTTTACACACATAATGCTTTTGCTTCTTCAAAACAGTGCTTGAAAGGAGATATCAAAACATT
AAAGCCGGGACATATACTTTACAACTGGGAAGAGATACCTCCTAAAACGGATATTTTCCAGAAGAGGTTTGAAACCGACA
ACAGAAAACTTGAAAAGCTTCTTAAACTGGCTGTTTTAAAAAGGGTTGAGGATTTGAATGAAGTTGGAGTAATATTTTCA
GGAGGTGTTGACAGCTCTTTGATAGCTTTGCTGCTTCGGGAAATATCTTTAAATAAAAATCTTAAAGTTACTTTGTATGC
TGTCGGTAAAAAGGATTCCAAGGATGTGGTTGCAGCTAAGTGTGTAGCCGAGTATTTGGGTCTTCCTCTAAAGGTTCATG
ACATTACGGAAAGTATTGTAAAGGACAGTCTTAAAGATGTTGTTCAGGCAATTGGTGAAAACAATCTGATGAAGATTGGA
GTGGGAATGACTGTTTATCTTGCTTCTAAAATGATAGTTGAAGATAATATTAAGGTAGCTATTTCCGGTCAGGGTGCTGA
TGAGCTGTTTGGGGGCTATAACAGATATCTGAATAGCTATATGGAAAATACTTTAGATGATGAATTAAGATATGATATGG
CTAATATGTATCATGTTAATCTGGAACGTGATGATGCATGCTCAATGGCAAATGGCGTTGAGCTGCGTCTTCCTTTTTTA
GATAAGAATCTTGTTGAATTTGCCCTCAATATTCCGGTCAGATATAAGATTTCAGGTTCTGATGATAAGCTGCGCAAAAA
TATTTTAAGAAAAACGGCTTTCAATTTGGGACTGGATAAGCAGATTGCTTACAGACCTAAAAAGGCAGCCCAGTACGGCA
CGGGTATTGACAAAATTTTAAGAAAAAAAGTTTTAAAGGATACTGATATTGAGGAGTATTTAAAATAG

Upstream 100 bases:

>100_bases
GGATGTAATCAGTTAAAATAATGCACTATGAATTGAAATAATCAAATGTTTACTTTGTACTATCATTATTTTTTTAAACT
CCAAAATTTAATATTAAATC

Downstream 100 bases:

>100_bases
AGTTTAAAACTCTATTTAAGGACAGTTACTCCATACCCCATATCTAATCCGGTTACCGGTTCAATAGCTGTTATGATATA
TTTTCCAGGTATCAGATTTA

Product: asparagine synthetase, AsnB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MCSIVGLQGNFKADDLIAMLKTSKNRGKDASGIFLDGKIQKGIDLNDFNDKNAYYLGLGHNLLAVHNLENRTSHTQPIAK
NTLTLVFNGEIYNYSSLKNLLAKLTKNDNINSDSELLINLIDFYYKDDLLRAVTQVNNLLDGDYAYAVTDGINLAVSRDP
LGVKPLFYTHNAFASSKQCLKGDIKTLKPGHILYNWEEIPPKTDIFQKRFETDNRKLEKLLKLAVLKRVEDLNEVGVIFS
GGVDSSLIALLLREISLNKNLKVTLYAVGKKDSKDVVAAKCVAEYLGLPLKVHDITESIVKDSLKDVVQAIGENNLMKIG
VGMTVYLASKMIVEDNIKVAISGQGADELFGGYNRYLNSYMENTLDDELRYDMANMYHVNLERDDACSMANGVELRLPFL
DKNLVEFALNIPVRYKISGSDDKLRKNILRKTAFNLGLDKQIAYRPKKAAQYGTGIDKILRKKVLKDTDIEEYLK

Sequences:

>Translated_475_residues
MCSIVGLQGNFKADDLIAMLKTSKNRGKDASGIFLDGKIQKGIDLNDFNDKNAYYLGLGHNLLAVHNLENRTSHTQPIAK
NTLTLVFNGEIYNYSSLKNLLAKLTKNDNINSDSELLINLIDFYYKDDLLRAVTQVNNLLDGDYAYAVTDGINLAVSRDP
LGVKPLFYTHNAFASSKQCLKGDIKTLKPGHILYNWEEIPPKTDIFQKRFETDNRKLEKLLKLAVLKRVEDLNEVGVIFS
GGVDSSLIALLLREISLNKNLKVTLYAVGKKDSKDVVAAKCVAEYLGLPLKVHDITESIVKDSLKDVVQAIGENNLMKIG
VGMTVYLASKMIVEDNIKVAISGQGADELFGGYNRYLNSYMENTLDDELRYDMANMYHVNLERDDACSMANGVELRLPFL
DKNLVEFALNIPVRYKISGSDDKLRKNILRKTAFNLGLDKQIAYRPKKAAQYGTGIDKILRKKVLKDTDIEEYLK
>Mature_475_residues
MCSIVGLQGNFKADDLIAMLKTSKNRGKDASGIFLDGKIQKGIDLNDFNDKNAYYLGLGHNLLAVHNLENRTSHTQPIAK
NTLTLVFNGEIYNYSSLKNLLAKLTKNDNINSDSELLINLIDFYYKDDLLRAVTQVNNLLDGDYAYAVTDGINLAVSRDP
LGVKPLFYTHNAFASSKQCLKGDIKTLKPGHILYNWEEIPPKTDIFQKRFETDNRKLEKLLKLAVLKRVEDLNEVGVIFS
GGVDSSLIALLLREISLNKNLKVTLYAVGKKDSKDVVAAKCVAEYLGLPLKVHDITESIVKDSLKDVVQAIGENNLMKIG
VGMTVYLASKMIVEDNIKVAISGQGADELFGGYNRYLNSYMENTLDDELRYDMANMYHVNLERDDACSMANGVELRLPFL
DKNLVEFALNIPVRYKISGSDDKLRKNILRKTAFNLGLDKQIAYRPKKAAQYGTGIDKILRKKVLKDTDIEEYLK

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=516, Percent_Identity=26.7441860465116, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI168229252, Length=516, Percent_Identity=26.7441860465116, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI168229250, Length=516, Percent_Identity=26.7441860465116, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI296010848, Length=477, Percent_Identity=27.2536687631027, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI296010852, Length=400, Percent_Identity=27.25, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI296010850, Length=400, Percent_Identity=27.25, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI9506697, Length=313, Percent_Identity=27.1565495207668, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786889, Length=502, Percent_Identity=25.8964143426295, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560178, Length=425, Percent_Identity=30.3529411764706, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71993933, Length=511, Percent_Identity=28.5714285714286, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI25147557, Length=511, Percent_Identity=28.5714285714286, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25147560, Length=297, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6325403, Length=539, Percent_Identity=26.1595547309833, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321563, Length=460, Percent_Identity=27.6086956521739, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323542, Length=468, Percent_Identity=26.0683760683761, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI45553209, Length=475, Percent_Identity=27.5789473684211, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI221329991, Length=268, Percent_Identity=28.3582089552239, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 53281; Mature: 53281

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIVGLQGNFKADDLIAMLKTSKNRGKDASGIFLDGKIQKGIDLNDFNDKNAYYLGLGH
CCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECC
NLLAVHNLENRTSHTQPIAKNTLTLVFNGEIYNYSSLKNLLAKLTKNDNINSDSELLINL
CEEEEECCCCCCCCCCCCCCCEEEEEECCCEECHHHHHHHHHHHHCCCCCCCHHHHHHHH
IDFYYKDDLLRAVTQVNNLLDGDYAYAVTDGINLAVSRDPLGVKPLFYTHNAFASSKQCL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCEEEECCHHHCCHHHH
KGDIKTLKPGHILYNWEEIPPKTDIFQKRFETDNRKLEKLLKLAVLKRVEDLNEVGVIFS
HCCHHHCCCCEEEECHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
GGVDSSLIALLLREISLNKNLKVTLYAVGKKDSKDVVAAKCVAEYLGLPLKVHDITESIV
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHH
KDSLKDVVQAIGENNLMKIGVGMTVYLASKMIVEDNIKVAISGQGADELFGGYNRYLNSY
HHHHHHHHHHHCCCCEEEEECCEEEEHHHHHEEECCEEEEEECCCHHHHHHHHHHHHHHH
MENTLDDELRYDMANMYHVNLERDDACSMANGVELRLPFLDKNLVEFALNIPVRYKISGS
HHHHHHHHHHHHHHHEEEEECCCCCHHHHCCCCEEEECCCCHHHHHHHCCCCEEEEECCC
DDKLRKNILRKTAFNLGLDKQIAYRPKKAAQYGTGIDKILRKKVLKDTDIEEYLK
HHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHC
>Mature Secondary Structure
MCSIVGLQGNFKADDLIAMLKTSKNRGKDASGIFLDGKIQKGIDLNDFNDKNAYYLGLGH
CCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECC
NLLAVHNLENRTSHTQPIAKNTLTLVFNGEIYNYSSLKNLLAKLTKNDNINSDSELLINL
CEEEEECCCCCCCCCCCCCCCEEEEEECCCEECHHHHHHHHHHHHCCCCCCCHHHHHHHH
IDFYYKDDLLRAVTQVNNLLDGDYAYAVTDGINLAVSRDPLGVKPLFYTHNAFASSKQCL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCEEEECCHHHCCHHHH
KGDIKTLKPGHILYNWEEIPPKTDIFQKRFETDNRKLEKLLKLAVLKRVEDLNEVGVIFS
HCCHHHCCCCEEEECHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
GGVDSSLIALLLREISLNKNLKVTLYAVGKKDSKDVVAAKCVAEYLGLPLKVHDITESIV
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHH
KDSLKDVVQAIGENNLMKIGVGMTVYLASKMIVEDNIKVAISGQGADELFGGYNRYLNSY
HHHHHHHHHHHCCCCEEEEECCEEEEHHHHHEEECCEEEEEECCCHHHHHHHHHHHHHHH
MENTLDDELRYDMANMYHVNLERDDACSMANGVELRLPFLDKNLVEFALNIPVRYKISGS
HHHHHHHHHHHHHHHEEEEECCCCCHHHHCCCCEEEECCCCHHHHHHHCCCCEEEEECCC
DDKLRKNILRKTAFNLGLDKQIAYRPKKAAQYGTGIDKILRKKVLKDTDIEEYLK
HHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]