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The map label for this gene is hypA

Identifier: 148642168

GI number: 148642168

Start: 102569

End: 102946

Strand: Reverse

Name: hypA

Synonym: Msm_0108

Alternate gene names: 148642168

Gene position: 102946-102569 (Counterclockwise)

Preceding gene: 148642169

Following gene: 148642167

Centisome position: 5.56

GC content: 32.28

Gene sequence:

>378_bases
ATGCATGAATTATCTATGGCTCAAGGAATAATAAATGCAGTAATAGATACTGCAGAAAGTAACAATGCAACTGAAGTTAC
TGAAGTTGGTATTGAAATTGGCAGATTGGCTATGATAAACCCTGAACAGTTACGTTTCATGTTAAGCGTTTTAGTTGAAA
ATACCATAGTTGAAGATGCCGATATTAAAATTGAAGAGATACCTGTTGAAATTAATTGTCCTGAATGCGGATTTAAAGGA
GTAGCAGAACTGGATGATAAAGATCACTATGCTCCAATTGTAGAATGTCCGAAATGTGGAAATAAAAGAATATCTATTTT
AAACGGAAAAGATTGTGTTGTTAAAAATATTGTTATTGAGAAACCTGATGATGATTAG

Upstream 100 bases:

>100_bases
TAAATTTTGCTGATTTTCACATTAATTAACAATATTTATTAATCATCCTAACTATAATTATAATATTAAGATTTTTTTCT
GAATATGTAGGAGAAATAAA

Downstream 100 bases:

>100_bases
ATAGGAGGAATTGATTATGCATAATGTAGCTGATGTAGAAGTACAAAAAAATATCATGGATGCTAATAAAAGATTAGCAA
ATAGAAATAAAGAAAATCTT

Product: hydrogenase nickel incorporation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MHELSMAQGIINAVIDTAESNNATEVTEVGIEIGRLAMINPEQLRFMLSVLVENTIVEDADIKIEEIPVEINCPECGFKG
VAELDDKDHYAPIVECPKCGNKRISILNGKDCVVKNIVIEKPDDD

Sequences:

>Translated_125_residues
MHELSMAQGIINAVIDTAESNNATEVTEVGIEIGRLAMINPEQLRFMLSVLVENTIVEDADIKIEEIPVEINCPECGFKG
VAELDDKDHYAPIVECPKCGNKRISILNGKDCVVKNIVIEKPDDD
>Mature_125_residues
MHELSMAQGIINAVIDTAESNNATEVTEVGIEIGRLAMINPEQLRFMLSVLVENTIVEDADIKIEEIPVEINCPECGFKG
VAELDDKDHYAPIVECPKCGNKRISILNGKDCVVKNIVIEKPDDD

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family

Homologues:

Organism=Escherichia coli, GI1789365, Length=120, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPA_METS3 (A5UJD5)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001272681.1
- STRING:   A5UJD5
- GeneID:   5216291
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0108
- eggNOG:   arNOG11688
- HOGENOM:   HBG688671
- OMA:   MHELSYA
- ProtClustDB:   PRK00762
- HAMAP:   MF_00213
- InterPro:   IPR000688
- InterPro:   IPR020538
- TIGRFAMs:   TIGR00100

Pfam domain/function: PF01155 HypA

EC number: NA

Molecular weight: Translated: 13751; Mature: 13751

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELSMAQGIINAVIDTAESNNATEVTEVGIEIGRLAMINPEQLRFMLSVLVENTIVEDA
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHCEEEEECHHHHHHHHHHHHHHHCCCCC
DIKIEEIPVEINCPECGFKGVAELDDKDHYAPIVECPKCGNKRISILNGKDCVVKNIVIE
CCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCEEEEEEEEE
KPDDD
CCCCC
>Mature Secondary Structure
MHELSMAQGIINAVIDTAESNNATEVTEVGIEIGRLAMINPEQLRFMLSVLVENTIVEDA
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHCEEEEECHHHHHHHHHHHHHHHCCCCC
DIKIEEIPVEINCPECGFKGVAELDDKDHYAPIVECPKCGNKRISILNGKDCVVKNIVIE
CCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCEEEEEEEEE
KPDDD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA