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The map label for this gene is cofH [H]

Identifier: 148642139

GI number: 148642139

Start: 77576

End: 78727

Strand: Reverse

Name: cofH [H]

Synonym: Msm_0079

Alternate gene names: 148642139

Gene position: 78727-77576 (Counterclockwise)

Preceding gene: 148642142

Following gene: 148642138

Centisome position: 4.25

GC content: 34.98

Gene sequence:

>1152_bases
ATGTTTGATAAACTACCTATATCTTCTAAAACAGAAAAAATTTTAACTAAATCTCTTGATGAACCAATTTCTGCTGAAGA
TGCAAACTATTTAATAAATATTAAAGGGTCTGACTTGTATCCCTTACTTGCAACTGCTGATTATCTCAGGCAAGAAATTG
CTGGAAACAACGTTACATTTATTAATAATTGTAATATTAACTTCACAAATATTTGTACTGTAAGATGTGGTTTTTGTGCA
TTTGGTAAGGATGCAGATGACCCTGAAGCATATATTTTAGATGATGAAGCTATTTTAAAAAAAGCTCAGGGGGCTGTTGA
CAAAGGAGCTCATGAGTTTTGTGTCATGGGGGGAGTTTTACCTGATGCAGATGTTGAATATTATGAACATTTGCTTCAAT
TGTTAAAAGGGGAATTTCCCAATATTATGATTCATGGTTTTTCACCAACTATGATTAAAGATGCCAGTGTTGTATCAGGC
ATAAGTGTTGAAGATGCATTTGAAAGGCTTAAAAGTGCGGGTTTAGATACATTGCCTGGAACTGCTGCAGAAATATTGAC
TGACAGATCCAGAGAGATAATCTGTCCGGAAAAGGTAACAACACAGGAATGGATTGATATTGTAAAAACAGCTCATGAAG
TTGGAATTCCAGGCTCAGCTACTATTATGTACGGGCATGTGGAAACTCCTGAAGAACGTGTAGAACACATTGATATTATC
AGAAAAATTCAGCAGGAAACTCACGGTTTTACAGAATTTATTCCGATGACATTTATGCATGAGTATTCTCCAATTTTTTT
AGAAGGTCAGCAAAACTTGGGAGCTACAGGTACTGAGGATTTAAAATTATATGCAGTAGCTAGATTAATGCTTAAGGATT
TGATTCCAAATATCCAAGTATCCTGGGTAAAAATGGGTTTCAGATTTGCACAGGTATCTTTAACAGCTGGTGCAAATGAT
TTGGGTGGAACTTTAGGTGGAGATGAATTGTCTGAAGCTTCAGGAGCACCTGATGGAGTTGAGGCATCTATTGATACTTT
AAGTAATATGGTTAGAGATTTAGGTAGAAACCCTATTGAGAGGAATTCAAAATACACAGAATTTTATCCGATTGAATCTA
AAAGTTCTGATGTGAAAATAGTATCAAAATAA

Upstream 100 bases:

>100_bases
TAAAATATTGAATGAATTTACTATGTTTTCATTTTGTTAACTTTGCAGTGATTCAATTATATGGAAAGATATTTATTTAA
TTAAATTATTATATTATTTT

Downstream 100 bases:

>100_bases
AGTTTTAAAGATGATAACATGAATATGGCAGATATAGGTTTATTAGTTTTAGTGTATATTTATGTTATCGCAATTTTTGT
TGTTTCAGAGAAGTTTTTAA

Product: CofH protein (7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO)/F420 biosynthesis)

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MFDKLPISSKTEKILTKSLDEPISAEDANYLINIKGSDLYPLLATADYLRQEIAGNNVTFINNCNINFTNICTVRCGFCA
FGKDADDPEAYILDDEAILKKAQGAVDKGAHEFCVMGGVLPDADVEYYEHLLQLLKGEFPNIMIHGFSPTMIKDASVVSG
ISVEDAFERLKSAGLDTLPGTAAEILTDRSREIICPEKVTTQEWIDIVKTAHEVGIPGSATIMYGHVETPEERVEHIDII
RKIQQETHGFTEFIPMTFMHEYSPIFLEGQQNLGATGTEDLKLYAVARLMLKDLIPNIQVSWVKMGFRFAQVSLTAGAND
LGGTLGGDELSEASGAPDGVEASIDTLSNMVRDLGRNPIERNSKYTEFYPIESKSSDVKIVSK

Sequences:

>Translated_383_residues
MFDKLPISSKTEKILTKSLDEPISAEDANYLINIKGSDLYPLLATADYLRQEIAGNNVTFINNCNINFTNICTVRCGFCA
FGKDADDPEAYILDDEAILKKAQGAVDKGAHEFCVMGGVLPDADVEYYEHLLQLLKGEFPNIMIHGFSPTMIKDASVVSG
ISVEDAFERLKSAGLDTLPGTAAEILTDRSREIICPEKVTTQEWIDIVKTAHEVGIPGSATIMYGHVETPEERVEHIDII
RKIQQETHGFTEFIPMTFMHEYSPIFLEGQQNLGATGTEDLKLYAVARLMLKDLIPNIQVSWVKMGFRFAQVSLTAGAND
LGGTLGGDELSEASGAPDGVEASIDTLSNMVRDLGRNPIERNSKYTEFYPIESKSSDVKIVSK
>Mature_383_residues
MFDKLPISSKTEKILTKSLDEPISAEDANYLINIKGSDLYPLLATADYLRQEIAGNNVTFINNCNINFTNICTVRCGFCA
FGKDADDPEAYILDDEAILKKAQGAVDKGAHEFCVMGGVLPDADVEYYEHLLQLLKGEFPNIMIHGFSPTMIKDASVVSG
ISVEDAFERLKSAGLDTLPGTAAEILTDRSREIICPEKVTTQEWIDIVKTAHEVGIPGSATIMYGHVETPEERVEHIDII
RKIQQETHGFTEFIPMTFMHEYSPIFLEGQQNLGATGTEDLKLYAVARLMLKDLIPNIQVSWVKMGFRFAQVSLTAGAND
LGGTLGGDELSEASGAPDGVEASIDTLSNMVRDLGRNPIERNSKYTEFYPIESKSSDVKIVSK

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42141; Mature: 42141

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKLPISSKTEKILTKSLDEPISAEDANYLINIKGSDLYPLLATADYLRQEIAGNNVTF
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEE
INNCNINFTNICTVRCGFCAFGKDADDPEAYILDDEAILKKAQGAVDKGAHEFCVMGGVL
EECCCCCEEEEEEEEECEEECCCCCCCCCEEEECHHHHHHHHHCCCCCCCHHHHHCCCCC
PDADVEYYEHLLQLLKGEFPNIMIHGFSPTMIKDASVVSGISVEDAFERLKSAGLDTLPG
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEECCHHHCCCCHHHHHHHHHHCCCCCCCC
TAAEILTDRSREIICPEKVTTQEWIDIVKTAHEVGIPGSATIMYGHVETPEERVEHIDII
CHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
RKIQQETHGFTEFIPMTFMHEYSPIFLEGQQNLGATGTEDLKLYAVARLMLKDLIPNIQV
HHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
SWVKMGFRFAQVSLTAGANDLGGTLGGDELSEASGAPDGVEASIDTLSNMVRDLGRNPIE
EEHHHCEEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
RNSKYTEFYPIESKSSDVKIVSK
CCCCCCEEEECCCCCCCEEEECC
>Mature Secondary Structure
MFDKLPISSKTEKILTKSLDEPISAEDANYLINIKGSDLYPLLATADYLRQEIAGNNVTF
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEE
INNCNINFTNICTVRCGFCAFGKDADDPEAYILDDEAILKKAQGAVDKGAHEFCVMGGVL
EECCCCCEEEEEEEEECEEECCCCCCCCCEEEECHHHHHHHHHCCCCCCCHHHHHCCCCC
PDADVEYYEHLLQLLKGEFPNIMIHGFSPTMIKDASVVSGISVEDAFERLKSAGLDTLPG
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEECCHHHCCCCHHHHHHHHHHCCCCCCCC
TAAEILTDRSREIICPEKVTTQEWIDIVKTAHEVGIPGSATIMYGHVETPEERVEHIDII
CHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHH
RKIQQETHGFTEFIPMTFMHEYSPIFLEGQQNLGATGTEDLKLYAVARLMLKDLIPNIQV
HHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
SWVKMGFRFAQVSLTAGANDLGGTLGGDELSEASGAPDGVEASIDTLSNMVRDLGRNPIE
EEHHHCEEEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
RNSKYTEFYPIESKSSDVKIVSK
CCCCCCEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]