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The map label for this gene is gltB [C]

Identifier: 148642087

GI number: 148642087

Start: 19640

End: 21277

Strand: Reverse

Name: gltB [C]

Synonym: Msm_0027

Alternate gene names: 148642087

Gene position: 21277-19640 (Counterclockwise)

Preceding gene: 148642089

Following gene: 148642086

Centisome position: 1.15

GC content: 34.55

Gene sequence:

>1638_bases
ATGAAATACCAGTGCCAGATATGCGGTTATATTCATGATGAAGAAAAAGAAGGCAAATCCTTTAAAGAACTTGAAAAATG
TCCTGATTGCAAATCAGATGTTTCTAATTTTGAAATAATGAAAAAAGATATGGCCTATAAGACATATGAATGTGAAGTTT
GTCATTATGTTTATGATGAAGAAAAAGAGGGTCAGGTACTTGTTAAACTTGGAAAATGCCCGGTATGCGGTTCGCCGCTA
TCAAAATTCAAAGCAATCCAAAAAACAGAATATAGAATATATCAGTGTAGGATTTGTAATTATATTCATGATGAAAAAAC
TGAAAAAATACCATTAAAAGACCTTAAAGAATGTCCGGATTGCGGATCAGATATATCTAACTTTGAACCAGCTGAAACAA
ATGAAAACAATTGGTTAATTTCATTTCCGAAACAGTACATCAGAAATGATGAATCAGTAAGACATATGGACACCATTTAT
AAGTTATGCGATTCCGGAAAACCCATTACAGCACCAATGGCTACAGAATTGCCAATGCCTGATTGGGATGATATTCTTAT
TTTAGGAAACCAGTTAAATCCTATGCCTTTAGAAGAAGATGCAGAAGTGTCAGCTACTACGGTAATTGGTAAAAATGCTG
AAAAACCTTTAGTTATTGAAAATCCTGTATATGTATCCCACATGTCTTACGGGGCCTTGTCTAAGGAAAGTAAAATTGCT
TTGGCTAAAGGAAGCTTTAAGGCTAAAACAGCAATGTGTAGTGGAGAGGGAGGAATACTGCCGGAAGTTAAAAATGAGGC
ATACAAATACATTTTTGAATATGTTCCGAACAAGTATTCAGTAACTGATGAAAACTTAAAAACTTCAGATGCAATTGAAA
TTAAAATAGGTCAGGCAACAAAACCTGGAATGGGTGGATTATTGCCTGGTGATAAAGTAACGCCTGAAATAGCCAAAGTA
AGAGGTAAAAAAGCAGGTGAAGACATAATATCCCCATCAAGATTCCCGAATATTAATTCTAAGAAAGATTTAAAAGATCT
GGTAGATGAATTAAGATTAAAATCTGAAGGCCGTCCGATAGGTATTAAAATAGCTGCAGGATATATTGAAAATGATTTGG
AATTTATTAGTTATGCCAAACCTGATTTCATTACAGTTGACGGAAGAGGGGGAGCTACTGGAGCCAGCCCATTATTAGTT
AGGGATTCAACAAGTATTCCAACAATATTTGCATTACACAGAGCCAGAAAATATCTGGATGAACATGATTTGGATATTGA
TCTGGTAATTACTGGTGGTTTAAGAGTTTCCAGTGACTTTGCAAAAGCATTAGCTATGGGAGCAGATGCAATAGCTATAG
CTTCAGCCAGTTTAATTGCAGTGGCATGTCAGCAGTACAGATTATGTGATAAAGGGCAATGTCCTGTGGGAGTTGCAACA
CAGGATAAAGAACTGCGTTCCAGATTAAAAACAGATATTGGAGCAAAAAGATTAACCAATTATCTTAATGCAAGTCTTGA
AGAAATTAAAACTTTTGCAAGAATAACAGGACACAGTGATATTCATGATTTGAATGTTTCAAATTTAGCTACATTCAATA
GTGAAATATCTGATTATACAAATATTAAACATGTTTAG

Upstream 100 bases:

>100_bases
AACTTTTACTTTTTTTAACATTGAATTAATTATATTATACAAAAATATTTATATTATTGTTAAAGTTATATAATATATTA
ATTATGGTGGAGTTGGTAAA

Downstream 100 bases:

>100_bases
AGGTTATCAGTTTAAAAACCTCATTTCTGTTTATTAAAAAACGTCTGGCGATAATTGCATTGCTTAAAATGTCGCAGAGC
TTTCTTTTTTTACACAGTTT

Product: glutamate synthase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MKYQCQICGYIHDEEKEGKSFKELEKCPDCKSDVSNFEIMKKDMAYKTYECEVCHYVYDEEKEGQVLVKLGKCPVCGSPL
SKFKAIQKTEYRIYQCRICNYIHDEKTEKIPLKDLKECPDCGSDISNFEPAETNENNWLISFPKQYIRNDESVRHMDTIY
KLCDSGKPITAPMATELPMPDWDDILILGNQLNPMPLEEDAEVSATTVIGKNAEKPLVIENPVYVSHMSYGALSKESKIA
LAKGSFKAKTAMCSGEGGILPEVKNEAYKYIFEYVPNKYSVTDENLKTSDAIEIKIGQATKPGMGGLLPGDKVTPEIAKV
RGKKAGEDIISPSRFPNINSKKDLKDLVDELRLKSEGRPIGIKIAAGYIENDLEFISYAKPDFITVDGRGGATGASPLLV
RDSTSIPTIFALHRARKYLDEHDLDIDLVITGGLRVSSDFAKALAMGADAIAIASASLIAVACQQYRLCDKGQCPVGVAT
QDKELRSRLKTDIGAKRLTNYLNASLEEIKTFARITGHSDIHDLNVSNLATFNSEISDYTNIKHV

Sequences:

>Translated_545_residues
MKYQCQICGYIHDEEKEGKSFKELEKCPDCKSDVSNFEIMKKDMAYKTYECEVCHYVYDEEKEGQVLVKLGKCPVCGSPL
SKFKAIQKTEYRIYQCRICNYIHDEKTEKIPLKDLKECPDCGSDISNFEPAETNENNWLISFPKQYIRNDESVRHMDTIY
KLCDSGKPITAPMATELPMPDWDDILILGNQLNPMPLEEDAEVSATTVIGKNAEKPLVIENPVYVSHMSYGALSKESKIA
LAKGSFKAKTAMCSGEGGILPEVKNEAYKYIFEYVPNKYSVTDENLKTSDAIEIKIGQATKPGMGGLLPGDKVTPEIAKV
RGKKAGEDIISPSRFPNINSKKDLKDLVDELRLKSEGRPIGIKIAAGYIENDLEFISYAKPDFITVDGRGGATGASPLLV
RDSTSIPTIFALHRARKYLDEHDLDIDLVITGGLRVSSDFAKALAMGADAIAIASASLIAVACQQYRLCDKGQCPVGVAT
QDKELRSRLKTDIGAKRLTNYLNASLEEIKTFARITGHSDIHDLNVSNLATFNSEISDYTNIKHV
>Mature_545_residues
MKYQCQICGYIHDEEKEGKSFKELEKCPDCKSDVSNFEIMKKDMAYKTYECEVCHYVYDEEKEGQVLVKLGKCPVCGSPL
SKFKAIQKTEYRIYQCRICNYIHDEKTEKIPLKDLKECPDCGSDISNFEPAETNENNWLISFPKQYIRNDESVRHMDTIY
KLCDSGKPITAPMATELPMPDWDDILILGNQLNPMPLEEDAEVSATTVIGKNAEKPLVIENPVYVSHMSYGALSKESKIA
LAKGSFKAKTAMCSGEGGILPEVKNEAYKYIFEYVPNKYSVTDENLKTSDAIEIKIGQATKPGMGGLLPGDKVTPEIAKV
RGKKAGEDIISPSRFPNINSKKDLKDLVDELRLKSEGRPIGIKIAAGYIENDLEFISYAKPDFITVDGRGGATGASPLLV
RDSTSIPTIFALHRARKYLDEHDLDIDLVITGGLRVSSDFAKALAMGADAIAIASASLIAVACQQYRLCDKGQCPVGVAT
QDKELRSRLKTDIGAKRLTNYLNASLEEIKTFARITGHSDIHDLNVSNLATFNSEISDYTNIKHV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=304, Percent_Identity=33.8815789473684, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17570289, Length=314, Percent_Identity=31.2101910828025, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320030, Length=316, Percent_Identity=32.5949367088608, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28574881, Length=306, Percent_Identity=28.7581699346405, Blast_Score=122, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24665539, Length=306, Percent_Identity=28.7581699346405, Blast_Score=122, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 60527; Mature: 60527

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50903 RUBREDOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQCQICGYIHDEEKEGKSFKELEKCPDCKSDVSNFEIMKKDMAYKTYECEVCHYVYDE
CCEEEEEECEEECCHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEECCC
EKEGQVLVKLGKCPVCGSPLSKFKAIQKTEYRIYQCRICNYIHDEKTEKIPLKDLKECPD
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEEHHHHHHCCCCCCCCHHHHHHCCC
CGSDISNFEPAETNENNWLISFPKQYIRNDESVRHMDTIYKLCDSGKPITAPMATELPMP
CCCCCCCCCCCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCC
DWDDILILGNQLNPMPLEEDAEVSATTVIGKNAEKPLVIENPVYVSHMSYGALSKESKIA
CCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCEEEEECCCCCCCCCCEEE
LAKGSFKAKTAMCSGEGGILPEVKNEAYKYIFEYVPNKYSVTDENLKTSDAIEIKIGQAT
EECCCCCHHEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCC
KPGMGGLLPGDKVTPEIAKVRGKKAGEDIISPSRFPNINSKKDLKDLVDELRLKSEGRPI
CCCCCCCCCCCCCCHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
GIKIAAGYIENDLEFISYAKPDFITVDGRGGATGASPLLVRDSTSIPTIFALHRARKYLD
EEEEEECCHHCCHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHH
EHDLDIDLVITGGLRVSSDFAKALAMGADAIAIASASLIAVACQQYRLCDKGQCPVGVAT
HCCCCEEEEEECCEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QDKELRSRLKTDIGAKRLTNYLNASLEEIKTFARITGHSDIHDLNVSNLATFNSEISDYT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCHHHHHCC
NIKHV
CCCCC
>Mature Secondary Structure
MKYQCQICGYIHDEEKEGKSFKELEKCPDCKSDVSNFEIMKKDMAYKTYECEVCHYVYDE
CCEEEEEECEEECCHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEECCC
EKEGQVLVKLGKCPVCGSPLSKFKAIQKTEYRIYQCRICNYIHDEKTEKIPLKDLKECPD
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEEHHHHHHCCCCCCCCHHHHHHCCC
CGSDISNFEPAETNENNWLISFPKQYIRNDESVRHMDTIYKLCDSGKPITAPMATELPMP
CCCCCCCCCCCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCC
DWDDILILGNQLNPMPLEEDAEVSATTVIGKNAEKPLVIENPVYVSHMSYGALSKESKIA
CCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCEEEEECCCCCCCCCCEEE
LAKGSFKAKTAMCSGEGGILPEVKNEAYKYIFEYVPNKYSVTDENLKTSDAIEIKIGQAT
EECCCCCHHEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCC
KPGMGGLLPGDKVTPEIAKVRGKKAGEDIISPSRFPNINSKKDLKDLVDELRLKSEGRPI
CCCCCCCCCCCCCCHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
GIKIAAGYIENDLEFISYAKPDFITVDGRGGATGASPLLVRDSTSIPTIFALHRARKYLD
EEEEEECCHHCCHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHH
EHDLDIDLVITGGLRVSSDFAKALAMGADAIAIASASLIAVACQQYRLCDKGQCPVGVAT
HCCCCEEEEEECCEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QDKELRSRLKTDIGAKRLTNYLNASLEEIKTFARITGHSDIHDLNVSNLATFNSEISDYT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCHHHHHCC
NIKHV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]