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The map label for this gene is lysS

Identifier: 148381459

GI number: 148381459

Start: 3760733

End: 3762247

Strand: Reverse

Name: lysS

Synonym: CBO3518

Alternate gene names: 148381459

Gene position: 3762247-3760733 (Counterclockwise)

Preceding gene: 148381460

Following gene: 148381458

Centisome position: 96.79

GC content: 29.9

Gene sequence:

>1515_bases
GTGTCAAAGGAAGATAACGTGATGAACAGTTTTGAAGAACAAGCAAATGAATTAATGAAGGAAAGATTTCAAAAGTTAAA
GGAACTTCAATCTAATGGCAAAGATCCGTTTGATGTTTATAAGGTTGAAAGAACCCATACTTCAAAAGAAGTAAAGGAAA
ACTATGAAGATCTAGAAGGAAAAACTGTTACAGTAGCAGGAAGACTTATGTCAAAAAGAGTTCATGGAAAAGCTGGATTT
TCAGATATACATGATAGATATGGAAAAATTCAATTATACATAAAGATAAATGATGTAGGAGAAGAAAAACTTAAAGAATA
TAAGACTTTTGATATAGGTGATATAATAAGTGTAACAGGAACAGTTTTTAAAACAAAAACAGGAGAAACATCAATACACA
TAACAGATTTTCAGTTAGTTTGTAAATCTTTAAGACCATTACCAGAAAAGTGGCATGGATTAAAAGATCCAGATTTAAGA
TATAGACAAAGATATGTAGATTTAATAATAAACCAAGATGTTAGAGATACTTTTATGAAGAGAACAGCCATAATTAAAAC
CATGAGGGAGTATTTGGATAATAAGGGATTTTTAGAAGTAGAAACACCAATATTATCACCAATAGCAGGGGGAGCAGCAG
CTAAACCATTTATTACTCATCATAATGCTTTAAATATAGATATGTATCTTAGAATAGCTACTGAATTATATTTAAAAAGA
TTAATAGTAGGTGGATTTGAAAAAGTATATGAAATTGGTAGAAACTTTAGAAACGAAGGAATGGATATAAGACATAATCC
AGAATTTACAGTTATAGAATTATATGAAGCTTATGCAGATTACAATGATATGATGGAAATAACAGAAAATATGATAGCAT
ATATATGTGAAAAAGTTCTAGGTACAACTAAAGTTCAATATGAAGGAACAGAAATAGATTTTACACCACCATGGAGAAGA
TTAACTATGGTAGATGCTGTAAGGGAATATGCAGGAGTAGATTTTAACACTATAAAAGATGACATCGAGGCAAGAACAAT
CGCAAAAGAAAAACATATAGAGTTTAAAAAAGAATTAAAGGATTGCACAAAAGGTGATGTACTTATAGGATTATTTGAGG
AATTCTGTGAAGATAAGTTAATGCAACCTACTTTTATTTGTGATTATCCAGTAGAGAATTCACCTCTTACAAAGAAGAAA
AGAGGAAATGAAGCATTTACTGAAAGATTTGAAGGTTTTGTATTTGGAAGAGAGGTATGTAACGCATATTCTGAATTAAA
TGATTCTATAGTTCAAAAAGAAAGATTCATGCAACAATTAAAGGAAAGAGAATTAGGTGATGATGAAGCCTATATGATGG
ATGACGACTTTATAACTTCATTAGAAGTAGGTATGCCACCAACAGGAGGATTAGGAATAGGTATTGATAGATTAATAATG
TTCTTAACAGATACACATTCTATAAGAGATGTTATACTATTCCCAACTATGAAACCACAACCTAATAATCAATAA

Upstream 100 bases:

>100_bases
TAAAAAAGCTGGAGATATAATAGAAGTAGCAGTTCCAGATGGTGTTAGTAAATATGAAATATTAGAGGTTAATAGAGCAT
AGTATTGGAGGGCGTTTATA

Downstream 100 bases:

>100_bases
ATTTAAAATCTATAAATATATTAGTTACATTTGAAGATGAATAGTTTATATATGAATAGCAAATCATTATAAATAACAAC
AAAAATAAATTAAAAGTAAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSKEDNVMNSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKENYEDLEGKTVTVAGRLMSKRVHGKAGF
SDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTGTVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLR
YRQRYVDLIINQDVRDTFMKRTAIIKTMREYLDNKGFLEVETPILSPIAGGAAAKPFITHHNALNIDMYLRIATELYLKR
LIVGGFEKVYEIGRNFRNEGMDIRHNPEFTVIELYEAYADYNDMMEITENMIAYICEKVLGTTKVQYEGTEIDFTPPWRR
LTMVDAVREYAGVDFNTIKDDIEARTIAKEKHIEFKKELKDCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKK
RGNEAFTERFEGFVFGREVCNAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM
FLTDTHSIRDVILFPTMKPQPNNQ

Sequences:

>Translated_504_residues
MSKEDNVMNSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKENYEDLEGKTVTVAGRLMSKRVHGKAGF
SDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTGTVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLR
YRQRYVDLIINQDVRDTFMKRTAIIKTMREYLDNKGFLEVETPILSPIAGGAAAKPFITHHNALNIDMYLRIATELYLKR
LIVGGFEKVYEIGRNFRNEGMDIRHNPEFTVIELYEAYADYNDMMEITENMIAYICEKVLGTTKVQYEGTEIDFTPPWRR
LTMVDAVREYAGVDFNTIKDDIEARTIAKEKHIEFKKELKDCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKK
RGNEAFTERFEGFVFGREVCNAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM
FLTDTHSIRDVILFPTMKPQPNNQ
>Mature_503_residues
SKEDNVMNSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKENYEDLEGKTVTVAGRLMSKRVHGKAGFS
DIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTGTVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLRY
RQRYVDLIINQDVRDTFMKRTAIIKTMREYLDNKGFLEVETPILSPIAGGAAAKPFITHHNALNIDMYLRIATELYLKRL
IVGGFEKVYEIGRNFRNEGMDIRHNPEFTVIELYEAYADYNDMMEITENMIAYICEKVLGTTKVQYEGTEIDFTPPWRRL
TMVDAVREYAGVDFNTIKDDIEARTIAKEKHIEFKKELKDCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKKR
GNEAFTERFEGFVFGREVCNAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIMF
LTDTHSIRDVILFPTMKPQPNNQ

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI194272210, Length=517, Percent_Identity=43.5203094777563, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI5031815, Length=517, Percent_Identity=43.5203094777563, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI45439306, Length=468, Percent_Identity=23.7179487179487, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI40789249, Length=265, Percent_Identity=27.5471698113208, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1790571, Length=495, Percent_Identity=48.8888888888889, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1789256, Length=495, Percent_Identity=49.4949494949495, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=27.5229357798165, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788173, Length=253, Percent_Identity=24.901185770751, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=507, Percent_Identity=42.4063116370809, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535927, Length=507, Percent_Identity=42.4063116370809, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71994340, Length=502, Percent_Identity=42.6294820717131, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17551876, Length=476, Percent_Identity=23.9495798319328, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=302, Percent_Identity=25.1655629139073, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=466, Percent_Identity=42.9184549356223, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324256, Length=490, Percent_Identity=31.8367346938775, Blast_Score=199, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6323011, Length=477, Percent_Identity=25.7861635220126, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325153, Length=153, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640849, Length=527, Percent_Identity=43.6432637571158, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24640851, Length=527, Percent_Identity=43.6432637571158, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24584738, Length=354, Percent_Identity=25.7062146892655, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_CLOB1 (A7FZA5)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385834.1
- ProteinModelPortal:   A7FZA5
- SMR:   A7FZA5
- STRING:   A7FZA5
- GeneID:   5398018
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3593
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   GHPQMMS
- ProtClustDB:   PRK00484
- BioCyc:   CBOT441770:CLB_3593-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 58445; Mature: 58313

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEDNVMNSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKENYEDLEG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHHHHHHCCC
KTVTVAGRLMSKRVHGKAGFSDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTG
CEEEEHHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHHHCC
TVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLRYRQRYVDLIINQDVRDTFMK
CEEEECCCCCEEEEEHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
RTAIIKTMREYLDNKGFLEVETPILSPIAGGAAAKPFITHHNALNIDMYLRIATELYLKR
HHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCEEECCCEEEHHHHHHHHHHHHHHH
LIVGGFEKVYEIGRNFRNEGMDIRHNPEFTVIELYEAYADYNDMMEITENMIAYICEKVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHH
GTTKVQYEGTEIDFTPPWRRLTMVDAVREYAGVDFNTIKDDIEARTIAKEKHIEFKKELK
CCCEEEECCCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKKRGNEAFTERFEGFVFGREVC
HCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
NAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHEECCCCCCCCCCCCHHHHHH
FLTDTHSIRDVILFPTMKPQPNNQ
HHHCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
SKEDNVMNSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKENYEDLEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHHHHHHCCC
KTVTVAGRLMSKRVHGKAGFSDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTG
CEEEEHHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHHHCC
TVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLRYRQRYVDLIINQDVRDTFMK
CEEEECCCCCEEEEEHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
RTAIIKTMREYLDNKGFLEVETPILSPIAGGAAAKPFITHHNALNIDMYLRIATELYLKR
HHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCCCCCEEECCCEEEHHHHHHHHHHHHHHH
LIVGGFEKVYEIGRNFRNEGMDIRHNPEFTVIELYEAYADYNDMMEITENMIAYICEKVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHH
GTTKVQYEGTEIDFTPPWRRLTMVDAVREYAGVDFNTIKDDIEARTIAKEKHIEFKKELK
CCCEEEECCCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKKRGNEAFTERFEGFVFGREVC
HCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
NAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHEECCCCCCCCCCCCHHHHHH
FLTDTHSIRDVILFPTMKPQPNNQ
HHHCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA