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The map label for this gene is greA

Identifier: 148381460

GI number: 148381460

Start: 3762266

End: 3762748

Strand: Reverse

Name: greA

Synonym: CBO3519

Alternate gene names: 148381460

Gene position: 3762748-3762266 (Counterclockwise)

Preceding gene: 148381461

Following gene: 148381459

Centisome position: 96.81

GC content: 28.99

Gene sequence:

>483_bases
ATGAGCGAAGCAAAAAAATATGTTATGACTTATGAAGGGGTAAAGAAATTAGAAGAAGAATTAGAATTTCTTAAAACGGT
AAAAAGAAAAGAAATAACAGAAAAAATAAAAGTAGCTCTTTCATTTGGTGATTTAAGCGAAAATTCAGAATATGATGAAG
CTAAAAATGAACAAGCTTTTGTAGAAGGTAGAATAATACAACTAGAAAATATGCTAAAAAATGCTAGTATAGTTGATGAA
AATGAAGTGCCTAAAGATGTAGTGAGTGTAGGTTCTATAGTAAAAGTTAAAGATTATGAATTTGATGAAGAAGTTGAATA
TATAATTGTAGGCTCTGCTGAAGCTGACCCTATGAATAATAAAATATCTAATGAATCACCAGTAGGACATGGATTAATAG
GTAAAAAAGCTGGAGATATAATAGAAGTAGCAGTTCCAGATGGTGTTAGTAAATATGAAATATTAGAGGTTAATAGAGCA
TAG

Upstream 100 bases:

>100_bases
AAATTACCTGTTTTATAATTACTTAAATGTTTCTTTTATGTTATAATGTTAAGAATGTATCATAATATATAAATAGTAAC
AAAATAAAGGGGAGAATAGC

Downstream 100 bases:

>100_bases
TATTGGAGGGCGTTTATAGTGTCAAAGGAAGATAACGTGATGAACAGTTTTGAAGAACAAGCAAATGAATTAATGAAGGA
AAGATTTCAAAAGTTAAAGG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MSEAKKYVMTYEGVKKLEEELEFLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAFVEGRIIQLENMLKNASIVDE
NEVPKDVVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNNKISNESPVGHGLIGKKAGDIIEVAVPDGVSKYEILEVNRA

Sequences:

>Translated_160_residues
MSEAKKYVMTYEGVKKLEEELEFLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAFVEGRIIQLENMLKNASIVDE
NEVPKDVVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNNKISNESPVGHGLIGKKAGDIIEVAVPDGVSKYEILEVNRA
>Mature_159_residues
SEAKKYVMTYEGVKKLEEELEFLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAFVEGRIIQLENMLKNASIVDEN
EVPKDVVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNNKISNESPVGHGLIGKKAGDIIEVAVPDGVSKYEILEVNRA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=150, Percent_Identity=44, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI87082261, Length=149, Percent_Identity=32.8859060402685, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_CLOB1 (A7FZA6)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385835.1
- ProteinModelPortal:   A7FZA6
- SMR:   A7FZA6
- STRING:   A7FZA6
- GeneID:   5396737
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3594
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   CBOT441770:CLB_3594-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17874; Mature: 17743

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKKYVMTYEGVKKLEEELEFLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHCCCHHH
VEGRIIQLENMLKNASIVDENEVPKDVVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNN
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCEEEEECCCCCCCCC
KISNESPVGHGLIGKKAGDIIEVAVPDGVSKYEILEVNRA
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCC
>Mature Secondary Structure 
SEAKKYVMTYEGVKKLEEELEFLKTVKRKEITEKIKVALSFGDLSENSEYDEAKNEQAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHCCCHHH
VEGRIIQLENMLKNASIVDENEVPKDVVSVGSIVKVKDYEFDEEVEYIIVGSAEADPMNN
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCEEEEECCCCCCCCC
KISNESPVGHGLIGKKAGDIIEVAVPDGVSKYEILEVNRA
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA