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The map label for this gene is murD

Identifier: 148381457

GI number: 148381457

Start: 3756820

End: 3758196

Strand: Reverse

Name: murD

Synonym: CBO3516

Alternate gene names: 148381457

Gene position: 3758196-3756820 (Counterclockwise)

Preceding gene: 148381458

Following gene: 148381456

Centisome position: 96.69

GC content: 28.54

Gene sequence:

>1377_bases
ATGAAAAGCAATTTTTCTAAGTTTAAGGATTTTATAAAGTATAAAAAAGTAGCAGTGGTAGGTATAGGGGTAAGCAATAG
ACCTTTAATAAAATTTTTAGTTAAGTTAGGTGCAAAAGTAACTGCTTTTGACAAAAAACATAGAGAAAAATTAGGAAGTA
TAAGTCTTGAATTAGAAGAGATAGGTGTAGATTTAGTATTAGGTGAAAATTATTTAGATAAATTAGATGGATATGATGTT
ATTTTTAAAACTCCATCTATGAGAATAGATAGACCGGAATTTGTTAAGGCAAAAGAATCTGGAGCTTATATTACATCAGA
AATGGAAGAGTTTATAAAATATTGTCCAGCTAAAGTTTTTGGTATAACAGGAAGTGATGGTAAAACAACTACTACAACAT
TAGTGTATGAAATGTTAAAAAAAGAAGGCTATAGAACTTGGGTAGGGGGAAATATAGGAACACCTTTATTTGCTAATATT
GAAGAAATGAAGGAAGACCATATGGTTGTATTAGAACTATCAAGTTTTCAGCTTATGACAATGGATGTATCACCAGAGAT
TTCTTTAATCACTAATTTAAGTCCTAATCATTTAGATGTGCATAAAGATTTTGAGGAATATGTATGGGCTAAAAAAAATA
TATTTAAGTATCAATCAAGTAATAACTTGTTAGTATTAAATAAAGATGATGATTTAACAAATGGGATGGAGAATGAAGCT
TTAGGAGATGTTTTAAAGTTTAGTCTTGTAGAAAAAGTATATAATGGGGCTTGCTTATCAAATAATAAGCTTACTATACA
AGGAAAAGAAGTATGTGATTCTAAAGATATAAAGCTTAAGGGTAGGCATAATATAGCTAATTTATTAGCAGCTTTTTGTA
TGGTAAATAAATATGTATCAATAGATAGTATGAAATATGTAGCTACAAATTTTTCAGGTGTAGAACATAGATGCGAATTT
ATAAGAGAAGTTAATGGGATTAAATACTATAATGATTCAATAGCATCAAGCCCTAGTAGAACTTTAGCAGGATTAAATTC
TTTTGAAAAGCCAGTAATATTAATAGCTGGTGGATATGATAAAAAGATACCTTTTGAACCCTTGGCAGAAGGTGGATATG
ATAAAATTAAAATATTGATATTAATGGGGGATACGAAAAATAAAATAAAATCAGCTTTTGAAAAAGTAATCTCCTATAAA
AAATGTGAGATGGAAATAGTTATAGTAAATAGCATGGAGGAAGCTGTGAAAGTAGCTGATAATATGGCAGAAAAGGGAGA
TATAATAACTTTATCACCAGCTTGTGCTAGCTTTGATATGTATCCTAACTTTGAAATAAGGGGAAATGAATTTAAGAATA
TAGTAAATAGTTTATAA

Upstream 100 bases:

>100_bases
ATTTATAATAATTACAATATAAAAGTATTTAAAATGTTTTATAAATAAATTAAAGTGATATAATATTAGGTGGATTAATA
CTTAGTTTGGAGGTTTATAA

Downstream 100 bases:

>100_bases
TTTTGTATATTAGATTTATAGAGTTATATCTAAAATTTTTAGAATATAATGTATGTGTAATTCCCTAATTAAAAGTAGTT
TAATATTAAAAGTTCTGCTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISLELEEIGVDLVLGENYLDKLDGYDV
IFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVFGITGSDGKTTTTTLVYEMLKKEGYRTWVGGNIGTPLFANI
EEMKEDHMVVLELSSFQLMTMDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDIKLKGRHNIANLLAAFCMVNKYVSIDSMKYVATNFSGVEHRCEF
IREVNGIKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYDKKIPFEPLAEGGYDKIKILILMGDTKNKIKSAFEKVISYK
KCEMEIVIVNSMEEAVKVADNMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL

Sequences:

>Translated_458_residues
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISLELEEIGVDLVLGENYLDKLDGYDV
IFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVFGITGSDGKTTTTTLVYEMLKKEGYRTWVGGNIGTPLFANI
EEMKEDHMVVLELSSFQLMTMDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDIKLKGRHNIANLLAAFCMVNKYVSIDSMKYVATNFSGVEHRCEF
IREVNGIKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYDKKIPFEPLAEGGYDKIKILILMGDTKNKIKSAFEKVISYK
KCEMEIVIVNSMEEAVKVADNMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL
>Mature_458_residues
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISLELEEIGVDLVLGENYLDKLDGYDV
IFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVFGITGSDGKTTTTTLVYEMLKKEGYRTWVGGNIGTPLFANI
EEMKEDHMVVLELSSFQLMTMDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDIKLKGRHNIANLLAAFCMVNKYVSIDSMKYVATNFSGVEHRCEF
IREVNGIKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYDKKIPFEPLAEGGYDKIKILILMGDTKNKIKSAFEKVISYK
KCEMEIVIVNSMEEAVKVADNMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=30.4157549234136, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_CLOB1 (A7FZA3)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385832.1
- ProteinModelPortal:   A7FZA3
- SMR:   A7FZA3
- STRING:   A7FZA3
- GeneID:   5395511
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3591
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK01710
- BioCyc:   CBOT441770:CLB_3591-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 51464; Mature: 51464

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISLELEE
CCCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHCCEEEEHHH
IGVDLVLGENYLDKLDGYDVIFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVF
CCEEEEECCHHHHHCCCCEEEEECCCCCCCCHHHHEECCCCCCHHHHHHHHHHHCCCEEE
GITGSDGKTTTTTLVYEMLKKEGYRTWVGGNIGTPLFANIEEMKEDHMVVLELSSFQLMT
EEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCEEEEEECCEEEEE
MDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
EECCCCEEEEECCCCCCEEHHCCHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCCHHH
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDIKLKGRHNIANLLAAFCMVNKYVS
HHHHHHHHHHHHHHCCCEECCCEEEEEHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCC
IDSMKYVATNFSGVEHRCEFIREVNGIKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYD
CCCEEEECCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHCCCCCCCEEEEECCCC
KKIPFEPLAEGGYDKIKILILMGDTKNKIKSAFEKVISYKKCEMEIVIVNSMEEAVKVAD
CCCCCCHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHH
NMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL
HHHHCCCEEEECCCCCCCCCCCCEEECCHHHHHHHHCC
>Mature Secondary Structure
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISLELEE
CCCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHCCEEEEHHH
IGVDLVLGENYLDKLDGYDVIFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVF
CCEEEEECCHHHHHCCCCEEEEECCCCCCCCHHHHEECCCCCCHHHHHHHHHHHCCCEEE
GITGSDGKTTTTTLVYEMLKKEGYRTWVGGNIGTPLFANIEEMKEDHMVVLELSSFQLMT
EEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCEEEEEECCEEEEE
MDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
EECCCCEEEEECCCCCCEEHHCCHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCCHHH
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDIKLKGRHNIANLLAAFCMVNKYVS
HHHHHHHHHHHHHHCCCEECCCEEEEEHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCC
IDSMKYVATNFSGVEHRCEFIREVNGIKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYD
CCCEEEECCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHCCCCCCCEEEEECCCC
KKIPFEPLAEGGYDKIKILILMGDTKNKIKSAFEKVISYKKCEMEIVIVNSMEEAVKVAD
CCCCCCHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHH
NMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL
HHHHCCCEEEECCCCCCCCCCCCEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA