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The map label for this gene is fusB [H]

Identifier: 148381456

GI number: 148381456

Start: 3743733

End: 3745805

Strand: Reverse

Name: fusB [H]

Synonym: CBO3515

Alternate gene names: 148381456

Gene position: 3745805-3743733 (Counterclockwise)

Preceding gene: 148381457

Following gene: 148381455

Centisome position: 96.37

GC content: 30.2

Gene sequence:

>2073_bases
ATGAATTATAGCACCACCAATCTAAGGAATGTAGGCATAATTGGACATAGTGGATCAGGAAAAACTTCTTTAACGGAAGC
TATTTTATATTTTACAAGCATGGTAGATAGATTAGGTACCATAGAAGAGGGAAATACTGTATCTGATTATGATACAGAGG
AAAAAAAACGTAAAATATCTATATCTACCACAATACAACCGTGTAAATGGAAAGACACAAAAATTAATTTGATTGATATG
CCTGGTTATTTTGATTTTGTAGGAGAAAAGATTCAAGGGTTAAAAGCTGCTGATATTGCTCTTATAGTATTGTCAGCAAC
AGGAAATATACATGTTGGAACAGAAAAATCTTGGAATTATACAACAAAAGAGAAGATACCTAAAGCATTTTATATAAACA
AATTAGATAAAGAAAATATGGATTTTGAAGGCACTTTGAATAAACTTAAAGAAAGATATGGTACATCTGTAGTACCTGTT
CAATACCCTATAGGAAAAGAAGAGAATTTTAAAGGTGTAATTAATGTTATTTCTAATAAAGCAACTATATTTAATGATAA
AACACATAAGATAGAAATAAAAGAAGTACCAAATGAATTATTAGATGAAGTTAATGAATGTAAAAATATGATTGTAGAAG
CAGTAGCTGAAACAGATGAGAAGCTTTTAGAAAAATATTTTGATGAAGGTACACTAAGTGAAAAGGATATATATGAAGGA
TTGATGGCAGGAACTGTAAAAGGAGAAATAGTTCCGGTAATGTGTGGGTCAGCTATAAAGGCTATAGGTATTGAAACTTT
TTTAGAGGATATAGTTCGATGGTTTCCATCACCAGCAGAAACAAAAAAAATTGAAGTATCAGATGGTATAAATAAAAATA
ATATAGAAATAAATTGTAATGAAAAAGAACCATTTTCAGCACTGGTTTTTAAAACTATTATAGATCCTTTTATAGGTAGA
TTATCATTATTCAAAGTAACGAGTGGAGTGGTAAATGAAGAGAGCTCTATTTATAATTCAACAAAAGATAAAAAGGAGAA
GGTAGGTAAATTATATTTCCTAAGGGGCAAGCAACAAATTCAAACAGATAGAGTAATTTCAGGAGATATAGGGGCAGTTA
GTAAATTACAATATACCTTTACAGGAGATACTTTGTGTGACTTAAAAAGAAAAATATTATTGGAAAATATAGATTTTCCT
AAAGCTAATATGACTATGGCAGCATTACCTAAATCAAAAAATGATGAAGATAAAATGTTTTATGCCCTAGACAAATTGGT
GGAGGAGGACCCTACGATATATATAAGTAGAGATTTAGAAAATGCAGAAACATTAATATCAGGTATGGGAGATATACATT
TAGAAGTAATAGCAAATAGAGTGAAAAATAAATTTGGGACAGAAATAGTATTGGATTCACCTAAAATTCCATATAGGGAG
ACTATAAAATCTATATCTAAGGTTCAAGGAAAGCATAAAAAGCAATCTGGCGGACATGGTCAATACGGAGATGTATTTAT
AAAATTCGAACCAAATAAAAATAGCGAGTTTGAATTTGTGAATAATATAGTTGGAGGGGTAGTTCCTAAACAATATATTC
CTGCAGTAGAAGAAGGAATTTTAGAATGCATGAAGAAGGGAGTCTTAGCAGAATATCCTGTTATAGGAATTAAGGCTACA
ATATACGATGGTTCATATCATTCAGTAGATTCATCTGAAATGGCTTTTAAAATTGCAGCATCTATTGCATTTAAAAAAGG
AATAAAGGAAGCAAAGCCAACTTTATTAGAACCTATAATGCGTTTAGAAGTGATATCATCAGAGGAATATATGGGGGATA
TTATAGGAGATATAAATAGAAAAAGAGGTAAAATTCTAGGCATGGAGCAGGAAGCTAATAATTTAGAAAGAATTGTAGCA
GAAGTACCTCAGGCGGAAATCCTAAATTATTCTACAGATTTAAGGAGTATAACTCAAGGTATGGGAGAATTTTCTTTAAG
CTTTGAAAGATATGATGAAATTTTAGATCCTATTGAGGTTGAGAAAGTATTATCTAGTAAAAATAAAATATAG

Upstream 100 bases:

>100_bases
GTATAGTCTGATTATTAAGAAAATTAGTAATTTTTGAAAGGATTTTATATTATCAAATAGAATATATATTTAAGCCATAA
AATAGATAGGGGGAATTAGT

Downstream 100 bases:

>100_bases
TATAAAAATCACCTAGAAACTAGGTGATTTTTATACTATATTTTATTTAGTGTTTTTTATTATTTTAGTATTTCCATTTA
GATGAAATACCTTTTGAAAT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIGKEENFKGVINVISNKATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKIEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVVNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEIVLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLAEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI

Sequences:

>Translated_690_residues
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIGKEENFKGVINVISNKATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKIEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVVNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEIVLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLAEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI
>Mature_690_residues
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIGKEENFKGVINVISNKATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKIEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVVNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEIVLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLAEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=682, Percent_Identity=32.9912023460411, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI19923640, Length=732, Percent_Identity=27.3224043715847, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI25306283, Length=397, Percent_Identity=29.2191435768262, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI25306287, Length=292, Percent_Identity=31.1643835616438, Blast_Score=156, Evalue=8e-38,
Organism=Homo sapiens, GI157426893, Length=294, Percent_Identity=23.1292517006803, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789738, Length=680, Percent_Identity=40.1470588235294, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1790835, Length=494, Percent_Identity=24.6963562753036, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI48994988, Length=134, Percent_Identity=32.8358208955224, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=677, Percent_Identity=29.6898079763663, Blast_Score=348, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17556745, Length=708, Percent_Identity=24.0112994350282, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323098, Length=677, Percent_Identity=31.7577548005908, Blast_Score=349, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6322359, Length=773, Percent_Identity=27.0375161707633, Blast_Score=281, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6324707, Length=833, Percent_Identity=22.2088835534214, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=833, Percent_Identity=22.2088835534214, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24582462, Length=685, Percent_Identity=33.1386861313869, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI221458488, Length=711, Percent_Identity=27.4261603375527, Blast_Score=252, Evalue=5e-67,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77289; Mature: 77289

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKIS
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEE
ISTTIQPCKWKDTKINLIDMPGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNY
EEECCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCEEEEEEECCCCEEECCCCCCCC
TTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPVQYPIGKEENFKGVINVISNK
CHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCC
ATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
EEEECCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKIEVSDGINKNNIEINCN
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEECC
EKEPFSALVFKTIIDPFIGRLSLFKVTSGVVNEESSIYNSTKDKKEKVGKLYFLRGKQQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHCCEEEECCCHHH
QTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFPKANMTMAALPKSKNDEDKMF
HHCCEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHH
YALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEIVLDSPKIPYRE
HHHHHHHCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHH
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGI
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCHHHHHH
LECMKKGVLAEYPVIGIKATIYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIM
HHHHHCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHCCCHHHHHHH
RLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVAEVPQAEILNYSTDLRSITQG
HHHHHCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHC
MGEFSLSFERYDEILDPIEVEKVLSSKNKI
HHHHEECHHHHHHHHCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNYSTTNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKIS
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEE
ISTTIQPCKWKDTKINLIDMPGYFDFVGEKIQGLKAADIALIVLSATGNIHVGTEKSWNY
EEECCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCEEEEEEECCCCEEECCCCCCCC
TTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPVQYPIGKEENFKGVINVISNK
CHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCC
ATIFNDKTHKIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
EEEECCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDIVRWFPSPAETKKIEVSDGINKNNIEINCN
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEECC
EKEPFSALVFKTIIDPFIGRLSLFKVTSGVVNEESSIYNSTKDKKEKVGKLYFLRGKQQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHCCEEEECCCHHH
QTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFPKANMTMAALPKSKNDEDKMF
HHCCEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHH
YALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEIVLDSPKIPYRE
HHHHHHHCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHH
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGI
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCHHHHHH
LECMKKGVLAEYPVIGIKATIYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIM
HHHHHCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHCCCHHHHHHH
RLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVAEVPQAEILNYSTDLRSITQG
HHHHHCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHC
MGEFSLSFERYDEILDPIEVEKVLSSKNKI
HHHHEECHHHHHHHHCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA