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The map label for this gene is gutB [H]

Identifier: 148381354

GI number: 148381354

Start: 3655433

End: 3655798

Strand: Reverse

Name: gutB [H]

Synonym: CBO3414

Alternate gene names: 148381354

Gene position: 3655798-3655433 (Counterclockwise)

Preceding gene: 148381355

Following gene: 148381353

Centisome position: 94.05

GC content: 28.42

Gene sequence:

>366_bases
ATGAAAACAATATATAATACAGAGGTAAATAAATTAGGAATTATGGTAGAAGAATTTTATGGAGAAAAAATGATAATCTT
ATTTAAAGATAATGCTCCAGAGGAATTAGCAGATTATTGTGTACTTCATGGGAGAAATGAATTAAATGATGAAATACAAG
TAGGAGATATCTTTAAAATTGATGGAGAAGAATATAAAGTGACTGCTGTAGGAGATGAGGTTCATAAGAATTTACGTGAT
TTAGGACATATTTGTGTAAGATTTGATGGAAGTACAAATGCGGAATTACCAGGAAGCTTATATTTAGAAGATAAAGAAAT
AAAAGAAATAAAAGAAGGAACTGTAATAGAAGTTGAAAGACCTTAG

Upstream 100 bases:

>100_bases
TTAAATATACAATTGAATTTATATTATTTGCTTTATATGTAATTAAAATAATATAAAAGGTTAAATTAATAAAAGTAGTT
TAAAAATAGGAGGAAATATT

Downstream 100 bases:

>100_bases
TTAGAAAAGGAGGGATACTGTGAATTTTAAAGAAAAAACTGTTATAATAACAGGAGGAGCTCAAAGTTTAGGAGAATATA
TTGCTTATAGCTTTGCTGAA

Product: PTS system, glucitol/sorbitol-specific, IIA component

Products: Protein Histidine; Sugar Phosphate. [C]

Alternate protein names: EIIA-Gut; EIII-Gut; PTS system glucitol/sorbitol-specific EIIA component [H]

Number of amino acids: Translated: 121; Mature: 121

Protein sequence:

>121_residues
MKTIYNTEVNKLGIMVEEFYGEKMIILFKDNAPEELADYCVLHGRNELNDEIQVGDIFKIDGEEYKVTAVGDEVHKNLRD
LGHICVRFDGSTNAELPGSLYLEDKEIKEIKEGTVIEVERP

Sequences:

>Translated_121_residues
MKTIYNTEVNKLGIMVEEFYGEKMIILFKDNAPEELADYCVLHGRNELNDEIQVGDIFKIDGEEYKVTAVGDEVHKNLRD
LGHICVRFDGSTNAELPGSLYLEDKEIKEIKEGTVIEVERP
>Mature_121_residues
MKTIYNTEVNKLGIMVEEFYGEKMIILFKDNAPEELADYCVLHGRNELNDEIQVGDIFKIDGEEYKVTAVGDEVHKNLRD
LGHICVRFDGSTNAELPGSLYLEDKEIKEIKEGTVIEVERP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3731

COG function: function code G; Phosphotransferase system sorbitol-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI1789056, Length=119, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004716
- InterPro:   IPR018454
- ProDom:   PD015842 [H]

Pfam domain/function: PF03829 PTSIIA_gutA [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 13821; Mature: 13821

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS51097 PTS_EIIA_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIYNTEVNKLGIMVEEFYGEKMIILFKDNAPEELADYCVLHGRNELNDEIQVGDIFKI
CCCCCCCCCHHHHEEEEHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECEEEEE
DGEEYKVTAVGDEVHKNLRDLGHICVRFDGSTNAELPGSLYLEDKEIKEIKEGTVIEVER
CCCEEEEEEECHHHHHHHHHHCEEEEEECCCCCCCCCCEEEECCHHHHHHCCCCEEEEEC
P
C
>Mature Secondary Structure
MKTIYNTEVNKLGIMVEEFYGEKMIILFKDNAPEELADYCVLHGRNELNDEIQVGDIFKI
CCCCCCCCCHHHHEEEEHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECEEEEE
DGEEYKVTAVGDEVHKNLRDLGHICVRFDGSTNAELPGSLYLEDKEIKEIKEGTVIEVER
CCCEEEEEEECHHHHHHHHHHCEEEEEECCCCCCCCCCEEEECCHHHHHHCCCCEEEEEC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. [C]

General reaction: Transferases; Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3553176; 1630305; 9205837; 9278503 [H]