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The map label for this gene is pemK [H]

Identifier: 148381336

GI number: 148381336

Start: 3637001

End: 3637354

Strand: Reverse

Name: pemK [H]

Synonym: CBO3395A

Alternate gene names: 148381336

Gene position: 3637354-3637001 (Counterclockwise)

Preceding gene: 148381337

Following gene: 148381335

Centisome position: 93.58

GC content: 29.66

Gene sequence:

>354_bases
ATGACACAACAGATAGTAAAAAGAGGAGATATATTTTATGCTGATCTAAGTCCTGTAGTAGGTTCAGAGCAAGGTGGAAT
CAGACCGGTTATAGTTATACAAAATAATATAGGGAATAAATATAGTCCCACAGTAATAATTGCAGCAATAACTTCACAAA
TAAATAAAGCTAAACTACCAACCCATGTAGAAATATCCTCAGAGGATTATGGATTAAATAAAGATTCAGTAGTATTGTTA
GAACAAATAAGGACTTTAGATAAGAGAAGATTAAAAGAAAAAATAGGTCATATGATAGATGAAGATATGAAAAAAGTTGA
TACTGCTATTTTAGTTAGTATGGCTTTGAATTAA

Upstream 100 bases:

>100_bases
ATGGCAAATATAAATAGAGACTATGCAGAACAGGGATTTGAACAAGATGTAAAAGATTTAAAAAACTATGAAGCTATGCT
TTCGGAGAGTGATTTTCCAA

Downstream 100 bases:

>100_bases
ATAGGATAAGGGTTTTTAGCCCTTATCTTTTTTTGATATCATGCTATATCTTAATAAAATAAAAAGGATAATTATAAAAA
TAAAAATATTTTTGATTAAT

Product: PemK family protein

Products: NA

Alternate protein names: Endoribonuclease EndoA; Toxin EndoA [H]

Number of amino acids: Translated: 117; Mature: 116

Protein sequence:

>117_residues
MTQQIVKRGDIFYADLSPVVGSEQGGIRPVIVIQNNIGNKYSPTVIIAAITSQINKAKLPTHVEISSEDYGLNKDSVVLL
EQIRTLDKRRLKEKIGHMIDEDMKKVDTAILVSMALN

Sequences:

>Translated_117_residues
MTQQIVKRGDIFYADLSPVVGSEQGGIRPVIVIQNNIGNKYSPTVIIAAITSQINKAKLPTHVEISSEDYGLNKDSVVLL
EQIRTLDKRRLKEKIGHMIDEDMKKVDTAILVSMALN
>Mature_116_residues
TQQIVKRGDIFYADLSPVVGSEQGGIRPVIVIQNNIGNKYSPTVIIAAITSQINKAKLPTHVEISSEDYGLNKDSVVLLE
QIRTLDKRRLKEKIGHMIDEDMKKVDTAILVSMALN

Specific function: Part of a toxin-antitoxin (TA) module. Has specificity for UAC sequences, yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 12989; Mature: 12858

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQIVKRGDIFYADLSPVVGSEQGGIRPVIVIQNNIGNKYSPTVIIAAITSQINKAKLP
CCHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHCCCC
THVEISSEDYGLNKDSVVLLEQIRTLDKRRLKEKIGHMIDEDMKKVDTAILVSMALN
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQQIVKRGDIFYADLSPVVGSEQGGIRPVIVIQNNIGNKYSPTVIIAAITSQINKAKLP
CHHHHHCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHCCCC
THVEISSEDYGLNKDSVVLLEQIRTLDKRRLKEKIGHMIDEDMKKVDTAILVSMALN
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]