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The map label for this gene is ligA

Identifier: 148381215

GI number: 148381215

Start: 3474491

End: 3476485

Strand: Reverse

Name: ligA

Synonym: CBO3270

Alternate gene names: 148381215

Gene position: 3476485-3474491 (Counterclockwise)

Preceding gene: 148381216

Following gene: 148381214

Centisome position: 89.44

GC content: 29.27

Gene sequence:

>1995_bases
ATGGATAACAAGTTAGAAAAGATGAAGGAACTTGTAGAAGAATTAAATCAGTATGCCTATGAGTATTATGTATTAGATAA
TCCCAGTATATCTGATAAAGAATATGACTTGAAATATGATGAATTAGTAATTTTGGAGAAGAAAACTGAAGTTACATTAC
CTTATTCACCAACCCAAAGGGTTGGTGATAAAATATTAGGTGAATTTAGTAAATATACTCACAAAGGAAGACTCTGGAGT
TTGGATAAAGCCCAAAATATGGAGCAGTTAATAGAGTGGCATAATAGAAATTTAAAAGTTATAGAGCAATATAATTCTAT
GAGTGAAGATAAATTACCAGAGTTGAGATATATAGTTACTAAAAAATTTGATGGATTAACGGTAAATTGTACCTATGATG
AAAATGGAATTTTAATAAAGAGTGCTACAAGAGGAACGGGAATTATAGGTGAAGATATAACAGCTCAAATAAAAACTATA
AAGACAGTTCCATTAAAAATAAAAAATAACCACGTTATAGAAGTGCATGGAGAAGCCATAATGACTAAAACAGCTTTTGA
AGAATATAATAAAGCTGCTCAAGTTCCACTAAAAAATTTAAGAAATGGAGCAGCAGGAGCTCTTAGAAATTTAGATATTA
AAGAAACTGCTAGAAGGAATTTATCCGCATTTTTTTATGATGTAGGTTATAATGAAGGTCCCGAATTTAAAAGTTATAGA
GAAATGATGAATTTTATAAAAAATATGGGATTGCCTCAGGATAAGTATATTAAAGAATGTACTAACATGGAAGAAGTGGA
AAAAGAAATTGAATATATAGAATCAATAAGAGGAGAACTAGATTATGATATAGATGGAGCAGTAATTGTTGTAGATGATA
TTAAAACAAGAGAGATATTAGGGTATACTATAAAATTCCCCAAATGGGCTATAGCTTATAAATTTGAAGCTAAGGAAATT
ACTACAAAGCTTCTAGATGTAGAATGGAATGTGGGAAGAAGCGGAAGAGTTACTCCAACAGCTTTATTAGAACCCGTAGA
ACTAGGCGGAGTTACAGTAAAAAGAGCTACACTAAATAATATGGATGATATTAAGAGAAAAAATGTAAAATTGGGTGCTA
AGGTTTTAGTTAGAAGATCTAACGATGTTATACCGGAGATAATGGGAGTAGTAGAAGAATCTTTAGAGGAAAGCAAGGAA
ATACAGGCACCAGATAGATGTCCTTATTGTAATAGCCATCTAGTACAAAATGGAGTACATTATTATTGTGAGAATACATT
ATCTTGTAAACCACAGATGGTAAAAAGTATAGTTCATTTTGCTAGTAGAGAAGCTATGAATATAGCAGGATTCAGTGAAA
AAACTGCAGAACAACTTTTTGAAAAATTAGATATAAAATCTATAGCAGATTTGTATAAGATAAAAAAAGAAGAACTATTA
ACTTTAGAAAAATTTAAGGATAAAAAGTCTCAAAATCTTATAGATGCTATACAAAATAGCAAAAATTGTGATTTGGCATC
CTTTATATACGCTTTAGGTATACCTAATGTAGGTAAAAAGACTGCAAATGATTTAGTAATGAAATTTAAAACTTTAGAAT
CTATAAAGAATACAACAATAGAACAATTAGTAGAAGTTCCAGATGTGGGAGAAATAGTAGCTAAGAGCATATATGATTTT
TTTGAAGACGAAAAAGTTATAAGTAACATAGAAGAATTATTAAATTTGGGTGTAAAACCTTATTATGAAGAAGAAAGAAT
TGATGAGAATCCTTTTATGGATAAAACTATAGTTGTAACAGGAAGTTTGAATAATTATAGCAGAGGGGAAATAAAAGATA
AATTACAGTCTTTAGGAGCTAAAGTTTCTAGTAGTGTAAGTAAAAATACAGACTATGTCTTAGTAGGGGAAAAACCAGGA
TCTAAATATGAAAAAGCAATTGAATTAGGAGTAAAAGTAATCAACGAAGAAGAATTTTCTAATAAAATTAAATAA

Upstream 100 bases:

>100_bases
TTTCGGATGACATAAAGTTAACTATTGCTTTTGACCGTAGAGGTGTTAAAATATTAATGCTTAGTATGGCGCCAATTGAA
GCGGTTTAGGAGGGATATAT

Downstream 100 bases:

>100_bases
TAACATTATAGAAGCATATATTTACAATGAATGAAAAAAGGTATATTATTTAATTGGTTTAATTTATAATAATAGCTCAA
AGAATTTGGAGAGGATAAAA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNNHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESKE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKVISNIEELLNLGVKPYYEEERIDENPFMDKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK

Sequences:

>Translated_664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNNHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESKE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKVISNIEELLNLGVKPYYEEERIDENPFMDKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK
>Mature_664_residues
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQRVGDKILGEFSKYTHKGRLWS
LDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVTKKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTI
KTVPLKIKNNHVIEVHGEAIMTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREILGYTIKFPKWAIAYKFEAKEI
TTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNNMDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESKE
IQAPDRCPYCNSHLVQNGVHYYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTIEQLVEVPDVGEIVAKSIYDF
FEDEKVISNIEELLNLGVKPYYEEERIDENPFMDKTIVVTGSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPG
SKYEKAIELGVKVINEEEFSNKIK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=679, Percent_Identity=40.500736377025, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI87082305, Length=452, Percent_Identity=22.3451327433628, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CLOB1 (A7FYL7)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385590.1
- ProteinModelPortal:   A7FYL7
- SMR:   A7FYL7
- STRING:   A7FYL7
- GeneID:   5398157
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3327
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   CBOT441770:CLB_3327-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75848; Mature: 75848

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 122-122 BINDING 144-144 BINDING 178-178 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQR
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEECCCCCHHH
VGDKILGEFSKYTHKGRLWSLDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVT
HHHHHHHHHHHHCCCCCEEECHHHCCHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHE
KKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTIKTVPLKIKNNHVIEVHGEAI
ECCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHEEEEEEEEEEECCCEEEEECCCHH
MTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHH
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREIL
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHH
GYTIKFPKWAIAYKFEAKEITTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNN
EEEEECCCEEEEEEEEHHHHHHHHHEEEECCCCCCCCCHHHHCCCHHHCCEEEEEHHHCC
MDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESKEIQAPDRCPYCNSHLVQNGVH
HHHHHHHCHHHCHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCC
YYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
EEECCCCCCCHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTI
HHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQLVEVPDVGEIVAKSIYDFFEDEKVISNIEELLNLGVKPYYEEERIDENPFMDKTIVVT
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCEEEEE
GSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPGSKYEKAIELGVKVINEEEFS
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHH
NKIK
CCCC
>Mature Secondary Structure
MDNKLEKMKELVEELNQYAYEYYVLDNPSISDKEYDLKYDELVILEKKTEVTLPYSPTQR
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEECCCCCHHH
VGDKILGEFSKYTHKGRLWSLDKAQNMEQLIEWHNRNLKVIEQYNSMSEDKLPELRYIVT
HHHHHHHHHHHHCCCCCEEECHHHCCHHHHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHE
KKFDGLTVNCTYDENGILIKSATRGTGIIGEDITAQIKTIKTVPLKIKNNHVIEVHGEAI
ECCCCEEEEEEECCCCEEEEECCCCCCCCCCCHHHHEEEEEEEEEEECCCEEEEECCCHH
MTKTAFEEYNKAAQVPLKNLRNGAAGALRNLDIKETARRNLSAFFYDVGYNEGPEFKSYR
HHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCHHHHH
EMMNFIKNMGLPQDKYIKECTNMEEVEKEIEYIESIRGELDYDIDGAVIVVDDIKTREIL
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHH
GYTIKFPKWAIAYKFEAKEITTKLLDVEWNVGRSGRVTPTALLEPVELGGVTVKRATLNN
EEEEECCCEEEEEEEEHHHHHHHHHEEEECCCCCCCCCHHHHCCCHHHCCEEEEEHHHCC
MDDIKRKNVKLGAKVLVRRSNDVIPEIMGVVEESLEESKEIQAPDRCPYCNSHLVQNGVH
HHHHHHHCHHHCHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCC
YYCENTLSCKPQMVKSIVHFASREAMNIAGFSEKTAEQLFEKLDIKSIADLYKIKKEELL
EEECCCCCCCHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
TLEKFKDKKSQNLIDAIQNSKNCDLASFIYALGIPNVGKKTANDLVMKFKTLESIKNTTI
HHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQLVEVPDVGEIVAKSIYDFFEDEKVISNIEELLNLGVKPYYEEERIDENPFMDKTIVVT
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCEEEEE
GSLNNYSRGEIKDKLQSLGAKVSSSVSKNTDYVLVGEKPGSKYEKAIELGVKVINEEEFS
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCHHHHH
NKIK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA