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The map label for this gene is pcrA [H]

Identifier: 148381216

GI number: 148381216

Start: 3476497

End: 3478713

Strand: Reverse

Name: pcrA [H]

Synonym: CBO3271

Alternate gene names: 148381216

Gene position: 3478713-3476497 (Counterclockwise)

Preceding gene: 148381217

Following gene: 148381215

Centisome position: 89.5

GC content: 27.74

Gene sequence:

>2217_bases
ATGGATTTAAAAAATAAATTAAATAAAGAACAATATGAAGCTGCTATTACTATAGATGGGCCTTTACTAATACTCGCAGG
AGCAGGTTCAGGCAAGACAAGGGTTTTGACTTATAGAATAGCCCACATGATAGAAAATTTAAATATATATCCTTCTAAAA
TTTTGGCCATAACTTTTACAAATAAGGCTGCTGGAGAAATGAAAGAAAGAATAAAGGCTTTAGTAGGGGATGTGGTAGAA
GGAATGTGGGTTTCTACATTTCACTCTAGCTGTGTAAGGATTTTAAGAAGAGAAATAGATAAGTTAGGTTATGATAAAAA
TTTTACTATATATGATACTTATGATCAAAAAACTTTAGTTAAGCAATGTATGGAAGAATTGAATATAAATGATAAAGAAA
TCACAGACAGAGAAATAATAAATACCATAGGCAATCAAAAGGATAATTTAATATCACCTAAGGAATTTAAAAAAATTTCT
AATGGAAATTATAGAAAAGATAGAATTGCAGATGCATATATTTTATACCAAAAAAAATTAAAGACTAATAATGCATTAGA
TTTTGATGATTTGATATATAAAACTGTGGAGTTATTTAAAACAAATAAAGATGTATTAGAATTTTACCAAAGAAAATTTA
AATATATAATGGTGGATGAATATCAAGATACTAATAAATCTCAATATGAATTAGTTAAATTATTAGCTTTAGTTCATAGA
AATATGTGTGTAGTTGGTGATGATGATCAATGTATATATGAATGGAGAGGGGCAGATATAAGCAATATATTAAATTTTGA
GAAGGATTATAAAGAAGCTAAGGTTATAAAATTAGAGCAAAATTATAGGTCAAAGGGTAATATATTAAATGCTGCTAATG
AGGTTATAAAAAATAATTCTCAAAGAAAAAATAAAGTTTTAAGAACTGAAAATGAAAATGGAAACAAGATAAAAGTATTT
AGAGCATATTCTGATATAGATGAAGCAAAATTTGTAGCTTCCGAAATCAAAAAAATAATAAAGGATAGTGAAAGATCTTT
TAATGATTTTGCAGTTTTATATAGAACTAACGCCCAATCCCGTATTTTTGAAGATGTGTTTATGAAAAGAGATATCCCTT
ATAGATTAATAGGTGGATTAAAGTTTTATGATAGAAAAGAAATAAAGGATTTAATGGCTTATTTAAAGCTTATAAACAAT
CCATTAGATGATATAAGTTTAAGGAGAATAATAAATGTTCCTAAAAGGAGTATAGGTGATGCTACAGTAAAAAAAGTTCA
AGAATTTGCAAATGAAATGGATGAATGTATGTATAGCGTGCTTTTAGATGCGGATCAAATATTAACATTGTCTCAAAGAA
GTATAACATCTATCAAAAAGTTCGTAAGTATAATGAATAGCTTTATAAGAAAAAAAGATGAGATAAGCGTATCTGCTCTT
ATAAAAGAAATATTAGAAGAAACAGGATACTTAAAAGAATTAAAAAATTCTAAGAATCCAGATGATGTAAGTAGAGTAGA
AAATCTGAAAGAATTAGTTTCCGCAGCAGCAGATTTTGAAAGAGAATCAGAAGATAAATCTTTAGGAGCGTTTTTAGAAA
AAGTAGCATTGGTTACAGATATAGATAATTATGATGAAAATTCAGATAGTGTTGCTATGATGACAGTACATAGTGCTAAG
GGATTAGAATTTCCTGTAGTATTTATGGTAGGTATGGAAAATGGAATATTTCCAGGAACACAATCATTGTCAGATCCAAA
AGAAATGGAAGAGTCTAGAAGACTGTGTTATGTTGCTATAACAAGAGCAAAAGAACAGTTATATATAACTTCAGCAGAAA
TTAGAAAAGTATTTGGAAGAACCGTAGCCTATGCTTTATCAGATTTTGTAAGTGAGATATCCAAAGATTTAAAAGATAAC
GTAAATATTACTGGAGAAGTAGCAACTGATAAAGTATTGCATAAAAATTTTGGTATAAGATCACAAAAAACAAATAGTAT
TAATTCTTATAAATCACCTAGCTTAAATAATATACCTAAAAATAATGTGGATTCTAATAAGCTTAATGTAGGTTTAAAGG
TTAAGCATAAAGATTTTGGAATAGGAACTATAGTTAGTATGAGTAAGGTTTCGGATGACATAAAGTTAACTATTGCTTTT
GACCGTAGAGGTGTTAAAATATTAATGCTTAGTATGGCGCCAATTGAAGCGGTTTAG

Upstream 100 bases:

>100_bases
TTTACTAGCACATTTTAAATTTATAAGAATGTTTAACTAATATTTAAAATGATATAATATTAAGAAGAGGTAAAACAGAT
AAACTTAGGGAGCTGATGGA

Downstream 100 bases:

>100_bases
GAGGGATATATATGGATAACAAGTTAGAAAAGATGAAGGAACTTGTAGAAGAATTAAATCAGTATGCCTATGAGTATTAT
GTATTAGATAATCCCAGTAT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFTNKAAGEMKERIKALVGDVVE
GMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLVKQCMEELNINDKEITDREIINTIGNQKDNLISPKEFKKIS
NGNYRKDRIADAYILYQKKLKTNNALDFDDLIYKTVELFKTNKDVLEFYQRKFKYIMVDEYQDTNKSQYELVKLLALVHR
NMCVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNSQRKNKVLRTENENGNKIKVF
RAYSDIDEAKFVASEIKKIIKDSERSFNDFAVLYRTNAQSRIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINN
PLDDISLRRIINVPKRSIGDATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTDIDNYDENSDSVAMMTVHSAK
GLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAITRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDN
VNITGEVATDKVLHKNFGIRSQKTNSINSYKSPSLNNIPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
DRRGVKILMLSMAPIEAV

Sequences:

>Translated_738_residues
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFTNKAAGEMKERIKALVGDVVE
GMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLVKQCMEELNINDKEITDREIINTIGNQKDNLISPKEFKKIS
NGNYRKDRIADAYILYQKKLKTNNALDFDDLIYKTVELFKTNKDVLEFYQRKFKYIMVDEYQDTNKSQYELVKLLALVHR
NMCVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNSQRKNKVLRTENENGNKIKVF
RAYSDIDEAKFVASEIKKIIKDSERSFNDFAVLYRTNAQSRIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINN
PLDDISLRRIINVPKRSIGDATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTDIDNYDENSDSVAMMTVHSAK
GLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAITRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDN
VNITGEVATDKVLHKNFGIRSQKTNSINSYKSPSLNNIPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
DRRGVKILMLSMAPIEAV
>Mature_738_residues
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFTNKAAGEMKERIKALVGDVVE
GMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLVKQCMEELNINDKEITDREIINTIGNQKDNLISPKEFKKIS
NGNYRKDRIADAYILYQKKLKTNNALDFDDLIYKTVELFKTNKDVLEFYQRKFKYIMVDEYQDTNKSQYELVKLLALVHR
NMCVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNSQRKNKVLRTENENGNKIKVF
RAYSDIDEAKFVASEIKKIIKDSERSFNDFAVLYRTNAQSRIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINN
PLDDISLRRIINVPKRSIGDATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTDIDNYDENSDSVAMMTVHSAK
GLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAITRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDN
VNITGEVATDKVLHKNFGIRSQKTNSINSYKSPSLNNIPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
DRRGVKILMLSMAPIEAV

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=739, Percent_Identity=37.0771312584574, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI48994965, Length=640, Percent_Identity=40.78125, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1787196, Length=371, Percent_Identity=28.0323450134771, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=748, Percent_Identity=28.3422459893048, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324477, Length=755, Percent_Identity=23.5761589403974, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84478; Mature: 84478

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFT
CCCHHHCCHHHCCEEEEECCCEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEC
NKAAGEMKERIKALVGDVVEGMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
KQCMEELNINDKEITDREIINTIGNQKDNLISPKEFKKISNGNYRKDRIADAYILYQKKL
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KTNNALDFDDLIYKTVELFKTNKDVLEFYQRKFKYIMVDEYQDTNKSQYELVKLLALVHR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHC
NMCVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNS
CEEEECCCCCEEEEECCCCHHHHHHHHHHHCHHEEEEEHHCCCCCCCHHHHHHHHHHCCC
QRKNKVLRTENENGNKIKVFRAYSDIDEAKFVASEIKKIIKDSERSFNDFAVLYRTNAQS
HHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHH
RIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINNPLDDISLRRIINVPKRSIGD
HHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
ATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTD
HHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
IDNYDENSDSVAMMTVHSAKGLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAI
CCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCEEEEEE
TRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDNVNITGEVATDKVLHKNFGIR
ECCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCC
SQKTNSINSYKSPSLNNIPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEE
DRRGVKILMLSMAPIEAV
CCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFT
CCCHHHCCHHHCCEEEEECCCEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEC
NKAAGEMKERIKALVGDVVEGMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
KQCMEELNINDKEITDREIINTIGNQKDNLISPKEFKKISNGNYRKDRIADAYILYQKKL
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KTNNALDFDDLIYKTVELFKTNKDVLEFYQRKFKYIMVDEYQDTNKSQYELVKLLALVHR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHC
NMCVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNS
CEEEECCCCCEEEEECCCCHHHHHHHHHHHCHHEEEEEHHCCCCCCCHHHHHHHHHHCCC
QRKNKVLRTENENGNKIKVFRAYSDIDEAKFVASEIKKIIKDSERSFNDFAVLYRTNAQS
HHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHH
RIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINNPLDDISLRRIINVPKRSIGD
HHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
ATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTD
HHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
IDNYDENSDSVAMMTVHSAKGLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAI
CCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCEEEEEE
TRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDNVNITGEVATDKVLHKNFGIR
ECCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCC
SQKTNSINSYKSPSLNNIPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEE
DRRGVKILMLSMAPIEAV
CCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]