Click here to switch to the map view.

The map label for this gene is gatA

Identifier: 148381211

GI number: 148381211

Start: 3470697

End: 3472154

Strand: Reverse

Name: gatA

Synonym: CBO3266

Alternate gene names: 148381211

Gene position: 3472154-3470697 (Counterclockwise)

Preceding gene: 148381212

Following gene: 148381210

Centisome position: 89.33

GC content: 31.62

Gene sequence:

>1458_bases
ATGGATTTAACTAAATTAACAGCACACGAATTAAAAGATATGCTTTCAAATAAAGAAGTTAAAGCAGAAGAAATAACAAA
AGCTTTTTTAGATAGAATAAATTTAGTAGACAATAAGCTAGGAGCATACTTATATGTATCAGAGGAAGAAGCTATAAAAA
AAGCTAAGGAAATTGATGGGAAAATAGAAAAAAATGAAGAGTTAAAAGCCTTATCAGGTATACCAGTAGGTATAAAAGAT
AATATAAATGTAAAAGGAATGCAAAATACTTGTGCATCTAAAATATTAGAAGGTTATACTTCACCTTATGATGCTCATGT
AACAGAAAAAATAAAACAAGAAGAAGGAATAATCCTTGGCAAATTAAACATGGATGAGTTTGCTATGGGATCATCTACAG
AAAATAGTGCATTTAAATTAGCCAAAAATCCATGGGATTTAGAAAGAGTACCAGGTGGATCTTCAGGTGGATCTGCAGTG
GCGGTAGCAGGATCTGAAGCTACTTTATCATTAGGAACAGATACTGGTGGTTCTGTAAGACAACCTGCATCCTTTTGTGG
AGTAGTTGGTTTAAAACCTACATATGGAAGAATATCAAGATCAGGGGTTGTAGCTTTTGGTTCTACATTAGATCAAGTAG
GTCCAATGGGAAAAGATGTAGAGGATTGTGCTTTGCTAACATCAGTTATAGCGGGATTAGATAAAAAGGATTTTACAACA
GTAGATAAGGAAGTTCCTGATTATAAAAAAAGTTTAACTAAAGATATAAAGGGTAAAAGAATTGGTATTCCAAAAGAGTT
CTTTGGAGATGGATTAGATAAGAATGTAAGAAAATCTGTAGAAGAAGCTATAAAGGTATTAGAGGCAAATGGAGCAGAAG
TTAAACCATGTTCTCTACCATTAATGGATTATGCCCTATCAGCTTATTACATAATATCTAGTGCAGAAGCTTCATCAAAT
TTAGCTAGATTTGACGGTATAAGATATGGTTATAGATCAAAAAACTTTAAAGATGCTAAAGATATATATTTGAAATCTAG
AAGTGAAGGCTTTGGAGATGAAGTTAAGAGAAGAATAATGCTTGGAACTTATGTATTATCAGCAGGATACTATGATGCTT
ACTACAAGAAAGCATTAAAAGTAAGAAAACTTATAAAAGACGATTTCCAAAGAGTATTTAAAGAATTTGATGCTATAGTG
TCACCAACATCTCCAACTACAGCATTTAAAGTAGGAGAAAAGAAAGACGATGTAATGTCAATGTATCTTTCAGATATATA
TACTGTTCCAATTAGTGTAGCAGGAGTACCAGCTATATCATTACCATGTGGTATGATAGATGGACTTCCAGTAGGGCTTC
AAATTATATCAGATTATTTTAAAGAAGATGTATTATTTAATTTAGCATATAATTATGAACAATCAGTAGATTTTCATAAA
ATGAGAGCGGATTTTTAA

Upstream 100 bases:

>100_bases
AGTTATAGATAATGCACCAGAAAGCTTAGAAGAATATGTAATTGTTCCTAAAGTTATAGATTAATAATTCTTTTGTTTTC
TGGAAAAGGGAGGAAAGAAA

Downstream 100 bases:

>100_bases
GTATATAGTTAAAAACAAAGGAGTGAAAAACATAGATGGATTTTGAAGCAGTTATAGGATTGGAAGTTCATGCTGAACTT
TCAACAAATACTAAAATATA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDGKIEKNEELKALSGIPVGIKD
NINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGSEATLSLGTDTGGSVRQPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNVRKSVEEAIKVLEANGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGYRSKNFKDAKDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKEFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
MRADF

Sequences:

>Translated_485_residues
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDGKIEKNEELKALSGIPVGIKD
NINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGSEATLSLGTDTGGSVRQPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNVRKSVEEAIKVLEANGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGYRSKNFKDAKDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKEFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
MRADF
>Mature_485_residues
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDGKIEKNEELKALSGIPVGIKD
NINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGSEATLSLGTDTGGSVRQPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNVRKSVEEAIKVLEANGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGYRSKNFKDAKDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKEFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
MRADF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=465, Percent_Identity=36.1290322580645, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=23.943661971831, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI166795287, Length=232, Percent_Identity=31.4655172413793, Blast_Score=82, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=435, Percent_Identity=37.7011494252874, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17537465, Length=506, Percent_Identity=24.901185770751, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=293, Percent_Identity=28.6689419795222, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=318, Percent_Identity=29.2452830188679, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=317, Percent_Identity=27.1293375394322, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=304, Percent_Identity=27.9605263157895, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=304, Percent_Identity=28.6184210526316, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=417, Percent_Identity=38.3693045563549, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6319685, Length=456, Percent_Identity=28.2894736842105, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=523, Percent_Identity=24.4741873804971, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648113, Length=501, Percent_Identity=37.3253493013972, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24648435, Length=498, Percent_Identity=25.1004016064257, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24648437, Length=498, Percent_Identity=25.1004016064257, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI45550774, Length=494, Percent_Identity=25.7085020242915, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24652985, Length=491, Percent_Identity=23.8289205702648, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922090, Length=491, Percent_Identity=23.8289205702648, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652981, Length=491, Percent_Identity=23.8289205702648, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652983, Length=491, Percent_Identity=23.8289205702648, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=24.6124031007752, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=25.3578732106339, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648441, Length=431, Percent_Identity=25.0580046403712, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648439, Length=431, Percent_Identity=25.0580046403712, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078093, Length=507, Percent_Identity=24.0631163708087, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CLOB1 (A7FYL3)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385586.1
- ProteinModelPortal:   A7FYL3
- SMR:   A7FYL3
- STRING:   A7FYL3
- GeneID:   5395335
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3323
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   CBOT441770:CLB_3323-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53183; Mature: 53183

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDG
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCC
KIEKNEELKALSGIPVGIKDNINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILG
CCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEE
KLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAVAVAGSEATLSLGTDTGGSVR
ECCCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCC
QPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
CCHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCH
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNVRKSVEEAIKVLEANGAEVKPCSLP
HHHCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
LMDYALSAYYIISSAEASSNLARFDGIRYGYRSKNFKDAKDIYLKSRSEGFGDEVKRRIM
HHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
LGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKEFDAIVSPTSPTTAFKVGEKKDDVMS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
MYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
HHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHH
MRADF
HHCCC
>Mature Secondary Structure
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDG
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCC
KIEKNEELKALSGIPVGIKDNINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILG
CCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEE
KLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAVAVAGSEATLSLGTDTGGSVR
ECCCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCC
QPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
CCHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCH
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNVRKSVEEAIKVLEANGAEVKPCSLP
HHHCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
LMDYALSAYYIISSAEASSNLARFDGIRYGYRSKNFKDAKDIYLKSRSEGFGDEVKRRIM
HHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
LGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKEFDAIVSPTSPTTAFKVGEKKDDVMS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
MYLSDIYTVPISVAGVPAISLPCGMIDGLPVGLQIISDYFKEDVLFNLAYNYEQSVDFHK
HHHHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHH
MRADF
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA