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The map label for this gene is gatB

Identifier: 148381210

GI number: 148381210

Start: 3469231

End: 3470661

Strand: Reverse

Name: gatB

Synonym: CBO3265

Alternate gene names: 148381210

Gene position: 3470661-3469231 (Counterclockwise)

Preceding gene: 148381211

Following gene: 148381209

Centisome position: 89.29

GC content: 29.84

Gene sequence:

>1431_bases
ATGGATTTTGAAGCAGTTATAGGATTGGAAGTTCATGCTGAACTTTCAACAAATACTAAAATATATTGTGGATGCACTAC
AGAATTTGGTGGTCAACCTAATACTCATGTTTGTCCAATATGTTTAGGATTACCAGGTTCTCTACCTCAATTAAATAAAA
GGGTAGTAGAGTATGGAATTAAGGCAGGTTTAGCTTTAAATTGTTCTATAAACAAAGTTTGTAGAATGGATAGAAAAAAT
TATTTTTATCCAGATTGCCCTAAAAATTATCAAATAACTCAAGATGAAGTTCCAATATGTAGAGATGGATATATTGAAAT
AGAGTTAGAAAACGGTGAAAAGAAAAAAATAGGCATAGAAAGAATACATATGGAAGAAGATGCTGGAAAATTACTTCATA
CTAATGCGGGAACATTGGTAGATTACAATAGAGCAGGAGTACCATTAATAGAGATAGTATCAAGACCAGATATAAGAACT
CCAGAAGAAGCTACAAAATACTTAGAGAAATTAAAGAGTATACTATCTTCAATAGAAGTATCAGATTGTAAAATGGAGCA
AGGTTCCTTAAGATGTGATGGTAATATATCTGTAATGCCTAAAGGTAGCGAAAAATTTGGAGTAAGATCAGAAATAAAAA
ATATGAATTCTTTTAAAGCATTGGAAAAAGCTTTATCATATGAATATGATAGACATGTAGAAGCTGTAACAAAGGGAGAA
ATTTTAGAGCAAGAAACAAGAAGATGGGACGAAGCTAATTCTGTTACAGTGCTTATGCGAAGCAAAGAAAAAGCTAATGA
TTATAGATACTTTCCAGAAGGAGATTTAGTTACATTAAATATTTCAGATGAATGGATAGAAGAAGTAAGAAAAACTATAC
CAGAGTTACCACATGAAAAAGCTGAAAGATTTGTAAATGAATTTGGAATACCTAAATATGATGCTATGGTATTAACTTTA
ACAATGGATATGGCTAAATTTTTTGAAGAAACTGCCCTAAAGAGTGAAGATGCAAAAGCTGCTTCTAACTGGTTAATGGG
TGATATTTCAAGACTTATGAATGAAAAAGCTATAGAAGTTAAAGATTTGAAATTTAATCCTGAACAATTAGCCCAGCTAA
TTAAATTAATAAACGCTGGAACTATATCTAATAATATAGGTAAAAAAGTTTTAGATGATATGTTTAAGTCAGGTAAAAAT
CCTAAAGATATAGTTGAAGAAAAAGGTTTGGTTCAAAATAATGATGAAGGTGCTATATTAGAAGTAGTTAAAAATATTAT
AGAGAATAATCCTCAATCTATAGAAGACTTCAAAAATGGAAAGAAAAGAGCATTAGGTTTCCTTGTAGGTCTTGTAATGA
AAGAAACAAAAGGAAAAGCTAATCCTCAAATAGTAAATAAATTAGTAAGTGAAGAAGCAAATAAAATGTAA

Upstream 100 bases:

>100_bases
TATTTAATTTAGCATATAATTATGAACAATCAGTAGATTTTCATAAAATGAGAGCGGATTTTTAAGTATATAGTTAAAAA
CAAAGGAGTGAAAAACATAG

Downstream 100 bases:

>100_bases
ATATTAGAATAAAAGATCCTCACATAAAAATGTGGGGATTTTTTTATGGAAAATAGTTTGAATTATATGTAAAGTTTAAA
AATGTTGTTTATTTTAACCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGIKAGLALNCSINKVCRMDRKN
YFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIERIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRT
PEEATKYLEKLKSILSSIEVSDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEKAERFVNEFGIPKYDAMVLTL
TMDMAKFFEETALKSEDAKAASNWLMGDISRLMNEKAIEVKDLKFNPEQLAQLIKLINAGTISNNIGKKVLDDMFKSGKN
PKDIVEEKGLVQNNDEGAILEVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM

Sequences:

>Translated_476_residues
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGIKAGLALNCSINKVCRMDRKN
YFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIERIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRT
PEEATKYLEKLKSILSSIEVSDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEKAERFVNEFGIPKYDAMVLTL
TMDMAKFFEETALKSEDAKAASNWLMGDISRLMNEKAIEVKDLKFNPEQLAQLIKLINAGTISNNIGKKVLDDMFKSGKN
PKDIVEEKGLVQNNDEGAILEVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM
>Mature_476_residues
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGIKAGLALNCSINKVCRMDRKN
YFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIERIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRT
PEEATKYLEKLKSILSSIEVSDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEKAERFVNEFGIPKYDAMVLTL
TMDMAKFFEETALKSEDAKAASNWLMGDISRLMNEKAIEVKDLKFNPEQLAQLIKLINAGTISNNIGKKVLDDMFKSGKN
PKDIVEEKGLVQNNDEGAILEVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=490, Percent_Identity=36.3265306122449, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=37.248322147651, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=500, Percent_Identity=29.6, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45550808, Length=487, Percent_Identity=33.8809034907598, Blast_Score=280, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CLOB1 (A7FYL2)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385585.1
- ProteinModelPortal:   A7FYL2
- SMR:   A7FYL2
- STRING:   A7FYL2
- GeneID:   5395604
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3322
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   CBOT441770:CLB_3322-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53657; Mature: 53657

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGI
CCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
KAGLALNCSINKVCRMDRKNYFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIE
HCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
RIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRTPEEATKYLEKLKSILSSIEV
HEEEHHCCCCEEECCCCCEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
CCCCCCCCCEEECCCEEEECCCCHHCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHH
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEK
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHH
AERFVNEFGIPKYDAMVLTLTMDMAKFFEETALKSEDAKAASNWLMGDISRLMNEKAIEV
HHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
KDLKFNPEQLAQLIKLINAGTISNNIGKKVLDDMFKSGKNPKDIVEEKGLVQNNDEGAIL
EECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHH
EVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM
HHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGI
CCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
KAGLALNCSINKVCRMDRKNYFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIE
HCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
RIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRTPEEATKYLEKLKSILSSIEV
HEEEHHCCCCEEECCCCCEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
CCCCCCCCCEEECCCEEEECCCCHHCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHH
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEK
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHH
AERFVNEFGIPKYDAMVLTLTMDMAKFFEETALKSEDAKAASNWLMGDISRLMNEKAIEV
HHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
KDLKFNPEQLAQLIKLINAGTISNNIGKKVLDDMFKSGKNPKDIVEEKGLVQNNDEGAIL
EECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHH
EVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM
HHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA