| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
Click here to switch to the map view.
The map label for this gene is pepD [H]
Identifier: 148380825
GI number: 148380825
Start: 3048275
End: 3049711
Strand: Reverse
Name: pepD [H]
Synonym: CBO2867
Alternate gene names: 148380825
Gene position: 3049711-3048275 (Counterclockwise)
Preceding gene: 148380827
Following gene: 148380824
Centisome position: 78.46
GC content: 29.16
Gene sequence:
>1437_bases ATGTTAGAAAATTTACAACCAAAATCAGTATTTCATTTTTTTGAGGAATTAACTAAGATACCACATGGATCAGGAGATGA AAAAAGAATAAGTGATTACTTAGTTAATTTTGCAAAGGAAAGAAATTTAGAAGTTATTCAAGATAAATCTTTAAATGTAA TAATAAGAAAACCAGCTACAAAAGGATATGAAAGTGCTCCAGCCGTAATTATTCAAGGTCATATGGATATGGTTTGTGAA AAAGTTAAGGAAAGTGATCATGATTTTGAAAAAGATCCTTTAAAATTAAGAATAGATGGGGATTATTTATATGCTACAGG TACAACATTAGGGGGAGATGATGGGATCGCTGTTGCCTATGGACTAGCTGTCTTAGATTCAAAGGATATAGCGCATCCTA GTATAGAATTTGTTGCTACAACTTCAGAAGAAACAGGCATGAATGGTGCTATGGGGCTAGATACTTCTAAATTAAATGGG AAAATTTTGTTGAATATTGATTCAGAAGAGGAAGGAATATTTTTAGTTAGTTGTTCTGGTGGAGTAAATCCTATAGTTTA TATACCAAAAGAGTATGAAAAAGCTACAGGTCAGTCTGTAAAAATAGAAATAAAAGGATTGAATGGTGGACATTCAGGTA TGGAAATTATAAAACAAAGAGCAAATGCTAATAAGTTAATGGGAAGAGTTTTATATGACCTAAAGAAAGAAGTAGATTTC AATCTTGTTGAAATAAATGGAGGATCAAAACATAATGCTATTGCTTTACATGCTGATTCAGTATTAACAATAGATGAAAA TAAATTTAATGAAGTTAAATCTCTTTGTGATAAGCTAGAAGAAACTTTCCAAAATGAATTTAGAGTGGAAGATCCAAATA TAAAAGTTGTAGTTGAAAAGATAGAAAACAGCGAAAAACAGTTAATTAAAAAAGTAACGGACAATATAATAAATTTCTTG GTATTAGTTCCTTATGGAGTTCAAACTATGAGTAAAGATATAGATGGATTAGTTGAAAGTAGTTTAAATATTGGTATAGT TGAAAATAAGGAAGAGGAAATTAAAGTTACAATAGCTGTTAGAAGTTCAGTAAGTAGTTTAAAATTAGAAGTTGTAAATA GAATAGAGGCTTTATGTTCACTAATAGGTGCAAAATGTGTTAGAGAAGATGAGTATCCAGCATGGCAATTTGATCCAGAA TCTAAAATAAAAGATTTATGTATTGATATATATACTAAATTATATGAAAAACCAGATGTATCAGCAATACATGCAGGATT AGAATGTGGATTATTTAAAGGTACTATGAAAGATACTGATATGATAAGTTTTGGACCAAATCTATATGATGTTCATACAC CAAATGAACACTTAAGTATATCTTCAGTAGAAAGAATATGGAAATTCTTAGTGGAACTATTAAAGAACATAAAATAA
Upstream 100 bases:
>100_bases TAGTAGAATTAGGATAATGTCGTGTTAGTATTAAATTATATATAAATTTAAGTATTGGGGTAATTTTAAAATTTAATTTT AAAAAGGGGGATATATTACC
Downstream 100 bases:
>100_bases AAATTAATTATTGACATAGATAAGAAAAAGAGTTATATTAAAATTGTAAAATTTAATAAAATGAATCCTTCAGGGCGGGG CGCAATTCCCCACCGGCGGT
Product: aminoacyl-histidine dipeptidase
Products: NA
Alternate protein names: Beta-alanyl-histidine dipeptidase; Carnosinase; Cysteinylglycinase; Peptidase D; Xaa-His dipeptidase; X-His dipeptidase [H]
Number of amino acids: Translated: 478; Mature: 478
Protein sequence:
>478_residues MLENLQPKSVFHFFEELTKIPHGSGDEKRISDYLVNFAKERNLEVIQDKSLNVIIRKPATKGYESAPAVIIQGHMDMVCE KVKESDHDFEKDPLKLRIDGDYLYATGTTLGGDDGIAVAYGLAVLDSKDIAHPSIEFVATTSEETGMNGAMGLDTSKLNG KILLNIDSEEEGIFLVSCSGGVNPIVYIPKEYEKATGQSVKIEIKGLNGGHSGMEIIKQRANANKLMGRVLYDLKKEVDF NLVEINGGSKHNAIALHADSVLTIDENKFNEVKSLCDKLEETFQNEFRVEDPNIKVVVEKIENSEKQLIKKVTDNIINFL VLVPYGVQTMSKDIDGLVESSLNIGIVENKEEEIKVTIAVRSSVSSLKLEVVNRIEALCSLIGAKCVREDEYPAWQFDPE SKIKDLCIDIYTKLYEKPDVSAIHAGLECGLFKGTMKDTDMISFGPNLYDVHTPNEHLSISSVERIWKFLVELLKNIK
Sequences:
>Translated_478_residues MLENLQPKSVFHFFEELTKIPHGSGDEKRISDYLVNFAKERNLEVIQDKSLNVIIRKPATKGYESAPAVIIQGHMDMVCE KVKESDHDFEKDPLKLRIDGDYLYATGTTLGGDDGIAVAYGLAVLDSKDIAHPSIEFVATTSEETGMNGAMGLDTSKLNG KILLNIDSEEEGIFLVSCSGGVNPIVYIPKEYEKATGQSVKIEIKGLNGGHSGMEIIKQRANANKLMGRVLYDLKKEVDF NLVEINGGSKHNAIALHADSVLTIDENKFNEVKSLCDKLEETFQNEFRVEDPNIKVVVEKIENSEKQLIKKVTDNIINFL VLVPYGVQTMSKDIDGLVESSLNIGIVENKEEEIKVTIAVRSSVSSLKLEVVNRIEALCSLIGAKCVREDEYPAWQFDPE SKIKDLCIDIYTKLYEKPDVSAIHAGLECGLFKGTMKDTDMISFGPNLYDVHTPNEHLSISSVERIWKFLVELLKNIK >Mature_478_residues MLENLQPKSVFHFFEELTKIPHGSGDEKRISDYLVNFAKERNLEVIQDKSLNVIIRKPATKGYESAPAVIIQGHMDMVCE KVKESDHDFEKDPLKLRIDGDYLYATGTTLGGDDGIAVAYGLAVLDSKDIAHPSIEFVATTSEETGMNGAMGLDTSKLNG KILLNIDSEEEGIFLVSCSGGVNPIVYIPKEYEKATGQSVKIEIKGLNGGHSGMEIIKQRANANKLMGRVLYDLKKEVDF NLVEINGGSKHNAIALHADSVLTIDENKFNEVKSLCDKLEETFQNEFRVEDPNIKVVVEKIENSEKQLIKKVTDNIINFL VLVPYGVQTMSKDIDGLVESSLNIGIVENKEEEIKVTIAVRSSVSSLKLEVVNRIEALCSLIGAKCVREDEYPAWQFDPE SKIKDLCIDIYTKLYEKPDVSAIHAGLECGLFKGTMKDTDMISFGPNLYDVHTPNEHLSISSVERIWKFLVELLKNIK
Specific function: Dipeptidase with broad substrate specificity. Requires dipeptide substrates with an unblocked N-terminus and the amino group in the alpha or beta position. Non-protein amino acids and proline are not accepted in the C-terminal position, whereas some dipep
COG id: COG2195
COG function: function code E; Di- and tripeptidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the peptidase M20C family [H]
Homologues:
Organism=Escherichia coli, GI1786432, Length=479, Percent_Identity=39.0396659707724, Blast_Score=360, Evalue=1e-101,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002933 - InterPro: IPR011650 - InterPro: IPR001160 [H]
Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]
EC number: =3.4.13.3 [H]
Molecular weight: Translated: 53232; Mature: 53232
Theoretical pI: Translated: 4.80; Mature: 4.80
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLENLQPKSVFHFFEELTKIPHGSGDEKRISDYLVNFAKERNLEVIQDKSLNVIIRKPAT CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCC KGYESAPAVIIQGHMDMVCEKVKESDHDFEKDPLKLRIDGDYLYATGTTLGGDDGIAVAY CCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHH GLAVLDSKDIAHPSIEFVATTSEETGMNGAMGLDTSKLNGKILLNIDSEEEGIFLVSCSG HHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCC GVNPIVYIPKEYEKATGQSVKIEIKGLNGGHSGMEIIKQRANANKLMGRVLYDLKKEVDF CCCCEEECCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC NLVEINGGSKHNAIALHADSVLTIDENKFNEVKSLCDKLEETFQNEFRVEDPNIKVVVEK EEEEECCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHCCEECCCCCEEEEEH IENSEKQLIKKVTDNIINFLVLVPYGVQTMSKDIDGLVESSLNIGIVENKEEEIKVTIAV HCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEE RSSVSSLKLEVVNRIEALCSLIGAKCVREDEYPAWQFDPESKIKDLCIDIYTKLYEKPDV ECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH SAIHAGLECGLFKGTMKDTDMISFGPNLYDVHTPNEHLSISSVERIWKFLVELLKNIK HHHHCCCHHCEEECCCCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MLENLQPKSVFHFFEELTKIPHGSGDEKRISDYLVNFAKERNLEVIQDKSLNVIIRKPAT CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCC KGYESAPAVIIQGHMDMVCEKVKESDHDFEKDPLKLRIDGDYLYATGTTLGGDDGIAVAY CCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHH GLAVLDSKDIAHPSIEFVATTSEETGMNGAMGLDTSKLNGKILLNIDSEEEGIFLVSCSG HHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCC GVNPIVYIPKEYEKATGQSVKIEIKGLNGGHSGMEIIKQRANANKLMGRVLYDLKKEVDF CCCCEEECCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC NLVEINGGSKHNAIALHADSVLTIDENKFNEVKSLCDKLEETFQNEFRVEDPNIKVVVEK EEEEECCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHCCEECCCCCEEEEEH IENSEKQLIKKVTDNIINFLVLVPYGVQTMSKDIDGLVESSLNIGIVENKEEEIKVTIAV HCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEE RSSVSSLKLEVVNRIEALCSLIGAKCVREDEYPAWQFDPESKIKDLCIDIYTKLYEKPDV ECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH SAIHAGLECGLFKGTMKDTDMISFGPNLYDVHTPNEHLSISSVERIWKFLVELLKNIK HHHHCCCHHCEEECCCCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1695895; 9278503; 2651887 [H]