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The map label for this gene is def [H]

Identifier: 148380578

GI number: 148380578

Start: 2771350

End: 2771820

Strand: Reverse

Name: def [H]

Synonym: CBO2622

Alternate gene names: 148380578

Gene position: 2771820-2771350 (Counterclockwise)

Preceding gene: 148380579

Following gene: 148380577

Centisome position: 71.31

GC content: 31.42

Gene sequence:

>471_bases
ATGGCAATAAGAGAGATATTACAAGTTGGAGACAAGACGTTGAAAAGAGTTAGTAAAAAAGTAGAATGTATTGATGATGA
AATTACAGGTATTATAAAAGATTTGAAGGATACTTTATATGCAGGTACTGGTATTGGTCTTGCAGCACCTCAAATAGGTT
ATTTAAAAAGAATTTTCATTATAGATTTAAGAAATGGACAAGAACCTATAATTCTTATAAATCCTAAATTTTCAAAAAAA
ATAGGAAAAGAAGAAAGTGAAGAGGGGTGCTTAAGCTATCCAGGTTATGAAGGGATTGTTATTAGACCCAGAAGAGTTGC
TATAACGGGACTAAATGAGAAAGGTGAAGAAGTTACTTATGAAGCAACAGGCTTACTAAAGAATGCTTTTTGCCATGAGT
ATGATCATTTAGATGGCATAGTTTATATAGATAAGGCTAAGAAGGTATACAAAGTGGAACAAATAGAATAA

Upstream 100 bases:

>100_bases
TTCTTATAATAATCTCGCATTTTTCTAGAATATTATGTATAATTTAGTTATACATGATAAAAAGGAGGGAGTTTTAAAGC
TTTTAGCTTTAAGAATTATT

Downstream 100 bases:

>100_bases
AAAAGGGGAGAATGCTAATGCATAGGATTATTTATGACTGTGATAACACCATGGGGCTTAAAGATAAAGACGTGGATGAT
GGTCTAACTTTAATGTATCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MAIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDTLYAGTGIGLAAPQIGYLKRIFIIDLRNGQEPIILINPKFSKK
IGKEESEEGCLSYPGYEGIVIRPRRVAITGLNEKGEEVTYEATGLLKNAFCHEYDHLDGIVYIDKAKKVYKVEQIE

Sequences:

>Translated_156_residues
MAIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDTLYAGTGIGLAAPQIGYLKRIFIIDLRNGQEPIILINPKFSKK
IGKEESEEGCLSYPGYEGIVIRPRRVAITGLNEKGEEVTYEATGLLKNAFCHEYDHLDGIVYIDKAKKVYKVEQIE
>Mature_155_residues
AIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDTLYAGTGIGLAAPQIGYLKRIFIIDLRNGQEPIILINPKFSKKI
GKEESEEGCLSYPGYEGIVIRPRRVAITGLNEKGEEVTYEATGLLKNAFCHEYDHLDGIVYIDKAKKVYKVEQIE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=28.3950617283951, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1789682, Length=145, Percent_Identity=40.6896551724138, Blast_Score=111, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=31.5151515151515, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 17529; Mature: 17397

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDTLYAGTGIGLAAPQIGYLKRIFI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEE
IDLRNGQEPIILINPKFSKKIGKEESEEGCLSYPGYEGIVIRPRRVAITGLNEKGEEVTY
EEECCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCEEEH
EATGLLKNAFCHEYDHLDGIVYIDKAKKVYKVEQIE
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCC
>Mature Secondary Structure 
AIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDTLYAGTGIGLAAPQIGYLKRIFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEE
IDLRNGQEPIILINPKFSKKIGKEESEEGCLSYPGYEGIVIRPRRVAITGLNEKGEEVTY
EEECCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCEEEH
EATGLLKNAFCHEYDHLDGIVYIDKAKKVYKVEQIE
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]