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The map label for this gene is prdE [H]

Identifier: 148380431

GI number: 148380431

Start: 2611792

End: 2612262

Strand: Reverse

Name: prdE [H]

Synonym: CBO2475

Alternate gene names: 148380431

Gene position: 2612262-2611792 (Counterclockwise)

Preceding gene: 148380432

Following gene: 148380430

Centisome position: 67.21

GC content: 33.97

Gene sequence:

>471_bases
ATGGGTATAGGACCATCTACAAAAGAAACAACATTACATCATTTTAGAGACCCTTTAGTAGAAATAGTTTCTAATGACGG
AGATATAGATTTATTAGGAATTATAGTTGCAGGCACACCTCAAGCAAATGAAGAAAAGGTATTCACAGGCCAGAGGATAG
GAGCTTGGGCAGAGGCTATGAGAGCTGATGGCGCTATAGTATCAATAGATGGATGGGGCAATAGTAATATAGATTTTGCC
TCAGCTTTAGAAGCTATAGGTAAGAGAGATATACCTGTAGTTGGTATGAGTTTTGTAGGAACTCAAGCTCAATTTGTTGT
TACAAATGAATTTATGGATACAGTGGTTGATTTTAATAAATCAAAAGCAGGTATAGAAACTGAGGTTGTAGGAGAAAACA
ATGTTATGTCAATAGATGCTAAAAAAGCTTTAGCATTTTTAAAATTAAAAATGAAAAGAAAACAAAACTAA

Upstream 100 bases:

>100_bases
TGTAAATCTATAATAGATATGGGAAATATGCCAGTGGTTGTAAGTCCAAATGAATATAGAGATGGCATATTAAGAGCCAT
GAATTAAGGGGTGATATTTT

Downstream 100 bases:

>100_bases
GGAGGCTTTAATAATGAGAGCAATTAAAACAATTCAAACTATTGAATCTCACACAATGGGAGAACCAACAAGAATAGTAA
TAGGAGGACTTCCAAAGGTT

Product: PrdE protein

Products: NA

Alternate protein names: D-proline reductase 45 kDa subunit; D-proline reductase 23 kDa subunit [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MGIGPSTKETTLHHFRDPLVEIVSNDGDIDLLGIIVAGTPQANEEKVFTGQRIGAWAEAMRADGAIVSIDGWGNSNIDFA
SALEAIGKRDIPVVGMSFVGTQAQFVVTNEFMDTVVDFNKSKAGIETEVVGENNVMSIDAKKALAFLKLKMKRKQN

Sequences:

>Translated_156_residues
MGIGPSTKETTLHHFRDPLVEIVSNDGDIDLLGIIVAGTPQANEEKVFTGQRIGAWAEAMRADGAIVSIDGWGNSNIDFA
SALEAIGKRDIPVVGMSFVGTQAQFVVTNEFMDTVVDFNKSKAGIETEVVGENNVMSIDAKKALAFLKLKMKRKQN
>Mature_155_residues
GIGPSTKETTLHHFRDPLVEIVSNDGDIDLLGIIVAGTPQANEEKVFTGQRIGAWAEAMRADGAIVSIDGWGNSNIDFAS
ALEAIGKRDIPVVGMSFVGTQAQFVVTNEFMDTVVDFNKSKAGIETEVVGENNVMSIDAKKALAFLKLKMKRKQN

Specific function: D-proline reductase catalyzes the reductive cleavage of a C-N bond in D-proline resulting in the formation of 5- aminovalerate. The alpha subunit has been shown to bind D-proline, presumably via the pyruvoyl group [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.1 [H]

Molecular weight: Translated: 16825; Mature: 16694

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIGPSTKETTLHHFRDPLVEIVSNDGDIDLLGIIVAGTPQANEEKVFTGQRIGAWAEAM
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCEECCCHHHHHHHHH
RADGAIVSIDGWGNSNIDFASALEAIGKRDIPVVGMSFVGTQAQFVVTNEFMDTVVDFNK
HCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCEEEHEECCCCEEEEEEHHHHHHHHHCCC
SKAGIETEVVGENNVMSIDAKKALAFLKLKMKRKQN
CCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GIGPSTKETTLHHFRDPLVEIVSNDGDIDLLGIIVAGTPQANEEKVFTGQRIGAWAEAM
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCEECCCHHHHHHHHH
RADGAIVSIDGWGNSNIDFASALEAIGKRDIPVVGMSFVGTQAQFVVTNEFMDTVVDFNK
HCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCEEEHEECCCCEEEEEEHHHHHHHHHCCC
SKAGIETEVVGENNVMSIDAKKALAFLKLKMKRKQN
CCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10085076 [H]